Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07250 and BWI76_RS07255 overlap by 4 nucleotides BWI76_RS07255 and BWI76_RS07260 are separated by 17 nucleotides BWI76_RS07260 and BWI76_RS07265 are separated by 27 nucleotides BWI76_RS07265 and BWI76_RS07270 overlap by 8 nucleotides
BWI76_RS07250: BWI76_RS07250 - Zn-dependent hydrolase, at 1,526,020 to 1,527,261
_RS07250
BWI76_RS07255: BWI76_RS07255 - hypothetical protein, at 1,527,258 to 1,527,740
_RS07255
BWI76_RS07260: BWI76_RS07260 - branched chain amino acid ABC transporter substrate-binding protein, at 1,527,758 to 1,528,915
_RS07260
BWI76_RS07265: BWI76_RS07265 - ABC transporter ATP-binding protein, at 1,528,943 to 1,529,662
_RS07265
BWI76_RS07270: BWI76_RS07270 - ABC transporter ATP-binding protein, at 1,529,655 to 1,530,422
_RS07270
Position (kb) 1527
1528
1529 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 1527.418 kb on - strand, within BWI76_RS07255 at 1527.480 kb on + strand, within BWI76_RS07255 at 1527.639 kb on + strand, within BWI76_RS07255 at 1527.761 kb on - strand at 1527.888 kb on - strand, within BWI76_RS07260 at 1528.023 kb on + strand, within BWI76_RS07260 at 1528.048 kb on - strand, within BWI76_RS07260 at 1528.503 kb on + strand, within BWI76_RS07260 at 1528.508 kb on + strand, within BWI76_RS07260 at 1528.970 kb on - strand at 1529.017 kb on + strand, within BWI76_RS07265 at 1529.220 kb on - strand, within BWI76_RS07265 at 1529.433 kb on - strand, within BWI76_RS07265 at 1529.539 kb on - strand, within BWI76_RS07265 at 1529.596 kb on - strand at 1529.596 kb on - strand at 1529.890 kb on + strand, within BWI76_RS07270
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 1,527,418 - BWI76_RS07255 0.33 +0.3 1,527,480 + BWI76_RS07255 0.46 +0.1 1,527,639 + BWI76_RS07255 0.79 -0.1 1,527,761 - +2.0 1,527,888 - BWI76_RS07260 0.11 +4.4 1,528,023 + BWI76_RS07260 0.23 +0.2 1,528,048 - BWI76_RS07260 0.25 -1.1 1,528,503 + BWI76_RS07260 0.64 +1.7 1,528,508 + BWI76_RS07260 0.65 +1.0 1,528,970 - +1.2 1,529,017 + BWI76_RS07265 0.10 -0.1 1,529,220 - BWI76_RS07265 0.38 +0.5 1,529,433 - BWI76_RS07265 0.68 +1.4 1,529,539 - BWI76_RS07265 0.83 +1.5 1,529,596 - -0.3 1,529,596 - -0.5 1,529,890 + BWI76_RS07270 0.31 -0.0
Or see this region's nucleotide sequence