Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12385 and BWI76_RS12390 are separated by 135 nucleotides BWI76_RS12390 and BWI76_RS12395 are separated by 349 nucleotides
BWI76_RS12385: BWI76_RS12385 - DNA polymerase V subunit UmuC, at 2,586,868 to 2,588,133
_RS12385
BWI76_RS12390: BWI76_RS12390 - antibiotic biosynthesis monooxygenase, at 2,588,269 to 2,588,568
_RS12390
BWI76_RS12395: BWI76_RS12395 - zinc-binding alcohol dehydrogenase, at 2,588,918 to 2,589,901
_RS12395
Position (kb)
2588
2589 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2587.287 kb on + strand, within BWI76_RS12385 at 2587.321 kb on + strand, within BWI76_RS12385 at 2587.367 kb on + strand, within BWI76_RS12385 at 2587.471 kb on + strand, within BWI76_RS12385 at 2587.548 kb on - strand, within BWI76_RS12385 at 2587.560 kb on + strand, within BWI76_RS12385 at 2587.568 kb on - strand at 2587.810 kb on + strand, within BWI76_RS12385 at 2587.963 kb on + strand, within BWI76_RS12385 at 2588.096 kb on + strand at 2588.104 kb on - strand at 2588.116 kb on - strand at 2588.230 kb on - strand at 2588.365 kb on + strand at 2588.435 kb on + strand, within BWI76_RS12390 at 2588.494 kb on + strand, within BWI76_RS12390 at 2588.624 kb on - strand at 2588.624 kb on - strand at 2588.649 kb on - strand at 2588.675 kb on - strand at 2588.710 kb on + strand at 2588.951 kb on + strand at 2588.994 kb on - strand at 2589.188 kb on - strand, within BWI76_RS12395 at 2589.353 kb on + strand, within BWI76_RS12395 at 2589.381 kb on - strand, within BWI76_RS12395 at 2589.495 kb on + strand, within BWI76_RS12395
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 2,587,287 + BWI76_RS12385 0.33 +1.0 2,587,321 + BWI76_RS12385 0.36 -0.1 2,587,367 + BWI76_RS12385 0.39 +2.3 2,587,471 + BWI76_RS12385 0.48 -2.3 2,587,548 - BWI76_RS12385 0.54 +0.7 2,587,560 + BWI76_RS12385 0.55 +2.3 2,587,568 - +1.3 2,587,810 + BWI76_RS12385 0.74 -2.1 2,587,963 + BWI76_RS12385 0.86 -0.7 2,588,096 + -1.6 2,588,104 - -0.1 2,588,116 - +1.4 2,588,230 - -1.7 2,588,365 + -0.6 2,588,435 + BWI76_RS12390 0.55 -0.3 2,588,494 + BWI76_RS12390 0.75 -3.7 2,588,624 - -2.1 2,588,624 - -2.1 2,588,649 - +0.2 2,588,675 - +1.7 2,588,710 + -1.6 2,588,951 + +0.6 2,588,994 - -3.0 2,589,188 - BWI76_RS12395 0.27 -1.1 2,589,353 + BWI76_RS12395 0.44 +0.7 2,589,381 - BWI76_RS12395 0.47 +2.4 2,589,495 + BWI76_RS12395 0.59 +1.1
Or see this region's nucleotide sequence