Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS07625

Experiment: KLEB17 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS07615 and BWI76_RS07620 are separated by 10 nucleotidesBWI76_RS07620 and BWI76_RS07625 overlap by 84 nucleotidesBWI76_RS07625 and BWI76_RS07630 overlap by 4 nucleotides BWI76_RS07615: BWI76_RS07615 - betaine-aldehyde dehydrogenase, at 1,613,138 to 1,614,610 _RS07615 BWI76_RS07620: BWI76_RS07620 - transcriptional regulator BetI, at 1,614,621 to 1,615,214 _RS07620 BWI76_RS07625: BWI76_RS07625 - hypothetical protein, at 1,615,131 to 1,615,346 _RS07625 BWI76_RS07630: BWI76_RS07630 - high-affinity choline transporter BetT, at 1,615,343 to 1,617,376 _RS07630 Position (kb) 1615 1616Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1614.458 kb on + strand, within BWI76_RS07615at 1614.501 kb on + strandat 1614.501 kb on + strandat 1614.605 kb on + strandat 1614.702 kb on + strand, within BWI76_RS07620at 1614.818 kb on + strand, within BWI76_RS07620at 1615.001 kb on - strand, within BWI76_RS07620at 1615.039 kb on + strand, within BWI76_RS07620at 1615.467 kb on + strandat 1615.478 kb on + strandat 1615.552 kb on + strand, within BWI76_RS07630at 1615.590 kb on + strandat 1615.730 kb on - strand, within BWI76_RS07630at 1616.014 kb on + strandat 1616.151 kb on + strand, within BWI76_RS07630at 1616.170 kb on + strand, within BWI76_RS07630at 1616.186 kb on - strand, within BWI76_RS07630

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB17 MOI 1
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1,614,458 + BWI76_RS07615 0.90 +0.8
1,614,501 + -3.1
1,614,501 + -1.1
1,614,605 + -1.3
1,614,702 + BWI76_RS07620 0.14 -0.6
1,614,818 + BWI76_RS07620 0.33 -0.2
1,615,001 - BWI76_RS07620 0.64 -1.4
1,615,039 + BWI76_RS07620 0.70 +0.2
1,615,467 + +3.7
1,615,478 + +1.8
1,615,552 + BWI76_RS07630 0.10 +0.2
1,615,590 + +0.9
1,615,730 - BWI76_RS07630 0.19 +0.2
1,616,014 + +3.2
1,616,151 + BWI76_RS07630 0.40 -3.0
1,616,170 + BWI76_RS07630 0.41 -0.5
1,616,186 - BWI76_RS07630 0.41 +0.3

Or see this region's nucleotide sequence