Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01060

Experiment: KLEB17 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01055 and BWI76_RS01060 are separated by 18 nucleotidesBWI76_RS01060 and BWI76_RS01065 are separated by 134 nucleotides BWI76_RS01055: BWI76_RS01055 - LysR family transcriptional regulator, at 215,873 to 216,793 _RS01055 BWI76_RS01060: BWI76_RS01060 - guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase, at 216,812 to 218,311 _RS01060 BWI76_RS01065: BWI76_RS01065 - ATP-dependent RNA helicase RhlB, at 218,446 to 219,711 _RS01065 Position (kb) 216 217 218 219Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 215.819 kb on + strandat 215.825 kb on - strandat 215.825 kb on - strandat 215.886 kb on + strandat 215.888 kb on + strandat 215.888 kb on + strandat 215.894 kb on - strandat 215.896 kb on - strandat 215.897 kb on + strandat 215.901 kb on + strandat 215.903 kb on + strandat 215.916 kb on - strandat 215.923 kb on + strandat 215.923 kb on + strandat 215.931 kb on - strandat 215.931 kb on - strandat 215.931 kb on - strandat 215.933 kb on + strandat 215.933 kb on + strandat 215.933 kb on + strandat 215.941 kb on - strandat 215.941 kb on - strandat 215.941 kb on - strandat 215.941 kb on - strandat 215.951 kb on + strandat 215.953 kb on + strandat 215.958 kb on + strandat 215.964 kb on + strandat 215.964 kb on + strandat 215.964 kb on + strandat 215.972 kb on - strand, within BWI76_RS01055at 215.972 kb on - strand, within BWI76_RS01055at 215.977 kb on - strand, within BWI76_RS01055at 216.009 kb on + strand, within BWI76_RS01055at 216.017 kb on + strand, within BWI76_RS01055at 216.036 kb on + strand, within BWI76_RS01055at 216.044 kb on - strand, within BWI76_RS01055at 216.073 kb on + strand, within BWI76_RS01055at 216.113 kb on + strand, within BWI76_RS01055at 216.121 kb on + strand, within BWI76_RS01055at 216.121 kb on + strand, within BWI76_RS01055at 216.121 kb on + strand, within BWI76_RS01055at 216.122 kb on + strand, within BWI76_RS01055at 216.129 kb on - strand, within BWI76_RS01055at 216.129 kb on - strand, within BWI76_RS01055at 216.129 kb on - strand, within BWI76_RS01055at 216.129 kb on - strand, within BWI76_RS01055at 216.129 kb on - strand, within BWI76_RS01055at 216.129 kb on - strand, within BWI76_RS01055at 216.130 kb on - strand, within BWI76_RS01055at 216.130 kb on - strand, within BWI76_RS01055at 216.176 kb on + strand, within BWI76_RS01055at 216.181 kb on + strand, within BWI76_RS01055at 216.184 kb on - strand, within BWI76_RS01055at 216.184 kb on - strand, within BWI76_RS01055at 216.184 kb on - strand, within BWI76_RS01055at 216.184 kb on - strand, within BWI76_RS01055at 216.189 kb on + strand, within BWI76_RS01055at 216.191 kb on - strand, within BWI76_RS01055at 216.227 kb on + strand, within BWI76_RS01055at 216.335 kb on - strand, within BWI76_RS01055at 216.367 kb on + strand, within BWI76_RS01055at 216.390 kb on - strand, within BWI76_RS01055at 216.445 kb on - strand, within BWI76_RS01055at 216.743 kb on + strandat 216.869 kb on - strandat 217.032 kb on + strand, within BWI76_RS01060at 217.032 kb on + strand, within BWI76_RS01060at 217.227 kb on - strand, within BWI76_RS01060at 217.430 kb on - strand, within BWI76_RS01060at 217.434 kb on - strand, within BWI76_RS01060at 217.435 kb on - strand, within BWI76_RS01060at 217.442 kb on - strand, within BWI76_RS01060at 217.717 kb on + strand, within BWI76_RS01060at 217.720 kb on - strand, within BWI76_RS01060at 217.835 kb on - strand, within BWI76_RS01060at 217.993 kb on - strand, within BWI76_RS01060at 218.297 kb on - strandat 218.299 kb on + strandat 218.321 kb on - strandat 218.332 kb on + strandat 218.340 kb on - strandat 218.448 kb on + strandat 218.451 kb on - strandat 218.533 kb on - strandat 218.533 kb on - strandat 218.965 kb on + strand, within BWI76_RS01065at 218.966 kb on + strand, within BWI76_RS01065at 219.065 kb on + strand, within BWI76_RS01065at 219.124 kb on - strand, within BWI76_RS01065at 219.124 kb on - strand, within BWI76_RS01065at 219.254 kb on - strand, within BWI76_RS01065

