Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17150 and BWI76_RS17155 overlap by 39 nucleotides BWI76_RS17155 and BWI76_RS17160 are separated by 42 nucleotides BWI76_RS17160 and BWI76_RS17165 are separated by 14 nucleotides BWI76_RS17165 and BWI76_RS17170 are separated by 270 nucleotides BWI76_RS17170 and BWI76_RS17175 are separated by 289 nucleotides
BWI76_RS17150: BWI76_RS17150 - threonine--tRNA ligase, at 3,556,079 to 3,558,007
_RS17150
BWI76_RS17155: BWI76_RS17155 - hypothetical protein, at 3,557,969 to 3,558,181
_RS17155
BWI76_RS17160: BWI76_RS17160 - hypothetical protein, at 3,558,224 to 3,558,442
_RS17160
BWI76_RS17165: BWI76_RS17165 - hypothetical protein, at 3,558,457 to 3,558,690
_RS17165
BWI76_RS17170: BWI76_RS17170 - membrane protein, at 3,558,961 to 3,559,719
_RS17170
BWI76_RS17175: BWI76_RS17175 - 6-phosphofructokinase II, at 3,560,009 to 3,560,941
_RS17175
Position (kb)
3558
3559
3560 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3558.090 kb on - strand, within BWI76_RS17155 at 3558.122 kb on - strand, within BWI76_RS17155 at 3558.183 kb on - strand at 3558.188 kb on + strand at 3558.188 kb on + strand at 3558.188 kb on + strand at 3558.188 kb on + strand at 3558.188 kb on + strand at 3558.196 kb on - strand at 3558.196 kb on - strand at 3558.227 kb on - strand at 3558.308 kb on - strand, within BWI76_RS17160 at 3558.310 kb on + strand, within BWI76_RS17160 at 3558.333 kb on + strand, within BWI76_RS17160 at 3558.395 kb on + strand, within BWI76_RS17160 at 3558.395 kb on + strand, within BWI76_RS17160 at 3558.395 kb on + strand, within BWI76_RS17160 at 3558.395 kb on + strand, within BWI76_RS17160 at 3558.398 kb on - strand, within BWI76_RS17160 at 3558.402 kb on - strand, within BWI76_RS17160 at 3558.403 kb on - strand, within BWI76_RS17160 at 3558.403 kb on - strand, within BWI76_RS17160 at 3558.403 kb on - strand, within BWI76_RS17160 at 3558.403 kb on - strand, within BWI76_RS17160 at 3558.405 kb on + strand, within BWI76_RS17160 at 3558.405 kb on + strand, within BWI76_RS17160 at 3558.413 kb on - strand, within BWI76_RS17160 at 3558.443 kb on - strand at 3558.454 kb on - strand at 3558.455 kb on + strand at 3558.463 kb on + strand at 3558.472 kb on + strand at 3558.480 kb on + strand at 3558.502 kb on + strand, within BWI76_RS17165 at 3558.503 kb on + strand, within BWI76_RS17165 at 3558.511 kb on - strand, within BWI76_RS17165 at 3558.521 kb on - strand, within BWI76_RS17165 at 3558.534 kb on + strand, within BWI76_RS17165 at 3558.541 kb on + strand at 3558.547 kb on - strand, within BWI76_RS17165 at 3558.549 kb on - strand, within BWI76_RS17165 at 3558.577 kb on + strand, within BWI76_RS17165 at 3558.577 kb on + strand, within BWI76_RS17165 at 3558.592 kb on + strand, within BWI76_RS17165 at 3558.643 