Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12620 and BWI76_RS12625 are separated by 102 nucleotides BWI76_RS12625 and BWI76_RS12630 are separated by 140 nucleotides
BWI76_RS12620: BWI76_RS12620 - 2-nitropropane dioxygenase, at 2,634,019 to 2,634,999
_RS12620
BWI76_RS12625: BWI76_RS12625 - isoflavone reductase, at 2,635,102 to 2,636,031
_RS12625
BWI76_RS12630: BWI76_RS12630 - regulatory protein, LysR:LysR, substrate-binding protein, at 2,636,172 to 2,637,077
_RS12630
Position (kb)
2635
2636
2637 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2634.265 kb on - strand, within BWI76_RS12620 at 2634.265 kb on - strand, within BWI76_RS12620 at 2634.347 kb on + strand, within BWI76_RS12620 at 2634.382 kb on - strand, within BWI76_RS12620 at 2634.382 kb on - strand, within BWI76_RS12620 at 2634.382 kb on - strand, within BWI76_RS12620 at 2634.392 kb on - strand, within BWI76_RS12620 at 2634.621 kb on + strand, within BWI76_RS12620 at 2634.907 kb on + strand at 2635.013 kb on - strand at 2635.044 kb on + strand at 2635.131 kb on - strand at 2635.194 kb on - strand at 2635.196 kb on - strand, within BWI76_RS12625 at 2635.270 kb on - strand, within BWI76_RS12625 at 2635.287 kb on + strand, within BWI76_RS12625 at 2635.319 kb on - strand, within BWI76_RS12625 at 2635.365 kb on + strand, within BWI76_RS12625 at 2635.425 kb on - strand, within BWI76_RS12625 at 2635.438 kb on - strand, within BWI76_RS12625 at 2635.558 kb on + strand, within BWI76_RS12625 at 2635.698 kb on - strand, within BWI76_RS12625 at 2635.709 kb on + strand, within BWI76_RS12625 at 2636.086 kb on - strand at 2636.165 kb on - strand at 2636.446 kb on - strand, within BWI76_RS12630 at 2636.560 kb on + strand, within BWI76_RS12630 at 2636.583 kb on + strand, within BWI76_RS12630 at 2636.583 kb on + strand, within BWI76_RS12630 at 2636.583 kb on + strand, within BWI76_RS12630 at 2636.854 kb on + strand, within BWI76_RS12630 at 2636.862 kb on - strand, within BWI76_RS12630 at 2636.975 kb on - strand, within BWI76_RS12630
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 2,634,265 - BWI76_RS12620 0.25 +0.7 2,634,265 - BWI76_RS12620 0.25 -2.0 2,634,347 + BWI76_RS12620 0.33 -0.5 2,634,382 - BWI76_RS12620 0.37 +0.0 2,634,382 - BWI76_RS12620 0.37 +1.8 2,634,382 - BWI76_RS12620 0.37 +0.7 2,634,392 - BWI76_RS12620 0.38 -0.1 2,634,621 + BWI76_RS12620 0.61 -0.4 2,634,907 + +0.8 2,635,013 - -1.2 2,635,044 + -0.3 2,635,131 - -1.0 2,635,194 - +0.3 2,635,196 - BWI76_RS12625 0.10 +1.1 2,635,270 - BWI76_RS12625 0.18 +0.7 2,635,287 + BWI76_RS12625 0.20 +1.8 2,635,319 - BWI76_RS12625 0.23 +1.3 2,635,365 + BWI76_RS12625 0.28 -0.3 2,635,425 - BWI76_RS12625 0.35 +1.3 2,635,438 - BWI76_RS12625 0.36 +0.9 2,635,558 + BWI76_RS12625 0.49 +0.5 2,635,698 - BWI76_RS12625 0.64 +0.5 2,635,709 + BWI76_RS12625 0.65 -1.7 2,636,086 - -0.6 2,636,165 - -0.3 2,636,446 - BWI76_RS12630 0.30 -0.3 2,636,560 + BWI76_RS12630 0.43 -0.3 2,636,583 + BWI76_RS12630 0.45 +0.2 2,636,583 + BWI76_RS12630 0.45 -0.4 2,636,583 + BWI76_RS12630 0.45 +0.1 2,636,854 + BWI76_RS12630 0.75 +1.6 2,636,862 - BWI76_RS12630 0.76 -2.1 2,636,975 - BWI76_RS12630 0.89 -1.5
Or see this region's nucleotide sequence