Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21700 and BWI76_RS21705 are separated by 87 nucleotides BWI76_RS21705 and BWI76_RS21710 are separated by 171 nucleotides BWI76_RS21710 and BWI76_RS21715 overlap by 10 nucleotides BWI76_RS21715 and BWI76_RS21720 are separated by 1 nucleotides BWI76_RS21720 and BWI76_RS21725 overlap by 52 nucleotides
BWI76_RS21700: BWI76_RS21700 - AraC family transcriptional regulator, at 4,538,613 to 4,539,407
_RS21700
BWI76_RS21705: BWI76_RS21705 - MFS transporter, at 4,539,495 to 4,540,685
_RS21705
BWI76_RS21710: BWI76_RS21710 - nitroreductase, at 4,540,857 to 4,541,231
_RS21710
BWI76_RS21715: BWI76_RS21715 - transcriptional regulator, at 4,541,222 to 4,541,401
_RS21715
BWI76_RS21720: BWI76_RS21720 - hypothetical protein, at 4,541,403 to 4,541,645
_RS21720
BWI76_RS21725: BWI76_RS21725 - flavin reductase, at 4,541,594 to 4,542,166
_RS21725
Position (kb) 4539
4540
4541 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4538.611 kb on + strand at 4538.652 kb on - strand at 4538.662 kb on - strand at 4538.667 kb on - strand at 4538.703 kb on + strand, within BWI76_RS21700 at 4538.710 kb on + strand, within BWI76_RS21700 at 4538.715 kb on + strand, within BWI76_RS21700 at 4538.718 kb on + strand, within BWI76_RS21700 at 4538.723 kb on - strand, within BWI76_RS21700 at 4538.820 kb on + strand, within BWI76_RS21700 at 4538.849 kb on - strand, within BWI76_RS21700 at 4538.857 kb on - strand, within BWI76_RS21700 at 4538.895 kb on - strand, within BWI76_RS21700 at 4539.045 kb on + strand, within BWI76_RS21700 at 4539.168 kb on + strand, within BWI76_RS21700 at 4539.250 kb on - strand, within BWI76_RS21700 at 4539.369 kb on - strand at 4539.369 kb on - strand at 4539.374 kb on + strand at 4539.510 kb on + strand at 4539.626 kb on + strand, within BWI76_RS21705 at 4539.636 kb on - strand, within BWI76_RS21705 at 4539.682 kb on + strand, within BWI76_RS21705 at 4539.725 kb on - strand, within BWI76_RS21705 at 4539.725 kb on - strand, within BWI76_RS21705 at 4539.775 kb on + strand, within BWI76_RS21705 at 4539.788 kb on + strand, within BWI76_RS21705 at 4539.834 kb on + strand, within BWI76_RS21705 at 4539.842 kb on - strand, within BWI76_RS21705 at 4539.853 kb on + strand, within BWI76_RS21705 at 4539.943 kb on + strand, within BWI76_RS21705 at 4539.979 kb on - strand, within BWI76_RS21705 at 4540.116 kb on + strand, within BWI76_RS21705 at 4540.139 kb on + strand, within BWI76_RS21705 at 4540.212 kb on - strand, within BWI76_RS21705 at 4540.239 kb on - strand, within BWI76_RS21705 at 4540.404 kb on - strand, within BWI76_RS21705 at 4540.503 kb on + strand, within BWI76_RS21705 at 4540.689 kb on + strand at 4540.697 kb on - strand at 4540.697 kb on - strand at 4540.707 kb on - strand at 4540.724 kb on - strand at 4540.751 kb on - strand at 4540.772 kb on + strand at 4540.772 kb on + strand at 4540.772 kb on + strand at 4540.777 kb on + strand at 4540.782 kb on + strand at 4540.815 kb on + strand at 4540.855 kb on + strand at 4540.863 kb on - strand at 4540.863 kb on - strand at 4540.863 kb on - strand at 4540.868 kb on - strand at 4540.904 kb on - strand, within BWI76_RS21710 at 4540.925 kb on + strand, within BWI76_RS21710 at 4540.926 kb on - strand, within BWI76_RS21710 at 4541.051 kb on + strand, within BWI76_RS21710 at 4541.062 kb on + strand, within BWI76_RS21710 at 4541.083 kb on + strand, within BWI76_RS21710 at 4541.159 kb on + strand, within BWI76_RS21710 at 4541.249 kb on - strand, within BWI76_RS21715 at 4541.658 kb on + strand, within BWI76_RS21725 at 4541.673 kb on - strand, within BWI76_RS21725
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 4,538,611 + -0.4 4,538,652 - +1.2 4,538,662 - -1.2 4,538,667 - -1.2 4,538,703 + BWI76_RS21700 0.11 +0.0 4,538,710 + BWI76_RS21700 0.12 -1.5 4,538,715 + BWI76_RS21700 0.13 +0.9 4,538,718 + BWI76_RS21700 0.13 +1.4 4,538,723 - BWI76_RS21700 0.14 +0.9 4,538,820 + BWI76_RS21700 0.26 -0.1 4,538,849 - BWI76_RS21700 0.30 -0.7 4,538,857 - BWI76_RS21700 0.31 -1.7 4,538,895 - BWI76_RS21700 0.35 -0.1 4,539,045 + BWI76_RS21700 0.54 -0.4 4,539,168 + BWI76_RS21700 0.70 +0.9 4,539,250 - BWI76_RS21700 0.80 -0.1 4,539,369 - -0.3 4,539,369 - +0.7 4,539,374 + +1.1 4,539,510 + -0.5 4,539,626 + BWI76_RS21705 0.11 +0.1 4,539,636 - BWI76_RS21705 0.12 -1.9 4,539,682 + BWI76_RS21705 0.16 -2.2 4,539,725 - BWI76_RS21705 0.19 -1.7 4,539,725 - BWI76_RS21705 0.19 -0.1 4,539,775 + BWI76_RS21705 0.24 -1.5 4,539,788 + BWI76_RS21705 0.25 -1.5 4,539,834 + BWI76_RS21705 0.28 -3.1 4,539,842 - BWI76_RS21705 0.29 +0.5 4,539,853 + BWI76_RS21705 0.30 -0.6 4,539,943 + BWI76_RS21705 0.38 -0.4 4,539,979 - BWI76_RS21705 0.41 +0.9 4,540,116 + BWI76_RS21705 0.52 -1.0 4,540,139 + BWI76_RS21705 0.54 -0.9 4,540,212 - BWI76_RS21705 0.60 -0.6 4,540,239 - BWI76_RS21705 0.62 -1.0 4,540,404 - BWI76_RS21705 0.76 +0.8 4,540,503 + BWI76_RS21705 0.85 +0.2 4,540,689 + -1.8 4,540,697 - -2.9 4,540,697 - -2.8 4,540,707 - -2.0 4,540,724 - +0.3 4,540,751 - +0.6 4,540,772 + -3.4 4,540,772 + -1.6 4,540,772 + -2.9 4,540,777 + -2.1 4,540,782 + -1.9 4,540,815 + -4.9 4,540,855 + -1.4 4,540,863 - -0.2 4,540,863 - +0.8 4,540,863 - -1.5 4,540,868 - -3.1 4,540,904 - BWI76_RS21710 0.13 -0.1 4,540,925 + BWI76_RS21710 0.18 +0.0 4,540,926 - BWI76_RS21710 0.18 -1.4 4,541,051 + BWI76_RS21710 0.52 -1.4 4,541,062 + BWI76_RS21710 0.55 +0.9 4,541,083 + BWI76_RS21710 0.60 -0.8 4,541,159 + BWI76_RS21710 0.81 -3.1 4,541,249 - BWI76_RS21715 0.15 +0.1 4,541,658 + BWI76_RS21725 0.11 -0.4 4,541,673 - BWI76_RS21725 0.14 +0.3
Or see this region's nucleotide sequence