Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06565 and BWI76_RS06570 are separated by 126 nucleotides BWI76_RS06570 and BWI76_RS06575 are separated by 47 nucleotides BWI76_RS06575 and BWI76_RS06580 are separated by 115 nucleotides BWI76_RS06580 and BWI76_RS06585 are separated by 42 nucleotides
BWI76_RS06565: BWI76_RS06565 - putative periplasmic solute-binding protein, at 1,379,614 to 1,381,314
_RS06565
BWI76_RS06570: BWI76_RS06570 - thiamin pyrimidine pyrophosphate hydrolase, at 1,381,441 to 1,382,259
_RS06570
BWI76_RS06575: BWI76_RS06575 - cysteine synthase, at 1,382,307 to 1,383,359
_RS06575
BWI76_RS06580: BWI76_RS06580 - transcriptional regulator, at 1,383,475 to 1,383,933
_RS06580
BWI76_RS06585: BWI76_RS06585 - multidrug ABC transporter permease/ATP-binding protein, at 1,383,976 to 1,385,748
_RS06585
Position (kb)
1382
1383
1384 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1381.317 kb on - strand at 1381.327 kb on - strand at 1381.672 kb on - strand, within BWI76_RS06570 at 1382.023 kb on + strand, within BWI76_RS06570 at 1382.751 kb on - strand, within BWI76_RS06575 at 1382.751 kb on - strand, within BWI76_RS06575 at 1382.869 kb on + strand, within BWI76_RS06575 at 1382.917 kb on - strand, within BWI76_RS06575 at 1382.926 kb on - strand, within BWI76_RS06575 at 1383.246 kb on + strand, within BWI76_RS06575 at 1383.398 kb on - strand at 1383.562 kb on - strand, within BWI76_RS06580 at 1383.567 kb on - strand, within BWI76_RS06580 at 1383.689 kb on - strand, within BWI76_RS06580 at 1383.964 kb on + strand at 1384.045 kb on - strand at 1384.241 kb on + strand, within BWI76_RS06585
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 1,381,317 - +0.4 1,381,327 - -0.6 1,381,672 - BWI76_RS06570 0.28 -1.0 1,382,023 + BWI76_RS06570 0.71 +0.1 1,382,751 - BWI76_RS06575 0.42 -0.7 1,382,751 - BWI76_RS06575 0.42 +0.2 1,382,869 + BWI76_RS06575 0.53 -0.9 1,382,917 - BWI76_RS06575 0.58 -0.1 1,382,926 - BWI76_RS06575 0.59 -0.6 1,383,246 + BWI76_RS06575 0.89 -0.4 1,383,398 - +1.4 1,383,562 - BWI76_RS06580 0.19 +2.6 1,383,567 - BWI76_RS06580 0.20 +4.1 1,383,689 - BWI76_RS06580 0.47 +0.5 1,383,964 + +0.6 1,384,045 - -1.2 1,384,241 + BWI76_RS06585 0.15 -0.5
Or see this region's nucleotide sequence