Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23925 and BWI76_RS23930 are separated by 169 nucleotides BWI76_RS23930 and BWI76_RS23935 are separated by 198 nucleotides
BWI76_RS23925: BWI76_RS23925 - ribose-5-phosphate isomerase, at 4,993,026 to 4,993,685
_RS23925
BWI76_RS23930: BWI76_RS23930 - transcriptional regulator ArgP, at 4,993,855 to 4,994,748
_RS23930
BWI76_RS23935: BWI76_RS23935 - methylmalonyl-CoA mutase, at 4,994,947 to 4,997,091
_RS23935
Position (kb)
4993
4994
4995 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4993.048 kb on + strand at 4993.057 kb on + strand at 4993.407 kb on + strand, within BWI76_RS23925 at 4993.628 kb on + strand at 4993.845 kb on + strand at 4993.877 kb on - strand at 4993.885 kb on - strand at 4993.904 kb on + strand at 4994.015 kb on + strand, within BWI76_RS23930 at 4994.269 kb on + strand, within BWI76_RS23930 at 4994.288 kb on + strand, within BWI76_RS23930 at 4994.357 kb on + strand, within BWI76_RS23930 at 4994.402 kb on + strand, within BWI76_RS23930 at 4994.750 kb on - strand at 4995.167 kb on - strand, within BWI76_RS23935 at 4995.167 kb on - strand, within BWI76_RS23935 at 4995.167 kb on - strand, within BWI76_RS23935 at 4995.167 kb on - strand, within BWI76_RS23935 at 4995.212 kb on - strand, within BWI76_RS23935 at 4995.259 kb on + strand, within BWI76_RS23935 at 4995.419 kb on + strand, within BWI76_RS23935 at 4995.476 kb on + strand, within BWI76_RS23935 at 4995.525 kb on + strand, within BWI76_RS23935 at 4995.631 kb on - strand, within BWI76_RS23935 at 4995.668 kb on - strand, within BWI76_RS23935
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 4,993,048 + -0.4 4,993,057 + +2.0 4,993,407 + BWI76_RS23925 0.58 +1.2 4,993,628 + -0.8 4,993,845 + +1.1 4,993,877 - -1.6 4,993,885 - +0.2 4,993,904 + -1.6 4,994,015 + BWI76_RS23930 0.18 -0.7 4,994,269 + BWI76_RS23930 0.46 -0.8 4,994,288 + BWI76_RS23930 0.48 -4.6 4,994,357 + BWI76_RS23930 0.56 -0.5 4,994,402 + BWI76_RS23930 0.61 -1.2 4,994,750 - -2.8 4,995,167 - BWI76_RS23935 0.10 -0.6 4,995,167 - BWI76_RS23935 0.10 -0.2 4,995,167 - BWI76_RS23935 0.10 +0.7 4,995,167 - BWI76_RS23935 0.10 +1.0 4,995,212 - BWI76_RS23935 0.12 +0.5 4,995,259 + BWI76_RS23935 0.15 +0.8 4,995,419 + BWI76_RS23935 0.22 +0.7 4,995,476 + BWI76_RS23935 0.25 -0.5 4,995,525 + BWI76_RS23935 0.27 -0.5 4,995,631 - BWI76_RS23935 0.32 -0.0 4,995,668 - BWI76_RS23935 0.34 +0.4
Or see this region's nucleotide sequence