kb on - strand, within BWI76_RS17165 at 3558.643 kb on - strand, within BWI76_RS17165 at 3558.647 kb on + strand, within BWI76_RS17165 at 3558.652 kb on - strand, within BWI76_RS17165 at 3558.655 kb on - strand, within BWI76_RS17165 at 3558.736 kb on + strand at 3558.736 kb on + strand at 3558.744 kb on - strand at 3558.744 kb on - strand at 3558.744 kb on - strand at 3558.746 kb on + strand at 3558.746 kb on + strand at 3558.746 kb on + strand at 3558.746 kb on + strand at 3558.746 kb on + strand at 3558.746 kb on + strand at 3558.746 kb on + strand at 3558.746 kb on + strand at 3558.748 kb on + strand at 3558.754 kb on + strand at 3558.754 kb on - strand at 3558.754 kb on - strand at 3558.754 kb on - strand at 3558.756 kb on + strand at 3558.756 kb on + strand at 3558.756 kb on + strand at 3558.756 kb on + strand at 3558.756 kb on + strand at 3558.756 kb on + strand at 3558.756 kb on + strand at 3558.756 kb on - strand at 3558.759 kb on - strand at 3558.764 kb on - strand at 3558.764 kb on - strand at 3558.764 kb on - strand at 3558.764 kb on - strand at 3558.764 kb on - strand at 3558.764 kb on - strand at 3558.764 kb on - strand at 3558.764 kb on - strand at 3558.940 kb on + strand at 3558.989 kb on - strand at 3558.989 kb on - strand at 3559.005 kb on - strand at 3559.013 kb on - strand at 3559.016 kb on + strand at 3559.031 kb on + strand at 3559.034 kb on - strand at 3559.044 kb on + strand, within BWI76_RS17170 at 3559.053 kb on - strand, within BWI76_RS17170 at 3559.055 kb on + strand, within BWI76_RS17170 at 3559.087 kb on + strand, within BWI76_RS17170 at 3559.087 kb on + strand, within BWI76_RS17170 at 3559.087 kb on + strand, within BWI76_RS17170 at 3559.105 kb on - strand, within BWI76_RS17170 at 3559.105 kb on - strand, within BWI76_RS17170 at 3559.146 kb on + strand, within BWI76_RS17170 at 3559.248 kb on + strand, within BWI76_RS17170 at 3559.278 kb on + strand, within BWI76_RS17170 at 3559.311 kb on - strand, within BWI76_RS17170 at 3559.348 kb on - strand, within BWI76_RS17170 at 3559.398 kb on + strand, within BWI76_RS17170 at 3559.514 kb on + strand, within BWI76_RS17170 at 3559.615 kb on - strand, within BWI76_RS17170 at 3559.626 kb on - strand, within BWI76_RS17170 at 3559.750 kb on + strand at 3559.753 kb on - strand at 3559.770 kb on - strand at 3559.852 kb on - strand at 3559.874 kb on + strand at 3560.031 kb on + strand at 3560.039 kb on - strand at 3560.041 kb on + strand at 3560.140 kb on + strand, within BWI76_RS17175
