Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16735

Experiment: KLEB17 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16730 and BWI76_RS16735 are separated by 809 nucleotidesBWI76_RS16735 and BWI76_RS16740 are separated by 48 nucleotides BWI76_RS16730: BWI76_RS16730 - cystathionine gamma-lyase, at 3,465,235 to 3,466,422 _RS16730 BWI76_RS16735: BWI76_RS16735 - cold-shock protein, at 3,467,232 to 3,467,444 _RS16735 BWI76_RS16740: BWI76_RS16740 - transcriptional regulator, at 3,467,493 to 3,468,365 _RS16740 Position (kb) 3467 3468Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3466.233 kb on + strand, within BWI76_RS16730at 3466.240 kb on + strand, within BWI76_RS16730at 3466.247 kb on + strand, within BWI76_RS16730at 3466.247 kb on + strand, within BWI76_RS16730at 3466.255 kb on + strand, within BWI76_RS16730at 3466.255 kb on - strand, within BWI76_RS16730at 3466.255 kb on - strand, within BWI76_RS16730at 3466.255 kb on - strand, within BWI76_RS16730at 3466.259 kb on + strand, within BWI76_RS16730at 3466.267 kb on - strand, within BWI76_RS16730at 3466.269 kb on + strand, within BWI76_RS16730at 3466.292 kb on + strand, within BWI76_RS16730at 3466.292 kb on + strand, within BWI76_RS16730at 3466.292 kb on + strand, within BWI76_RS16730at 3466.485 kb on - strandat 3466.498 kb on - strandat 3466.569 kb on - strandat 3466.757 kb on + strandat 3466.763 kb on + strandat 3466.776 kb on - strandat 3466.791 kb on + strandat 3466.799 kb on - strandat 3466.819 kb on + strandat 3466.837 kb on - strandat 3466.853 kb on + strandat 3466.861 kb on + strandat 3466.861 kb on + strandat 3466.869 kb on + strandat 3466.945 kb on + strandat 3466.947 kb on + strandat 3466.963 kb on - strandat 3466.963 kb on - strandat 3466.963 kb on - strandat 3466.964 kb on + strandat 3466.964 kb on + strandat 3466.965 kb on - strandat 3466.969 kb on + strandat 3466.969 kb on + strandat 3466.969 kb on + strandat 3466.972 kb on - strandat 3466.977 kb on - strandat 3467.021 kb on + strandat 3467.029 kb on - strandat 3467.031 kb on - strandat 3467.073 kb on + strandat 3467.080 kb on - strandat 3467.184 kb on - strandat 3467.191 kb on + strandat 3467.199 kb on - strandat 3467.199 kb on - strandat 3467.199 kb on - strandat 3467.201 kb on - strandat 3467.201 kb on - strandat 3467.286 kb on + strand, within BWI76_RS16735at 3467.366 kb on - strand, within BWI76_RS16735at 3467.368 kb on + strand, within BWI76_RS16735at 3467.404 kb on - strand, within BWI76_RS16735at 3467.406 kb on + strand, within BWI76_RS16735at 3467.542 kb on - strandat 3467.583 kb on - strand, within BWI76_RS16740at 3467.764 kb on - strand, within BWI76_RS16740at 3468.038 kb on + strand, within BWI76_RS16740at 3468.038 kb on + strand, within BWI76_RS16740at 3468.205 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB17 MOI 1
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3,466,233 + BWI76_RS16730 0.84 -0.6
3,466,240 + BWI76_RS16730 0.85 -0.4
3,466,247 + BWI76_RS16730 0.85 -0.6
3,466,247 + BWI76_RS16730 0.85 +1.1
3,466,255 + BWI76_RS16730 0.86 -0.8
3,466,255 - BWI76_RS16730 0.86 -3.0
3,466,255 - BWI76_RS16730 0.86 +1.2
3,466,255 - BWI76_RS16730 0.86 -0.4
3,466,259 + BWI76_RS16730 0.86 -0.8
3,466,267 - BWI76_RS16730 0.87 -2.0
3,466,269 + BWI76_RS16730 0.87 +1.2
3,466,292 + BWI76_RS16730 0.89 -3.9
3,466,292 + BWI76_RS16730 0.89 -0.4
3,466,292 + BWI76_RS16730 0.89 +2.7
3,466,485 - -0.9
3,466,498 - -0.3
3,466,569 - -1.8
3,466,757 + -2.5
3,466,763 + -0.1
3,466,776 - -2.1
3,466,791 + +0.6
3,466,799 - +1.2
3,466,819 + -0.8
3,466,837 - +0.0
3,466,853 + +0.2
3,466,861 + +0.3
3,466,861 + +0.9
3,466,869 + -0.4
3,466,945 + -3.1
3,466,947 + -1.1
3,466,963 - +0.4
3,466,963 - +1.2
3,466,963 - +0.2
3,466,964 + -2.4
3,466,964 + -0.6
3,466,965 - -0.8
3,466,969 + -0.6
3,466,969 + -1.0
3,466,969 + -1.0
3,466,972 - +1.2
3,466,977 - -1.0
3,467,021 + +0.5
3,467,029 - -0.2
3,467,031 - +0.6
3,467,073 + +0.6
3,467,080 - -0.6
3,467,184 - +1.6
3,467,191 + -1.4
3,467,199 - +0.4
3,467,199 - -0.0
3,467,199 - -1.2
3,467,201 - -2.1
3,467,201 - -1.4
3,467,286 + BWI76_RS16735 0.25 +0.2
3,467,366 - BWI76_RS16735 0.63 -1.4
3,467,368 + BWI76_RS16735 0.64 -2.7
3,467,404 - BWI76_RS16735 0.81 -0.1
3,467,406 + BWI76_RS16735 0.82 -2.4
3,467,542 - -2.3
3,467,583 - BWI76_RS16740 0.10 +1.2
3,467,764 - BWI76_RS16740 0.31 -0.3
3,468,038 + BWI76_RS16740 0.62 -2.6
3,468,038 + BWI76_RS16740 0.62 -0.2
3,468,205 - +0.2

Or see this region's nucleotide sequence