Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03180 and BWI76_RS03185 overlap by 4 nucleotides BWI76_RS03185 and BWI76_RS03190 overlap by 4 nucleotides BWI76_RS03190 and BWI76_RS03195 overlap by 4 nucleotides BWI76_RS03195 and BWI76_RS03200 are separated by 11 nucleotides
BWI76_RS03180: BWI76_RS03180 - phage transcriptional regulator, AlpA, at 656,346 to 656,612
_RS03180
BWI76_RS03185: BWI76_RS03185 - CI repressor, at 656,609 to 657,166
_RS03185
BWI76_RS03190: BWI76_RS03190 - hypothetical protein, at 657,163 to 657,390
_RS03190
BWI76_RS03195: BWI76_RS03195 - P4 phage protein, at 657,387 to 657,707
_RS03195
BWI76_RS03200: BWI76_RS03200 - nucleoside triphosphatase, D5 family protein, at 657,719 to 660,052
_RS03200
Position (kb) 657
658 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 656.292 kb on + strand at 656.402 kb on + strand, within BWI76_RS03180 at 656.402 kb on + strand, within BWI76_RS03180 at 656.410 kb on - strand, within BWI76_RS03180 at 656.784 kb on - strand, within BWI76_RS03185 at 656.802 kb on + strand, within BWI76_RS03185 at 656.868 kb on - strand, within BWI76_RS03185 at 656.960 kb on - strand, within BWI76_RS03185 at 656.994 kb on - strand, within BWI76_RS03185 at 657.258 kb on + strand, within BWI76_RS03190 at 657.260 kb on + strand, within BWI76_RS03190 at 657.330 kb on + strand, within BWI76_RS03190 at 657.416 kb on - strand at 657.435 kb on + strand, within BWI76_RS03195 at 657.453 kb on - strand, within BWI76_RS03195 at 657.476 kb on - strand, within BWI76_RS03195 at 657.884 kb on - strand at 657.931 kb on + strand at 658.035 kb on - strand, within BWI76_RS03200 at 658.107 kb on + strand, within BWI76_RS03200 at 658.294 kb on + strand, within BWI76_RS03200 at 658.302 kb on - strand, within BWI76_RS03200
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 656,292 + +1.9 656,402 + BWI76_RS03180 0.21 +1.2 656,402 + BWI76_RS03180 0.21 -0.1 656,410 - BWI76_RS03180 0.24 -1.9 656,784 - BWI76_RS03185 0.31 -1.6 656,802 + BWI76_RS03185 0.35 +0.2 656,868 - BWI76_RS03185 0.46 -0.2 656,960 - BWI76_RS03185 0.63 -1.5 656,994 - BWI76_RS03185 0.69 -0.9 657,258 + BWI76_RS03190 0.42 +0.8 657,260 + BWI76_RS03190 0.43 +0.8 657,330 + BWI76_RS03190 0.73 +1.4 657,416 - -0.6 657,435 + BWI76_RS03195 0.15 +0.1 657,453 - BWI76_RS03195 0.21 +0.8 657,476 - BWI76_RS03195 0.28 -0.3 657,884 - +0.2 657,931 + +0.0 658,035 - BWI76_RS03200 0.14 -3.8 658,107 + BWI76_RS03200 0.17 -0.1 658,294 + BWI76_RS03200 0.25 -0.9 658,302 - BWI76_RS03200 0.25 +1.7
Or see this region's nucleotide sequence