Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10445 and BWI76_RS10450 are separated by 239 nucleotides BWI76_RS10450 and BWI76_RS10455 are separated by 346 nucleotides BWI76_RS10455 and BWI76_RS10460 are separated by 21 nucleotides
BWI76_RS10445: BWI76_RS10445 - hypothetical protein, at 2,208,481 to 2,208,861
_RS10445
BWI76_RS10450: BWI76_RS10450 - tRNA-Tyr, at 2,209,101 to 2,209,175
_RS10450
BWI76_RS10455: BWI76_RS10455 - hypothetical protein, at 2,209,522 to 2,209,728
_RS10455
BWI76_RS10460: BWI76_RS10460 - transcriptional regulator LeuO, at 2,209,750 to 2,210,697
_RS10460
Position (kb)
2209
2210 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2208.532 kb on + strand, within BWI76_RS10445 at 2208.701 kb on - strand, within BWI76_RS10445 at 2208.741 kb on - strand, within BWI76_RS10445 at 2208.846 kb on + strand at 2208.854 kb on - strand at 2208.953 kb on + strand at 2208.953 kb on + strand at 2208.961 kb on - strand at 2208.961 kb on - strand at 2208.961 kb on - strand at 2208.963 kb on + strand at 2209.081 kb on - strand at 2209.112 kb on - strand, within BWI76_RS10450 at 2209.112 kb on - strand, within BWI76_RS10450 at 2209.145 kb on - strand at 2209.145 kb on - strand, within BWI76_RS10450 at 2209.152 kb on + strand, within BWI76_RS10450 at 2209.190 kb on + strand at 2209.237 kb on + strand at 2209.245 kb on - strand at 2209.326 kb on + strand at 2209.334 kb on - strand at 2209.337 kb on + strand at 2209.337 kb on + strand at 2209.337 kb on + strand at 2209.337 kb on + strand at 2209.345 kb on - strand at 2209.345 kb on - strand at 2209.345 kb on - strand at 2209.345 kb on - strand at 2209.347 kb on + strand at 2209.355 kb on - strand at 2209.395 kb on + strand at 2209.402 kb on - strand at 2209.445 kb on - strand at 2209.484 kb on + strand at 2209.492 kb on - strand at 2209.492 kb on - strand at 2209.492 kb on - strand at 2209.494 kb on + strand at 2209.494 kb on + strand at 2209.554 kb on + strand, within BWI76_RS10455 at 2209.562 kb on - strand, within BWI76_RS10455 at 2209.562 kb on - strand, within BWI76_RS10455 at 2209.573 kb on + strand at 2209.623 kb on + strand, within BWI76_RS10455 at 2209.632 kb on + strand, within BWI76_RS10455 at 2209.637 kb on + strand, within BWI76_RS10455 at 2209.657 kb on + strand, within BWI76_RS10455 at 2209.696 kb on + strand, within BWI76_RS10455 at 2209.696 kb on + strand, within BWI76_RS10455 at 2209.702 kb on + strand, within BWI76_RS10455 at 2209.702 kb on + strand, within BWI76_RS10455 at 2209.702 kb on + strand, within BWI76_RS10455 at 2209.703 kb on - strand, within BWI76_RS10455 at 2209.710 kb on - strand at 2209.710 kb on - strand at 2209.710 kb on - strand at 2209.718 kb on - strand at 2209.741 kb on + strand at 2209.744 kb on + strand at 2209.744 kb on + strand at 2209.774 kb on - strand at 2209.778 kb on + strand at 2209.778 kb on + strand at 2209.809 kb on + strand at 2209.820 kb on + strand at 2209.866 kb on - strand, within BWI76_RS10460 at 2209.869 kb on + strand, within BWI76_RS10460 at 2209.896 kb on + strand, within BWI76_RS10460 at 2209.898 kb on - strand, within BWI76_RS10460 at 2209.997 kb on + strand, within BWI76_RS10460 at 2210.088 kb on + strand, within BWI76_RS10460 at 2210.096 kb on - strand, within BWI76_RS10460 at 2210.171 kb on + strand, within BWI76_RS10460 at 2210.179 kb on - strand, within BWI76_RS10460 