Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS07255

Experiment: KLEB17 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS07250 and BWI76_RS07255 overlap by 4 nucleotidesBWI76_RS07255 and BWI76_RS07260 are separated by 17 nucleotides BWI76_RS07250: BWI76_RS07250 - Zn-dependent hydrolase, at 1,526,020 to 1,527,261 _RS07250 BWI76_RS07255: BWI76_RS07255 - hypothetical protein, at 1,527,258 to 1,527,740 _RS07255 BWI76_RS07260: BWI76_RS07260 - branched chain amino acid ABC transporter substrate-binding protein, at 1,527,758 to 1,528,915 _RS07260 Position (kb) 1527 1528Strain fitness (log2 ratio) -1 0 1 2 3 4at 1526.616 kb on + strand, within BWI76_RS07250at 1527.418 kb on - strand, within BWI76_RS07255at 1527.480 kb on + strand, within BWI76_RS07255at 1527.639 kb on + strand, within BWI76_RS07255at 1527.761 kb on - strandat 1527.888 kb on - strand, within BWI76_RS07260at 1528.023 kb on + strand, within BWI76_RS07260at 1528.048 kb on - strand, within BWI76_RS07260at 1528.503 kb on + strand, within BWI76_RS07260at 1528.508 kb on + strand, within BWI76_RS07260

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB17 MOI 1
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1,526,616 + BWI76_RS07250 0.48 -1.2
1,527,418 - BWI76_RS07255 0.33 +0.3
1,527,480 + BWI76_RS07255 0.46 +0.1
1,527,639 + BWI76_RS07255 0.79 -0.1
1,527,761 - +2.0
1,527,888 - BWI76_RS07260 0.11 +4.4
1,528,023 + BWI76_RS07260 0.23 +0.2
1,528,048 - BWI76_RS07260 0.25 -1.1
1,528,503 + BWI76_RS07260 0.64 +1.7
1,528,508 + BWI76_RS07260 0.65 +1.0

Or see this region's nucleotide sequence