Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23940 and BWI76_RS23945 are separated by 10 nucleotides BWI76_RS23945 and BWI76_RS23950 are separated by 24 nucleotides BWI76_RS23950 and BWI76_RS23955 overlap by 4 nucleotides
BWI76_RS23940: BWI76_RS23940 - ATPase/protein kinase, at 4,997,084 to 4,998,079
_RS23940
BWI76_RS23945: BWI76_RS23945 - methylmalonyl-CoA decarboxylase, at 4,998,090 to 4,998,875
_RS23945
BWI76_RS23950: BWI76_RS23950 - succinate CoA transferase, at 4,998,900 to 5,000,366
_RS23950
BWI76_RS23955: BWI76_RS23955 - LysR family transcriptional regulator, at 5,000,363 to 5,001,259
_RS23955
Position (kb) 4998
4999
5000
5001 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 4997.947 kb on + strand, within BWI76_RS23940 at 4998.334 kb on - strand, within BWI76_RS23945 at 4998.407 kb on + strand, within BWI76_RS23945 at 4998.806 kb on - strand at 4998.891 kb on - strand at 4998.891 kb on - strand at 4998.891 kb on - strand at 4999.206 kb on - strand, within BWI76_RS23950 at 4999.466 kb on + strand, within BWI76_RS23950 at 4999.603 kb on + strand, within BWI76_RS23950 at 4999.746 kb on - strand, within BWI76_RS23950 at 4999.746 kb on - strand, within BWI76_RS23950 at 4999.850 kb on - strand, within BWI76_RS23950 at 4999.873 kb on + strand, within BWI76_RS23950 at 4999.932 kb on - strand, within BWI76_RS23950 at 4999.980 kb on + strand, within BWI76_RS23950 at 4999.999 kb on + strand, within BWI76_RS23950 at 5000.007 kb on - strand, within BWI76_RS23950 at 5000.146 kb on + strand, within BWI76_RS23950 at 5000.199 kb on - strand, within BWI76_RS23950 at 5000.350 kb on + strand at 5000.350 kb on + strand at 5000.533 kb on - strand, within BWI76_RS23955 at 5000.579 kb on + strand, within BWI76_RS23955 at 5000.624 kb on + strand, within BWI76_RS23955 at 5000.851 kb on + strand, within BWI76_RS23955 at 5000.969 kb on + strand, within BWI76_RS23955 at 5001.080 kb on - strand, within BWI76_RS23955 at 5001.112 kb on + strand, within BWI76_RS23955
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 4,997,947 + BWI76_RS23940 0.87 -1.3 4,998,334 - BWI76_RS23945 0.31 +0.4 4,998,407 + BWI76_RS23945 0.40 +0.4 4,998,806 - +1.3 4,998,891 - -1.6 4,998,891 - -2.2 4,998,891 - -0.8 4,999,206 - BWI76_RS23950 0.21 -3.1 4,999,466 + BWI76_RS23950 0.39 +0.6 4,999,603 + BWI76_RS23950 0.48 +1.0 4,999,746 - BWI76_RS23950 0.58 +0.7 4,999,746 - BWI76_RS23950 0.58 +3.4 4,999,850 - BWI76_RS23950 0.65 -0.3 4,999,873 + BWI76_RS23950 0.66 +2.0 4,999,932 - BWI76_RS23950 0.70 -3.5 4,999,980 + BWI76_RS23950 0.74 +0.3 4,999,999 + BWI76_RS23950 0.75 -1.7 5,000,007 - BWI76_RS23950 0.75 +0.5 5,000,146 + BWI76_RS23950 0.85 +0.9 5,000,199 - BWI76_RS23950 0.89 +0.5 5,000,350 + -0.6 5,000,350 + -3.8 5,000,533 - BWI76_RS23955 0.19 +0.0 5,000,579 + BWI76_RS23955 0.24 -2.1 5,000,624 + BWI76_RS23955 0.29 -0.4 5,000,851 + BWI76_RS23955 0.54 +0.2 5,000,969 + BWI76_RS23955 0.68 +0.2 5,001,080 - BWI76_RS23955 0.80 +0.9 5,001,112 + BWI76_RS23955 0.84 -0.6
Or see this region's nucleotide sequence