at 2210.226 kb on - strand, within BWI76_RS10460 at 2210.410 kb on + strand, within BWI76_RS10460 at 2210.425 kb on + strand, within BWI76_RS10460 at 2210.425 kb on + strand, within BWI76_RS10460 at 2210.435 kb on + strand, within BWI76_RS10460 at 2210.435 kb on + strand, within BWI76_RS10460 at 2210.443 kb on - strand, within BWI76_RS10460 at 2210.550 kb on - strand, within BWI76_RS10460 at 2210.567 kb on + strand, within BWI76_RS10460 at 2210.580 kb on + strand, within BWI76_RS10460 at 2210.612 kb on + strand at 2210.633 kb on - strand at 2210.638 kb on + strand at 2210.638 kb on + strand at 2210.646 kb on - strand at 2210.646 kb on - strand at 2210.646 kb on - strand at 2210.646 kb on - strand at 2210.655 kb on + strand at 2210.655 kb on + strand at 2210.663 kb on + strand at 2210.691 kb on + strand at 2210.696 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 2,208,532 + BWI76_RS10445 0.13 +0.1 2,208,701 - BWI76_RS10445 0.58 +0.9 2,208,741 - BWI76_RS10445 0.68 -0.4 2,208,846 + +0.8 2,208,854 - +0.8 2,208,953 + +0.7 2,208,953 + -0.3 2,208,961 - +0.3 2,208,961 - -0.5 2,208,961 - +0.7 2,208,963 + +1.0 2,209,081 - +0.1 2,209,112 - BWI76_RS10450 0.15 +1.0 2,209,112 - BWI76_RS10450 0.15 +0.1 2,209,145 - +2.1 2,209,145 - BWI76_RS10450 0.59 +0.8 2,209,152 + BWI76_RS10450 0.68 +1.8 2,209,190 + -3.6 2,209,237 + +0.6 2,209,245 - -2.1 2,209,326 + -1.1 2,209,334 - -1.2 2,209,337 + -3.2 2,209,337 + +0.6 2,209,337 + -2.1 2,209,337 + -3.3 2,209,345 - +1.4 2,209,345 - -2.0 2,209,345 - -0.7 2,209,345 - -1.8 2,209,347 + +1.7 2,209,355 - -1.8 2,209,395 + +1.2 2,209,402 - -0.4 2,209,445 - -0.4 2,209,484 + -0.0 2,209,492 - +0.5 2,209,492 - -1.1 2,209,492 - +0.5 2,209,494 + +0.4 2,209,494 + +0.2 2,209,554 + BWI76_RS10455 0.15 +1.3 2,209,562 - BWI76_RS10455 0.19 +2.2 2,209,562 - BWI76_RS10455 0.19 +2.7 2,209,573 + +3.1 2,209,623 + BWI76_RS10455 0.49 -0.3 2,209,632 + BWI76_RS10455 0.53 +1.2 2,209,637 + BWI76_RS10455 0.56 -0.9 2,209,657 + BWI76_RS10455 0.65 +0.0 2,209,696 + BWI76_RS10455 0.84 +0.4 2,209,696 + BWI76_RS10455 0.84 -4.2 2,209,702 + BWI76_RS10455 0.87 -0.0 2,209,702 + BWI76_RS10455 0.87 +0.7 2,209,702 + BWI76_RS10455 0.87 +0.5 2,209,703 - BWI76_RS10455 0.87 -0.0 2,209,710 - -1.0 2,209,710 - -0.7 2,209,710 - +0.1 2,209,718 - -2.2 2,209,741 + +0.4 2,209,744 + -0.8 2,209,744 + -2.4 2,209,774 - +1.0 2,209,778 + -1.1 2,209,778 + -3.2 2,209,809 + +1.6 2,209,820 + +1.2 2,209,866 - BWI76_RS10460 0.12 -2.2 2,209,869 + BWI76_RS10460 0.13 +1.7 2,209,896 + BWI76_RS10460 0.15 -0.2 2,209,898 - BWI76_RS10460 0.16 -3.2 2,209,997 + BWI76_RS10460 0.26 +0.1 2,210,088 + BWI76_RS10460 0.36 +0.3 2,210,096 - BWI76_RS10460 0.36 -0.7 2,210,171 + BWI76_RS10460 0.44 +1.2 2,210,179 - BWI76_RS10460 0.45 +0.5 2,210,226 - BWI76_RS10460 0.50 +1.6 2,210,410 + BWI76_RS10460 0.70 -0.4 2,210,425 + BWI76_RS10460 0.71 +1.1 2,210,425 + BWI76_RS10460 0.71 +0.5 2,210,435 + BWI76_RS10460 0.72 +0.3 2,210,435 + BWI76_RS10460 0.72 +0.1 2,210,443 - BWI76_RS10460 0.73 +1.9 2,210,550 - BWI76_RS10460 0.84 -0.2 2,210,567 + BWI76_RS10460 0.86 -2.4 2,210,580 + BWI76_RS10460 0.88 -0.8 2,210,612 + +0.7 2,210,633 - +1.4 2,210,638 + +0.4 2,210,638 + +0.8 2,210,646 - -1.6 2,210,646 - +1.4 2,210,646 - -1.3 2,210,646 - -0.7 2,210,655 + +1.4 2,210,655 + +1.2 2,210,663 + -3.0 2,210,691 + +0.1 2,210,696 + -2.4
Or see this region's nucleotide sequence