Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23965

Experiment: KLEB17 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23955 and BWI76_RS23960 are separated by 121 nucleotidesBWI76_RS23960 and BWI76_RS23965 are separated by 90 nucleotidesBWI76_RS23965 and BWI76_RS23970 are separated by 150 nucleotides BWI76_RS23955: BWI76_RS23955 - LysR family transcriptional regulator, at 5,000,363 to 5,001,259 _RS23955 BWI76_RS23960: BWI76_RS23960 - oxidative stress defense protein, at 5,001,381 to 5,002,148 _RS23960 BWI76_RS23965: BWI76_RS23965 - arginine exporter protein, at 5,002,239 to 5,002,874 _RS23965 BWI76_RS23970: BWI76_RS23970 - mechanosensitive ion channel protein MscS, at 5,003,025 to 5,003,882 _RS23970 Position (kb) 5002 5003Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 5001.474 kb on - strand, within BWI76_RS23960at 5001.603 kb on - strand, within BWI76_RS23960at 5001.644 kb on - strand, within BWI76_RS23960at 5001.702 kb on - strand, within BWI76_RS23960at 5001.769 kb on + strand, within BWI76_RS23960at 5001.873 kb on - strand, within BWI76_RS23960at 5001.936 kb on - strand, within BWI76_RS23960at 5002.184 kb on - strandat 5002.252 kb on + strandat 5002.355 kb on + strand, within BWI76_RS23965at 5002.435 kb on - strand, within BWI76_RS23965at 5003.009 kb on + strandat 5003.220 kb on - strand, within BWI76_RS23970at 5003.415 kb on + strand, within BWI76_RS23970at 5003.415 kb on + strand, within BWI76_RS23970at 5003.538 kb on - strand, within BWI76_RS23970at 5003.800 kb on + strandat 5003.813 kb on - strandat 5003.834 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB17 MOI 1
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5,001,474 - BWI76_RS23960 0.12 -4.2
5,001,603 - BWI76_RS23960 0.29 +0.2
5,001,644 - BWI76_RS23960 0.34 +0.3
5,001,702 - BWI76_RS23960 0.42 -1.3
5,001,769 + BWI76_RS23960 0.51 +0.5
5,001,873 - BWI76_RS23960 0.64 +1.4
5,001,936 - BWI76_RS23960 0.72 +0.7
5,002,184 - -0.0
5,002,252 + +0.4
5,002,355 + BWI76_RS23965 0.18 -0.9
5,002,435 - BWI76_RS23965 0.31 -1.8
5,003,009 + -1.0
5,003,220 - BWI76_RS23970 0.23 +1.8
5,003,415 + BWI76_RS23970 0.45 +0.4
5,003,415 + BWI76_RS23970 0.45 +0.7
5,003,538 - BWI76_RS23970 0.60 -2.1
5,003,800 + +0.2
5,003,813 - -2.8
5,003,834 + -0.4

Or see this region's nucleotide sequence