Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12970 and BWI76_RS12975 are separated by 54 nucleotides BWI76_RS12975 and BWI76_RS12980 are separated by 78 nucleotides
BWI76_RS12970: BWI76_RS12970 - MFS transporter, at 2,703,762 to 2,705,198
_RS12970
BWI76_RS12975: BWI76_RS12975 - type I glyceraldehyde-3-phosphate dehydrogenase, at 2,705,253 to 2,706,263
_RS12975
BWI76_RS12980: BWI76_RS12980 - AraC family transcriptional regulator, at 2,706,342 to 2,707,322
_RS12980
Position (kb)
2705
2706
2707 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2704.340 kb on + strand, within BWI76_RS12970 at 2704.441 kb on + strand, within BWI76_RS12970 at 2704.556 kb on + strand, within BWI76_RS12970 at 2704.587 kb on - strand, within BWI76_RS12970 at 2704.587 kb on - strand, within BWI76_RS12970 at 2704.593 kb on - strand, within BWI76_RS12970 at 2704.597 kb on - strand, within BWI76_RS12970 at 2704.681 kb on - strand, within BWI76_RS12970 at 2704.742 kb on + strand, within BWI76_RS12970 at 2704.757 kb on - strand at 2704.779 kb on - strand, within BWI76_RS12970 at 2704.815 kb on - strand, within BWI76_RS12970 at 2705.019 kb on + strand, within BWI76_RS12970 at 2705.019 kb on + strand, within BWI76_RS12970 at 2705.019 kb on + strand, within BWI76_RS12970 at 2705.506 kb on - strand, within BWI76_RS12975 at 2705.506 kb on - strand, within BWI76_RS12975 at 2705.747 kb on - strand, within BWI76_RS12975 at 2705.932 kb on + strand, within BWI76_RS12975 at 2706.272 kb on + strand at 2706.482 kb on + strand, within BWI76_RS12980 at 2706.686 kb on + strand, within BWI76_RS12980 at 2706.747 kb on + strand, within BWI76_RS12980 at 2706.893 kb on - strand, within BWI76_RS12980 at 2706.929 kb on + strand, within BWI76_RS12980 at 2707.157 kb on + strand, within BWI76_RS12980 at 2707.214 kb on + strand, within BWI76_RS12980 at 2707.215 kb on - strand, within BWI76_RS12980 at 2707.237 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 2,704,340 + BWI76_RS12970 0.40 +0.0 2,704,441 + BWI76_RS12970 0.47 -0.2 2,704,556 + BWI76_RS12970 0.55 -2.1 2,704,587 - BWI76_RS12970 0.57 -0.1 2,704,587 - BWI76_RS12970 0.57 -2.5 2,704,593 - BWI76_RS12970 0.58 -1.9 2,704,597 - BWI76_RS12970 0.58 +1.5 2,704,681 - BWI76_RS12970 0.64 -1.4 2,704,742 + BWI76_RS12970 0.68 -0.9 2,704,757 - -1.2 2,704,779 - BWI76_RS12970 0.71 -2.3 2,704,815 - BWI76_RS12970 0.73 -1.1 2,705,019 + BWI76_RS12970 0.87 -0.1 2,705,019 + BWI76_RS12970 0.87 -2.5 2,705,019 + BWI76_RS12970 0.87 -0.5 2,705,506 - BWI76_RS12975 0.25 -3.0 2,705,506 - BWI76_RS12975 0.25 -2.7 2,705,747 - BWI76_RS12975 0.49 +1.4 2,705,932 + BWI76_RS12975 0.67 -1.9 2,706,272 + -1.3 2,706,482 + BWI76_RS12980 0.14 -2.2 2,706,686 + BWI76_RS12980 0.35 +0.6 2,706,747 + BWI76_RS12980 0.41 -1.2 2,706,893 - BWI76_RS12980 0.56 +1.9 2,706,929 + BWI76_RS12980 0.60 -2.4 2,707,157 + BWI76_RS12980 0.83 -1.8 2,707,214 + BWI76_RS12980 0.89 -0.7 2,707,215 - BWI76_RS12980 0.89 -1.2 2,707,237 - +0.1
Or see this region's nucleotide sequence