Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17260 and BWI76_RS17265 are separated by 93 nucleotides BWI76_RS17265 and BWI76_RS17270 are separated by 134 nucleotides BWI76_RS17270 and BWI76_RS17275 are separated by 197 nucleotides
BWI76_RS17260: BWI76_RS17260 - hypothetical protein, at 3,577,790 to 3,578,108
_RS17260
BWI76_RS17265: BWI76_RS17265 - DNA-binding protein, at 3,578,202 to 3,578,609
_RS17265
BWI76_RS17270: BWI76_RS17270 - UTP--glucose-1-phosphate uridylyltransferase, at 3,578,744 to 3,579,646
_RS17270
BWI76_RS17275: BWI76_RS17275 - two-component system response regulator RssB, at 3,579,844 to 3,580,857
_RS17275
Position (kb)
3578
3579
3580 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 3577.757 kb on - strand at 3577.759 kb on - strand at 3577.759 kb on - strand at 3577.773 kb on - strand at 3577.797 kb on + strand at 3577.802 kb on - strand at 3577.805 kb on - strand at 3577.816 kb on - strand at 3577.825 kb on + strand, within BWI76_RS17260 at 3577.840 kb on - strand, within BWI76_RS17260 at 3577.848 kb on - strand, within BWI76_RS17260 at 3577.848 kb on - strand, within BWI76_RS17260 at 3577.915 kb on - strand, within BWI76_RS17260 at 3577.945 kb on + strand, within BWI76_RS17260 at 3577.963 kb on - strand, within BWI76_RS17260 at 3577.963 kb on - strand, within BWI76_RS17260 at 3577.992 kb on - strand, within BWI76_RS17260 at 3578.014 kb on - strand, within BWI76_RS17260 at 3578.118 kb on + strand at 3578.119 kb on - strand at 3578.123 kb on + strand at 3578.123 kb on + strand at 3578.522 kb on + strand, within BWI76_RS17265 at 3578.673 kb on - strand at 3578.747 kb on - strand at 3579.564 kb on + strand at 3579.661 kb on - strand at 3579.661 kb on - strand at 3579.661 kb on - strand at 3579.704 kb on + strand at 3579.708 kb on - strand at 3579.712 kb on - strand at 3579.731 kb on - strand at 3579.795 kb on + strand at 3579.816 kb on - strand at 3579.816 kb on - strand at 3579.847 kb on - strand at 3579.859 kb on + strand at 3579.908 kb on - strand at 3579.931 kb on - strand at 3579.992 kb on + strand, within BWI76_RS17275 at 3579.992 kb on + strand, within BWI76_RS17275 at 3580.000 kb on - strand at 3580.000 kb on - strand, within BWI76_RS17275 at 3580.000 kb on - strand, within BWI76_RS17275 at 3580.022 kb on - strand, within BWI76_RS17275 at 3580.061 kb on - strand, within BWI76_RS17275 at 3580.088 kb on + strand, within BWI76_RS17275 at 3580.096 kb on - strand, within BWI76_RS17275 at 3580.117 kb on - strand, within BWI76_RS17275 at 3580.140 kb on - strand, within BWI76_RS17275 at 3580.142 kb on + strand, within BWI76_RS17275 at 3580.142 kb on + strand, within BWI76_RS17275 at 3580.150 kb on - strand, within BWI76_RS17275 at 3580.150 kb on - strand, within BWI76_RS17275 at 3580.150 kb on - strand, within BWI76_RS17275 at 3580.150 kb on - strand, within BWI76_RS17275 