Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09595 and BWI76_RS09600 overlap by 4 nucleotides BWI76_RS09600 and BWI76_RS09605 are separated by 68 nucleotides BWI76_RS09605 and BWI76_RS09610 are separated by 324 nucleotides BWI76_RS09610 and BWI76_RS09615 are separated by 30 nucleotides
BWI76_RS09595: BWI76_RS09595 - macrolide transporter subunit MacA, at 2,007,481 to 2,008,596
_RS09595
BWI76_RS09600: BWI76_RS09600 - macrolide ABC transporter permease/ATP-binding protein MacB, at 2,008,593 to 2,010,539
_RS09600
BWI76_RS09605: BWI76_RS09605 - cold-shock protein, at 2,010,608 to 2,010,838
_RS09605
BWI76_RS09610: BWI76_RS09610 - ATP-dependent Clp protease adaptor ClpS, at 2,011,163 to 2,011,480
_RS09610
BWI76_RS09615: BWI76_RS09615 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 2,011,511 to 2,013,793
_RS09615
Position (kb)
2008
2009
2010
2011 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2007.616 kb on + strand, within BWI76_RS09595 at 2007.915 kb on - strand, within BWI76_RS09595 at 2008.219 kb on + strand, within BWI76_RS09595 at 2008.422 kb on + strand, within BWI76_RS09595 at 2008.669 kb on - strand at 2008.822 kb on + strand, within BWI76_RS09600 at 2009.085 kb on - strand, within BWI76_RS09600 at 2009.174 kb on - strand, within BWI76_RS09600 at 2009.179 kb on - strand, within BWI76_RS09600 at 2009.291 kb on - strand, within BWI76_RS09600 at 2009.342 kb on - strand, within BWI76_RS09600 at 2009.839 kb on + strand, within BWI76_RS09600 at 2009.990 kb on + strand, within BWI76_RS09600 at 2010.135 kb on + strand, within BWI76_RS09600 at 2010.151 kb on + strand, within BWI76_RS09600 at 2010.314 kb on - strand, within BWI76_RS09600 at 2010.584 kb on + strand at 2010.592 kb on - strand at 2010.832 kb on + strand at 2011.146 kb on - strand at 2011.165 kb on + strand at 2011.267 kb on - strand, within BWI76_RS09610 at 2011.337 kb on - strand, within BWI76_RS09610 at 2011.456 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 2,007,616 + BWI76_RS09595 0.12 +0.6 2,007,915 - BWI76_RS09595 0.39 -0.2 2,008,219 + BWI76_RS09595 0.66 -0.5 2,008,422 + BWI76_RS09595 0.84 -3.7 2,008,669 - -0.0 2,008,822 + BWI76_RS09600 0.12 +0.5 2,009,085 - BWI76_RS09600 0.25 -0.8 2,009,174 - BWI76_RS09600 0.30 -0.1 2,009,179 - BWI76_RS09600 0.30 +0.0 2,009,291 - BWI76_RS09600 0.36 +2.3 2,009,342 - BWI76_RS09600 0.38 +2.1 2,009,839 + BWI76_RS09600 0.64 +2.3 2,009,990 + BWI76_RS09600 0.72 +0.2 2,010,135 + BWI76_RS09600 0.79 +2.1 2,010,151 + BWI76_RS09600 0.80 +0.6 2,010,314 - BWI76_RS09600 0.88 +1.1 2,010,584 + +0.2 2,010,592 - -0.1 2,010,832 + +0.5 2,011,146 - +1.1 2,011,165 + -2.4 2,011,267 - BWI76_RS09610 0.33 -2.1 2,011,337 - BWI76_RS09610 0.55 +0.1 2,011,456 + -0.3
Or see this region's nucleotide sequence