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 3,558,090 - BWI76_RS17155 0.57 -0.4 3,558,122 - BWI76_RS17155 0.72 -3.1 3,558,183 - -2.7 3,558,188 + +1.2 3,558,188 + +0.3 3,558,188 + -1.3 3,558,188 + -0.4 3,558,188 + +1.2 3,558,196 - -0.4 3,558,196 - +0.0 3,558,227 - +0.6 3,558,308 - BWI76_RS17160 0.38 -1.6 3,558,310 + BWI76_RS17160 0.39 +1.1 3,558,333 + BWI76_RS17160 0.50 -2.9 3,558,395 + BWI76_RS17160 0.78 -0.3 3,558,395 + BWI76_RS17160 0.78 +0.6 3,558,395 + BWI76_RS17160 0.78 +1.7 3,558,395 + BWI76_RS17160 0.78 +1.3 3,558,398 - BWI76_RS17160 0.79 -0.6 3,558,402 - BWI76_RS17160 0.81 -1.9 3,558,403 - BWI76_RS17160 0.82 +0.6 3,558,403 - BWI76_RS17160 0.82 -1.0 3,558,403 - BWI76_RS17160 0.82 -2.3 3,558,403 - BWI76_RS17160 0.82 -0.8 3,558,405 + BWI76_RS17160 0.83 +1.1 3,558,405 + BWI76_RS17160 0.83 -0.2 3,558,413 - BWI76_RS17160 0.86 -1.0 3,558,443 - +0.5 3,558,454 - +0.4 3,558,455 + +2.4 3,558,463 + +0.7 3,558,472 + -0.4 3,558,480 + +0.6 3,558,502 + BWI76_RS17165 0.19 +0.8 3,558,503 + BWI76_RS17165 0.20 -1.7 3,558,511 - BWI76_RS17165 0.23 +0.3 3,558,521 - BWI76_RS17165 0.27 -1.0 3,558,534 + BWI76_RS17165 0.33 -0.4 3,558,541 + +2.1 3,558,547 - BWI76_RS17165 0.38 -1.6 3,558,549 - BWI76_RS17165 0.39 -0.3 3,558,577 + BWI76_RS17165 0.51 -2.1 3,558,577 + BWI76_RS17165 0.51 +0.3 3,558,592 + BWI76_RS17165 0.58 -2.6 3,558,643 - BWI76_RS17165 0.79 +2.1 3,558,643 - BWI76_RS17165 0.79 +0.1 3,558,647 + BWI76_RS17165 0.81 +0.6 3,558,652 - BWI76_RS17165 0.83 +0.6 3,558,655 - BWI76_RS17165 0.85 +1.1 3,558,736 + -0.0 3,558,736 + +0.4 3,558,744 - +1.8 3,558,744 - +2.1 3,558,744 - +0.1 3,558,746 + +3.0 3,558,746 + -1.2 3,558,746 + +2.7 3,558,746 + +0.3 3,558,746 + +1.6 3,558,746 + -0.8 3,558,746 + +0.7 3,558,746 + +1.6 3,558,748 + +0.2 3,558,754 + -3.4 3,558,754 - +1.3 3,558,754 - +1.1 3,558,754 - +0.6 3,558,756 + -2.4 3,558,756 + -0.1 3,558,756 + +1.7 3,558,756 + +0.0 3,558,756 + -2.1 3,558,756 + +0.4 3,558,756 + +2.1 3,558,756 - +0.5 3,558,759 - +0.3 3,558,764 - -0.4 3,558,764 - +0.6 3,558,764 - +0.2 3,558,764 - -1.6 3,558,764 - -2.2 3,558,764 - -0.6 3,558,764 - -2.4 3,558,764 - -1.2 3,558,940 + -1.7 3,558,989 - -0.3 3,558,989 - -0.9 3,559,005 - -2.5 3,559,013 - -5.2 3,559,016 + +0.9 3,559,031 + +2.4 3,559,034 - -0.4 3,559,044 + BWI76_RS17170 0.11 -0.5 3,559,053 - BWI76_RS17170 0.12 +0.7 3,559,055 + BWI76_RS17170 0.12 +0.1 3,559,087 + BWI76_RS17170 0.17 -0.9 3,559,087 + BWI76_RS17170 0.17 -1.4 3,559,087 + BWI76_RS17170 0.17 -0.2 3,559,105 - BWI76_RS17170 0.19 -2.6 3,559,105 - BWI76_RS17170 0.19 +1.5 3,559,146 + BWI76_RS17170 0.24 -0.3 3,559,248 + BWI76_RS17170 0.38 +0.7 3,559,278 + BWI76_RS17170 0.42 +0.3 3,559,311 - BWI76_RS17170 0.46 -0.1 3,559,348 - BWI76_RS17170 0.51 -2.6 3,559,398 + BWI76_RS17170 0.58 +0.1 3,559,514 + BWI76_RS17170 0.73 -1.2 3,559,615 - BWI76_RS17170 0.86 -0.5 3,559,626 - BWI76_RS17170 0.88 +1.5 3,559,750 + -3.1 3,559,753 - +0.3 3,559,770 - +2.7 3,559,852 - +1.3 3,559,874 + -1.9 3,560,031 + -1.6 3,560,039 - +0.4 3,560,041 + +0.8 3,560,140 + BWI76_RS17175 0.14 +1.9
Or see this region's nucleotide sequence