Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01350 and BWI76_RS01355 are separated by 44 nucleotides BWI76_RS01355 and BWI76_RS01360 are separated by 126 nucleotides BWI76_RS01360 and BWI76_RS01365 are separated by 9 nucleotides
BWI76_RS01350: BWI76_RS01350 - NAD(P)H-flavin reductase, at 276,280 to 276,981
_RS01350
BWI76_RS01355: BWI76_RS01355 - hypothetical protein, at 277,026 to 277,337
_RS01355
BWI76_RS01360: BWI76_RS01360 - acetyl-CoA C-acyltransferase FadA, at 277,464 to 278,627
_RS01360
BWI76_RS01365: BWI76_RS01365 - multifunctional fatty acid oxidation complex subunit alpha, at 278,637 to 280,826
_RS01365
Position (kb) 277
278
279 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 276.740 kb on + strand, within BWI76_RS01350 at 277.128 kb on - strand, within BWI76_RS01355 at 277.128 kb on - strand, within BWI76_RS01355 at 277.174 kb on + strand, within BWI76_RS01355 at 277.174 kb on + strand, within BWI76_RS01355 at 277.182 kb on - strand, within BWI76_RS01355 at 277.213 kb on + strand, within BWI76_RS01355 at 277.221 kb on - strand, within BWI76_RS01355 at 277.252 kb on + strand, within BWI76_RS01355 at 277.277 kb on - strand at 277.306 kb on - strand at 277.344 kb on + strand at 277.426 kb on - strand at 277.458 kb on - strand at 277.470 kb on - strand at 277.497 kb on + strand at 277.545 kb on - strand at 277.545 kb on - strand at 277.562 kb on + strand at 277.602 kb on + strand, within BWI76_RS01360 at 277.602 kb on + strand, within BWI76_RS01360 at 277.652 kb on - strand, within BWI76_RS01360 at 277.814 kb on - strand, within BWI76_RS01360 at 277.907 kb on + strand, within BWI76_RS01360 at 277.933 kb on + strand, within BWI76_RS01360 at 278.006 kb on + strand, within BWI76_RS01360 at 278.022 kb on + strand, within BWI76_RS01360 at 278.103 kb on - strand, within BWI76_RS01360 at 278.137 kb on - strand, within BWI76_RS01360 at 278.446 kb on - strand, within BWI76_RS01360 at 278.446 kb on - strand, within BWI76_RS01360 at 278.446 kb on - strand, within BWI76_RS01360 at 278.803 kb on + strand at 278.894 kb on + strand, within BWI76_RS01365 at 278.979 kb on + strand, within BWI76_RS01365 at 279.102 kb on - strand, within BWI76_RS01365 at 279.293 kb on + strand, within BWI76_RS01365 at 279.475 kb on - strand, within BWI76_RS01365 at 279.514 kb on - strand, within BWI76_RS01365 at 279.546 kb on + strand, within BWI76_RS01365
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 276,740 + BWI76_RS01350 0.66 -3.0 277,128 - BWI76_RS01355 0.33 +0.1 277,128 - BWI76_RS01355 0.33 -0.3 277,174 + BWI76_RS01355 0.47 +1.9 277,174 + BWI76_RS01355 0.47 -1.5 277,182 - BWI76_RS01355 0.50 +0.2 277,213 + BWI76_RS01355 0.60 +0.0 277,221 - BWI76_RS01355 0.62 +0.5 277,252 + BWI76_RS01355 0.72 +0.3 277,277 - +1.5 277,306 - +1.0 277,344 + +0.2 277,426 - +0.4 277,458 - +1.6 277,470 - -0.0 277,497 + -0.8 277,545 - -0.2 277,545 - +1.0 277,562 + -0.3 277,602 + BWI76_RS01360 0.12 -0.9 277,602 + BWI76_RS01360 0.12 -1.1 277,652 - BWI76_RS01360 0.16 -0.4 277,814 - BWI76_RS01360 0.30 -3.4 277,907 + BWI76_RS01360 0.38 +0.5 277,933 + BWI76_RS01360 0.40 -0.3 278,006 + BWI76_RS01360 0.47 +0.4 278,022 + BWI76_RS01360 0.48 -0.4 278,103 - BWI76_RS01360 0.55 +0.5 278,137 - BWI76_RS01360 0.58 -0.7 278,446 - BWI76_RS01360 0.84 -0.9 278,446 - BWI76_RS01360 0.84 -0.8 278,446 - BWI76_RS01360 0.84 +0.3 278,803 + -4.3 278,894 + BWI76_RS01365 0.12 -0.7 278,979 + BWI76_RS01365 0.16 +1.3 279,102 - BWI76_RS01365 0.21 +1.4 279,293 + BWI76_RS01365 0.30 +0.2 279,475 - BWI76_RS01365 0.38 -0.1 279,514 - BWI76_RS01365 0.40 -0.4 279,546 + BWI76_RS01365 0.42 +0.3
Or see this region's nucleotide sequence