at 3580.184 kb on + strand, within BWI76_RS17275 at 3580.184 kb on + strand, within BWI76_RS17275 at 3580.184 kb on + strand, within BWI76_RS17275 at 3580.184 kb on + strand, within BWI76_RS17275 at 3580.192 kb on - strand, within BWI76_RS17275 at 3580.192 kb on - strand, within BWI76_RS17275 at 3580.192 kb on - strand, within BWI76_RS17275 at 3580.219 kb on - strand, within BWI76_RS17275 at 3580.299 kb on - strand, within BWI76_RS17275 at 3580.301 kb on + strand, within BWI76_RS17275 at 3580.373 kb on + strand, within BWI76_RS17275 at 3580.383 kb on + strand, within BWI76_RS17275 at 3580.400 kb on - strand, within BWI76_RS17275 at 3580.400 kb on - strand, within BWI76_RS17275 at 3580.569 kb on + strand, within BWI76_RS17275
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 3,577,757 - +1.8 3,577,759 - -0.2 3,577,759 - -2.5 3,577,773 - +2.2 3,577,797 + +2.1 3,577,802 - -0.7 3,577,805 - -1.7 3,577,816 - +0.9 3,577,825 + BWI76_RS17260 0.11 +2.1 3,577,840 - BWI76_RS17260 0.16 +0.6 3,577,848 - BWI76_RS17260 0.18 -2.1 3,577,848 - BWI76_RS17260 0.18 -1.1 3,577,915 - BWI76_RS17260 0.39 +0.8 3,577,945 + BWI76_RS17260 0.49 +0.7 3,577,963 - BWI76_RS17260 0.54 -1.0 3,577,963 - BWI76_RS17260 0.54 -0.9 3,577,992 - BWI76_RS17260 0.63 -3.1 3,578,014 - BWI76_RS17260 0.70 -0.2 3,578,118 + +0.1 3,578,119 - +1.1 3,578,123 + +1.4 3,578,123 + -0.1 3,578,522 + BWI76_RS17265 0.78 +2.1 3,578,673 - +1.1 3,578,747 - +0.6 3,579,564 + -1.2 3,579,661 - +1.2 3,579,661 - +1.0 3,579,661 - +5.2 3,579,704 + -0.2 3,579,708 - -3.0 3,579,712 - -0.1 3,579,731 - -0.0 3,579,795 + -1.4 3,579,816 - -0.7 3,579,816 - +0.1 3,579,847 - -0.1 3,579,859 + -0.7 3,579,908 - +1.6 3,579,931 - +2.5 3,579,992 + BWI76_RS17275 0.15 -0.6 3,579,992 + BWI76_RS17275 0.15 -1.8 3,580,000 - +1.1 3,580,000 - BWI76_RS17275 0.15 +0.9 3,580,000 - BWI76_RS17275 0.15 -1.1 3,580,022 - BWI76_RS17275 0.18 +0.2 3,580,061 - BWI76_RS17275 0.21 +0.2 3,580,088 + BWI76_RS17275 0.24 -2.5 3,580,096 - BWI76_RS17275 0.25 +1.3 3,580,117 - BWI76_RS17275 0.27 +1.1 3,580,140 - BWI76_RS17275 0.29 -1.6 3,580,142 + BWI76_RS17275 0.29 -0.1 3,580,142 + BWI76_RS17275 0.29 -2.4 3,580,150 - BWI76_RS17275 0.30 -2.7 3,580,150 - BWI76_RS17275 0.30 -2.8 3,580,150 - BWI76_RS17275 0.30 -1.9 3,580,150 - BWI76_RS17275 0.30 +0.0 3,580,184 + BWI76_RS17275 0.34 -3.6 3,580,184 + BWI76_RS17275 0.34 -1.1 3,580,184 + BWI76_RS17275 0.34 +0.6 3,580,184 + BWI76_RS17275 0.34 -1.6 3,580,192 - BWI76_RS17275 0.34 +0.5 3,580,192 - BWI76_RS17275 0.34 +2.1 3,580,192 - BWI76_RS17275 0.34 -0.9 3,580,219 - BWI76_RS17275 0.37 -1.6 3,580,299 - BWI76_RS17275 0.45 +1.1 3,580,301 + BWI76_RS17275 0.45 -1.2 3,580,373 + BWI76_RS17275 0.52 +0.4 3,580,383 + BWI76_RS17275 0.53 -0.5 3,580,400 - BWI76_RS17275 0.55 -0.2 3,580,400 - BWI76_RS17275 0.55 -0.1 3,580,569 + BWI76_RS17275 0.71 -0.0
Or see this region's nucleotide sequence