Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16030 and BWI76_RS16035 overlap by 8 nucleotides BWI76_RS16035 and BWI76_RS16040 overlap by 1 nucleotides BWI76_RS16040 and BWI76_RS16045 are separated by 129 nucleotides
BWI76_RS16030: BWI76_RS16030 - malonate decarboxylase acyl carrier protein, at 3,324,632 to 3,324,931
_RS16030
BWI76_RS16035: BWI76_RS16035 - biotin-independent malonate decarboxylase subunit beta, at 3,324,924 to 3,325,757
_RS16035
BWI76_RS16040: BWI76_RS16040 - biotin-independent malonate decarboxylase subunit gamma, at 3,325,757 to 3,326,557
_RS16040
BWI76_RS16045: BWI76_RS16045 - transporter, at 3,326,687 to 3,327,646
_RS16045
Position (kb)
3325
3326
3327 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3324.776 kb on - strand, within BWI76_RS16030 at 3324.906 kb on + strand at 3324.991 kb on - strand at 3325.238 kb on + strand, within BWI76_RS16035 at 3325.287 kb on - strand, within BWI76_RS16035 at 3325.339 kb on + strand, within BWI76_RS16035 at 3326.371 kb on + strand, within BWI76_RS16040 at 3326.379 kb on - strand, within BWI76_RS16040 at 3326.517 kb on + strand at 3326.525 kb on - strand at 3326.580 kb on - strand at 3326.582 kb on + strand at 3326.841 kb on - strand, within BWI76_RS16045 at 3327.330 kb on + strand, within BWI76_RS16045 at 3327.471 kb on + strand, within BWI76_RS16045
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 3,324,776 - BWI76_RS16030 0.48 +0.6 3,324,906 + -0.6 3,324,991 - -2.1 3,325,238 + BWI76_RS16035 0.38 -0.3 3,325,287 - BWI76_RS16035 0.44 +0.8 3,325,339 + BWI76_RS16035 0.50 +2.4 3,326,371 + BWI76_RS16040 0.77 +2.3 3,326,379 - BWI76_RS16040 0.78 +1.5 3,326,517 + -0.3 3,326,525 - -1.2 3,326,580 - +0.8 3,326,582 + -0.5 3,326,841 - BWI76_RS16045 0.16 +0.1 3,327,330 + BWI76_RS16045 0.67 -1.0 3,327,471 + BWI76_RS16045 0.82 -0.2
Or see this region's nucleotide sequence