Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09450 and BWI76_RS09455 are separated by 89 nucleotides BWI76_RS09455 and BWI76_RS09460 are separated by 349 nucleotides BWI76_RS09460 and BWI76_RS09465 are separated by 57 nucleotides
BWI76_RS09450: BWI76_RS09450 - 30S ribosomal protein S6--L-glutamate ligase, at 1,978,755 to 1,979,657
_RS09450
BWI76_RS09455: BWI76_RS09455 - YbjN domain-containing protein, at 1,979,747 to 1,980,226
_RS09455
BWI76_RS09460: BWI76_RS09460 - spermidine/putrescine ABC transporter substrate-binding protein PotF, at 1,980,576 to 1,981,688
_RS09460
BWI76_RS09465: BWI76_RS09465 - polyamine ABC transporter ATP-binding protein, at 1,981,746 to 1,982,924
_RS09465
Position (kb)
1980
1981
1982 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1979.932 kb on + strand, within BWI76_RS09455 at 1980.089 kb on - strand, within BWI76_RS09455 at 1980.110 kb on - strand, within BWI76_RS09455 at 1980.153 kb on - strand, within BWI76_RS09455 at 1980.174 kb on - strand, within BWI76_RS09455 at 1980.403 kb on - strand at 1980.419 kb on + strand at 1980.606 kb on + strand at 1980.611 kb on + strand at 1980.611 kb on + strand at 1980.619 kb on - strand at 1980.619 kb on - strand at 1980.675 kb on - strand at 1980.790 kb on - strand, within BWI76_RS09460 at 1980.816 kb on - strand, within BWI76_RS09460 at 1980.864 kb on - strand, within BWI76_RS09460 at 1981.028 kb on + strand, within BWI76_RS09460 at 1981.036 kb on - strand, within BWI76_RS09460 at 1981.504 kb on - strand, within BWI76_RS09460 at 1982.027 kb on - strand, within BWI76_RS09465 at 1982.245 kb on - strand, within BWI76_RS09465 at 1982.359 kb on - strand, within BWI76_RS09465 at 1982.535 kb on - strand, within BWI76_RS09465 at 1982.595 kb on + strand, within BWI76_RS09465 at 1982.603 kb on - strand, within BWI76_RS09465 at 1982.603 kb on - strand, within BWI76_RS09465 at 1982.613 kb on - strand, within BWI76_RS09465
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 1,979,932 + BWI76_RS09455 0.39 +1.3 1,980,089 - BWI76_RS09455 0.71 -0.7 1,980,110 - BWI76_RS09455 0.76 -0.4 1,980,153 - BWI76_RS09455 0.85 -0.7 1,980,174 - BWI76_RS09455 0.89 -1.6 1,980,403 - -0.0 1,980,419 + +2.1 1,980,606 + +0.7 1,980,611 + -0.9 1,980,611 + +0.3 1,980,619 - +0.5 1,980,619 - -0.0 1,980,675 - +0.4 1,980,790 - BWI76_RS09460 0.19 -1.8 1,980,816 - BWI76_RS09460 0.22 -1.9 1,980,864 - BWI76_RS09460 0.26 -1.0 1,981,028 + BWI76_RS09460 0.41 -0.7 1,981,036 - BWI76_RS09460 0.41 -0.2 1,981,504 - BWI76_RS09460 0.83 -2.1 1,982,027 - BWI76_RS09465 0.24 +0.5 1,982,245 - BWI76_RS09465 0.42 -1.0 1,982,359 - BWI76_RS09465 0.52 +0.5 1,982,535 - BWI76_RS09465 0.67 +0.5 1,982,595 + BWI76_RS09465 0.72 -2.1 1,982,603 - BWI76_RS09465 0.73 +2.2 1,982,603 - BWI76_RS09465 0.73 -0.0 1,982,613 - BWI76_RS09465 0.74 +0.6
Or see this region's nucleotide sequence