Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23935 and BWI76_RS23940 overlap by 8 nucleotides BWI76_RS23940 and BWI76_RS23945 are separated by 10 nucleotides BWI76_RS23945 and BWI76_RS23950 are separated by 24 nucleotides
BWI76_RS23935: BWI76_RS23935 - methylmalonyl-CoA mutase, at 4,994,947 to 4,997,091
_RS23935
BWI76_RS23940: BWI76_RS23940 - ATPase/protein kinase, at 4,997,084 to 4,998,079
_RS23940
BWI76_RS23945: BWI76_RS23945 - methylmalonyl-CoA decarboxylase, at 4,998,090 to 4,998,875
_RS23945
BWI76_RS23950: BWI76_RS23950 - succinate CoA transferase, at 4,998,900 to 5,000,366
_RS23950
Position (kb) 4997
4998
4999 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 4996.117 kb on - strand, within BWI76_RS23935 at 4996.176 kb on + strand, within BWI76_RS23935 at 4996.298 kb on - strand, within BWI76_RS23935 at 4996.367 kb on + strand, within BWI76_RS23935 at 4996.375 kb on - strand, within BWI76_RS23935 at 4996.454 kb on + strand, within BWI76_RS23935 at 4996.528 kb on - strand, within BWI76_RS23935 at 4996.587 kb on + strand, within BWI76_RS23935 at 4996.781 kb on + strand, within BWI76_RS23935 at 4996.795 kb on - strand, within BWI76_RS23935 at 4996.909 kb on - strand at 4997.038 kb on + strand at 4997.148 kb on - strand at 4997.426 kb on + strand, within BWI76_RS23940 at 4997.519 kb on + strand, within BWI76_RS23940 at 4997.519 kb on + strand, within BWI76_RS23940 at 4997.701 kb on - strand, within BWI76_RS23940 at 4997.701 kb on - strand, within BWI76_RS23940 at 4997.947 kb on + strand, within BWI76_RS23940 at 4998.334 kb on - strand, within BWI76_RS23945 at 4998.407 kb on + strand, within BWI76_RS23945 at 4998.806 kb on - strand at 4998.891 kb on - strand at 4998.891 kb on - strand at 4998.891 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 4,996,117 - BWI76_RS23935 0.55 +1.0 4,996,176 + BWI76_RS23935 0.57 +2.0 4,996,298 - BWI76_RS23935 0.63 -1.0 4,996,367 + BWI76_RS23935 0.66 +0.7 4,996,375 - BWI76_RS23935 0.67 +2.5 4,996,454 + BWI76_RS23935 0.70 +0.1 4,996,528 - BWI76_RS23935 0.74 +0.1 4,996,587 + BWI76_RS23935 0.76 +0.4 4,996,781 + BWI76_RS23935 0.86 +4.8 4,996,795 - BWI76_RS23935 0.86 -0.1 4,996,909 - -0.4 4,997,038 + -0.7 4,997,148 - +0.4 4,997,426 + BWI76_RS23940 0.34 -1.6 4,997,519 + BWI76_RS23940 0.44 +0.0 4,997,519 + BWI76_RS23940 0.44 +0.8 4,997,701 - BWI76_RS23940 0.62 +0.6 4,997,701 - BWI76_RS23940 0.62 -1.5 4,997,947 + BWI76_RS23940 0.87 +0.7 4,998,334 - BWI76_RS23945 0.31 -0.2 4,998,407 + BWI76_RS23945 0.40 +0.7 4,998,806 - -3.1 4,998,891 - +0.9 4,998,891 - +0.1 4,998,891 - +0.2
Or see this region's nucleotide sequence