Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25200 and BWI76_RS25205 are separated by 54 nucleotides BWI76_RS25205 and BWI76_RS25210 are separated by 135 nucleotides BWI76_RS25210 and BWI76_RS25215 overlap by 1 nucleotides BWI76_RS25215 and BWI76_RS25220 are separated by 18 nucleotides BWI76_RS25220 and BWI76_RS25225 are separated by 4 nucleotides
BWI76_RS25200: BWI76_RS25200 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 5,242,406 to 5,243,665
_RS25200
BWI76_RS25205: BWI76_RS25205 - hypothetical protein, at 5,243,720 to 5,243,974
_RS25205
BWI76_RS25210: BWI76_RS25210 - sulfate transporter, at 5,244,110 to 5,244,400
_RS25210
BWI76_RS25215: BWI76_RS25215 - phospholipid-binding protein MlaC, at 5,244,400 to 5,245,035
_RS25215
BWI76_RS25220: BWI76_RS25220 - outer membrane lipid asymmetry maintenance protein MlaD, at 5,245,054 to 5,245,605
_RS25220
BWI76_RS25225: BWI76_RS25225 - ABC transporter permease, at 5,245,610 to 5,246,392
_RS25225
Position (kb) 5244
5245
5246 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6
7 at 5243.761 kb on - strand, within BWI76_RS25205 at 5244.001 kb on - strand at 5244.019 kb on - strand at 5244.039 kb on - strand at 5244.176 kb on + strand, within BWI76_RS25210 at 5244.381 kb on + strand at 5244.440 kb on + strand at 5244.624 kb on + strand, within BWI76_RS25215 at 5244.666 kb on - strand, within BWI76_RS25215 at 5244.677 kb on - strand, within BWI76_RS25215 at 5244.737 kb on - strand, within BWI76_RS25215 at 5244.890 kb on + strand, within BWI76_RS25215 at 5244.956 kb on - strand, within BWI76_RS25215 at 5245.147 kb on + strand, within BWI76_RS25220 at 5245.147 kb on - strand, within BWI76_RS25220 at 5245.224 kb on + strand, within BWI76_RS25220 at 5245.268 kb on + strand, within BWI76_RS25220 at 5245.326 kb on + strand, within BWI76_RS25220 at 5245.369 kb on - strand, within BWI76_RS25220 at 5245.572 kb on + strand at 5246.024 kb on + strand, within BWI76_RS25225
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 5,243,761 - BWI76_RS25205 0.16 +0.3 5,244,001 - -3.2 5,244,019 - +0.2 5,244,039 - +0.1 5,244,176 + BWI76_RS25210 0.23 -2.7 5,244,381 + -0.3 5,244,440 + -2.6 5,244,624 + BWI76_RS25215 0.35 -1.0 5,244,666 - BWI76_RS25215 0.42 -0.9 5,244,677 - BWI76_RS25215 0.44 -0.8 5,244,737 - BWI76_RS25215 0.53 -2.2 5,244,890 + BWI76_RS25215 0.77 -3.9 5,244,956 - BWI76_RS25215 0.87 -2.2 5,245,147 + BWI76_RS25220 0.17 -2.7 5,245,147 - BWI76_RS25220 0.17 +6.9 5,245,224 + BWI76_RS25220 0.31 +0.7 5,245,268 + BWI76_RS25220 0.39 -1.9 5,245,326 + BWI76_RS25220 0.49 -0.8 5,245,369 - BWI76_RS25220 0.57 +5.0 5,245,572 + -0.1 5,246,024 + BWI76_RS25225 0.53 -1.2
Or see this region's nucleotide sequence