Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24820 and BWI76_RS24825 are separated by 65 nucleotides BWI76_RS24825 and BWI76_RS24830 are separated by 28 nucleotides BWI76_RS24830 and BWI76_RS24835 are separated by 28 nucleotides
BWI76_RS24820: BWI76_RS24820 - glycerate 2-kinase, at 5,171,423 to 5,172,568
_RS24820
BWI76_RS24825: BWI76_RS24825 - 2-hydroxy-3-oxopropionate reductase, at 5,172,634 to 5,173,524
_RS24825
BWI76_RS24830: BWI76_RS24830 - 5-keto-4-deoxy-D-glucarate aldolase, at 5,173,553 to 5,174,323
_RS24830
BWI76_RS24835: BWI76_RS24835 - MFS transporter, at 5,174,352 to 5,175,686
_RS24835
Position (kb) 5172
5173
5174 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5172.133 kb on - strand, within BWI76_RS24820 at 5172.248 kb on - strand, within BWI76_RS24820 at 5172.257 kb on + strand, within BWI76_RS24820 at 5172.271 kb on + strand, within BWI76_RS24820 at 5172.394 kb on - strand, within BWI76_RS24820 at 5172.511 kb on - strand at 5172.535 kb on + strand at 5172.604 kb on - strand at 5172.610 kb on - strand at 5172.627 kb on + strand at 5172.650 kb on + strand at 5172.892 kb on - strand, within BWI76_RS24825 at 5173.072 kb on - strand, within BWI76_RS24825 at 5173.533 kb on - strand at 5173.636 kb on - strand, within BWI76_RS24830 at 5173.639 kb on + strand, within BWI76_RS24830 at 5173.741 kb on + strand, within BWI76_RS24830 at 5173.777 kb on - strand, within BWI76_RS24830 at 5173.864 kb on - strand, within BWI76_RS24830 at 5173.923 kb on + strand, within BWI76_RS24830 at 5173.923 kb on + strand, within BWI76_RS24830 at 5174.191 kb on - strand, within BWI76_RS24830 at 5174.326 kb on + strand at 5174.334 kb on - strand at 5174.412 kb on + strand at 5174.446 kb on + strand at 5174.487 kb on - strand, within BWI76_RS24835
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 5,172,133 - BWI76_RS24820 0.62 +0.8 5,172,248 - BWI76_RS24820 0.72 +1.2 5,172,257 + BWI76_RS24820 0.73 -0.1 5,172,271 + BWI76_RS24820 0.74 +2.3 5,172,394 - BWI76_RS24820 0.85 +2.1 5,172,511 - -2.9 5,172,535 + +0.4 5,172,604 - -1.9 5,172,610 - -0.7 5,172,627 + +0.4 5,172,650 + +0.5 5,172,892 - BWI76_RS24825 0.29 +1.0 5,173,072 - BWI76_RS24825 0.49 -0.8 5,173,533 - -1.5 5,173,636 - BWI76_RS24830 0.11 -1.5 5,173,639 + BWI76_RS24830 0.11 -2.9 5,173,741 + BWI76_RS24830 0.24 +0.5 5,173,777 - BWI76_RS24830 0.29 -0.3 5,173,864 - BWI76_RS24830 0.40 -1.7 5,173,923 + BWI76_RS24830 0.48 -1.6 5,173,923 + BWI76_RS24830 0.48 -2.0 5,174,191 - BWI76_RS24830 0.83 -0.6 5,174,326 + +0.1 5,174,334 - -2.8 5,174,412 + -0.1 5,174,446 + -0.8 5,174,487 - BWI76_RS24835 0.10 +0.9
Or see this region's nucleotide sequence