Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17410 and BWI76_RS17415 are separated by 89 nucleotides BWI76_RS17415 and BWI76_RS17420 are separated by 39 nucleotides BWI76_RS17420 and BWI76_RS17425 are separated by 3 nucleotides
BWI76_RS17410: BWI76_RS17410 - sodium-potassium/proton antiporter ChaA, at 3,611,392 to 3,612,492
_RS17410
BWI76_RS17415: BWI76_RS17415 - 3-deoxy-8-phosphooctulonate synthase, at 3,612,582 to 3,613,436
_RS17415
BWI76_RS17420: BWI76_RS17420 - hypothetical protein, at 3,613,476 to 3,614,288
_RS17420
BWI76_RS17425: BWI76_RS17425 - hypothetical protein, at 3,614,292 to 3,614,684
_RS17425
Position (kb)
3612
3613
3614 Strain fitness (log2 ratio)
-1
0
1 at 3611.807 kb on + strand, within BWI76_RS17410 at 3611.807 kb on + strand, within BWI76_RS17410 at 3611.807 kb on + strand, within BWI76_RS17410 at 3611.882 kb on - strand, within BWI76_RS17410 at 3613.611 kb on - strand, within BWI76_RS17420 at 3613.979 kb on - strand, within BWI76_RS17420 at 3614.032 kb on - strand, within BWI76_RS17420
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 3,611,807 + BWI76_RS17410 0.38 +0.5 3,611,807 + BWI76_RS17410 0.38 +0.7 3,611,807 + BWI76_RS17410 0.38 +1.0 3,611,882 - BWI76_RS17410 0.45 -1.7 3,613,611 - BWI76_RS17420 0.17 +1.2 3,613,979 - BWI76_RS17420 0.62 +1.3 3,614,032 - BWI76_RS17420 0.68 +1.7
Or see this region's nucleotide sequence