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB17 MOI 1
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215,819 + +2.1
215,825 - -0.1
215,825 - -3.3
215,886 + -1.1
215,888 + -0.5
215,888 + -0.4
215,894 - -1.4
215,896 - +0.4
215,897 + -4.1
215,901 + -0.3
215,903 + +0.3
215,916 - -0.5
215,923 + +1.5
215,923 + +1.1
215,931 - -0.5
215,931 - +4.1
215,931 - -0.1
215,933 + -0.4
215,933 + -1.3
215,933 + -2.3
215,941 - -2.3
215,941 - +0.5
215,941 - -0.6
215,941 - -0.2
215,951 + +0.5
215,953 + -0.1
215,958 + +1.1
215,964 + -2.8
215,964 + +0.5
215,964 + +0.8
215,972 - BWI76_RS01055 0.11 +1.0
215,972 - BWI76_RS01055 0.11 -3.7
215,977 - BWI76_RS01055 0.11 -0.9
216,009 + BWI76_RS01055 0.15 +2.3
216,017 + BWI76_RS01055 0.16 -1.8
216,036 + BWI76_RS01055 0.18 -2.0
216,044 - BWI76_RS01055 0.19 -0.4
216,073 + BWI76_RS01055 0.22 -3.1
216,113 + BWI76_RS01055 0.26 +1.2
216,121 + BWI76_RS01055 0.27 +1.9
216,121 + BWI76_RS01055 0.27 -1.9
216,121 + BWI76_RS01055 0.27 +0.7
216,122 + BWI76_RS01055 0.27 -1.4
216,129 - BWI76_RS01055 0.28 -3.4
216,129 - BWI76_RS01055 0.28 +0.8
216,129 - BWI76_RS01055 0.28 +1.4
216,129 - BWI76_RS01055 0.28 +0.1
216,129 - BWI76_RS01055 0.28 -0.1
216,129 - BWI76_RS01055 0.28 -0.4
216,130 - BWI76_RS01055 0.28 -0.7
216,130 - BWI76_RS01055 0.28 +1.5
216,176 + BWI76_RS01055 0.33 +0.4
216,181 + BWI76_RS01055 0.33 -1.1
216,184 - BWI76_RS01055 0.34 +1.0
216,184 - BWI76_RS01055 0.34 +1.5
216,184 - BWI76_RS01055 0.34 -1.1
216,184 - BWI76_RS01055 0.34 -2.1
216,189 + BWI76_RS01055 0.34 +0.3
216,191 - BWI76_RS01055 0.35 +1.7
216,227 + BWI76_RS01055 0.38 +0.6
216,335 - BWI76_RS01055 0.50 +0.6
216,367 + BWI76_RS01055 0.54 -3.1
216,390 - BWI76_RS01055 0.56 -0.1
216,445 - BWI76_RS01055 0.62 -1.9
216,743 + +0.7
216,869 - -0.4
217,032 + BWI76_RS01060 0.15 -0.8
217,032 + BWI76_RS01060 0.15 -1.1
217,227 - BWI76_RS01060 0.28 -0.8
217,430 - BWI76_RS01060 0.41 -4.4
217,434 - BWI76_RS01060 0.41 +1.1
217,435 - BWI76_RS01060 0.42 -0.8
217,442 - BWI76_RS01060 0.42 -1.9
217,717 + BWI76_RS01060 0.60 -2.7
217,720 - BWI76_RS01060 0.61 +2.1
217,835 - BWI76_RS01060 0.68 -0.4
217,993 - BWI76_RS01060 0.79 -0.3
218,297 - +1.5
218,299 + +0.2
218,321 - -0.9
218,332 + -0.4
218,340 - +1.7
218,448 + -2.1
218,451 - +0.6
218,533 - -0.4
218,533 - -2.1
218,965 + BWI76_RS01065 0.41 +0.5
218,966 + BWI76_RS01065 0.41 -3.8
219,065 + BWI76_RS01065 0.49 -2.5
219,124 - BWI76_RS01065 0.54 -2.9
219,124 - BWI76_RS01065 0.54 -3.4
219,254 - BWI76_RS01065 0.64 -1.6

Or see this region's nucleotide sequence