Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23380

Experiment: KLEB17 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23370 and BWI76_RS23375 are separated by 135 nucleotidesBWI76_RS23375 and BWI76_RS23380 are separated by 493 nucleotidesBWI76_RS23380 and BWI76_RS23385 overlap by 8 nucleotidesBWI76_RS23385 and BWI76_RS23390 are separated by 12 nucleotides BWI76_RS23370: BWI76_RS23370 - aspartate/glutamate racemase, at 4,873,650 to 4,874,342 _RS23370 BWI76_RS23375: BWI76_RS23375 - cupin, at 4,874,478 to 4,874,804 _RS23375 BWI76_RS23380: BWI76_RS23380 - sugar ABC transporter permease, at 4,875,298 to 4,876,188 _RS23380 BWI76_RS23385: BWI76_RS23385 - sugar ABC transporter permease, at 4,876,181 to 4,877,083 _RS23385 BWI76_RS23390: BWI76_RS23390 - oligogalacturonide ABC transporter ATP-binding protein, at 4,877,096 to 4,878,223 _RS23390 Position (kb) 4875 4876 4877Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 4874.311 kb on - strandat 4874.519 kb on - strand, within BWI76_RS23375at 4874.519 kb on - strand, within BWI76_RS23375at 4874.578 kb on + strand, within BWI76_RS23375at 4874.627 kb on + strand, within BWI76_RS23375at 4874.627 kb on + strand, within BWI76_RS23375at 4874.632 kb on + strand, within BWI76_RS23375at 4874.632 kb on + strand, within BWI76_RS23375at 4874.640 kb on - strand, within BWI76_RS23375at 4874.821 kb on - strandat 4874.821 kb on - strandat 4874.941 kb on + strandat 4874.950 kb on - strandat 4874.961 kb on + strandat 4874.961 kb on + strandat 4874.961 kb on + strandat 4874.969 kb on - strandat 4874.969 kb on - strandat 4874.969 kb on - strandat 4875.256 kb on + strandat 4875.287 kb on + strandat 4875.404 kb on - strand, within BWI76_RS23380at 4875.430 kb on - strand, within BWI76_RS23380at 4875.430 kb on - strand, within BWI76_RS23380at 4875.457 kb on - strand, within BWI76_RS23380at 4875.497 kb on - strand, within BWI76_RS23380at 4875.580 kb on - strand, within BWI76_RS23380at 4875.580 kb on - strand, within BWI76_RS23380at 4875.580 kb on - strand, within BWI76_RS23380at 4875.580 kb on - strand, within BWI76_RS23380at 4875.580 kb on - strand, within BWI76_RS23380at 4875.580 kb on - strand, within BWI76_RS23380at 4875.580 kb on - strand, within BWI76_RS23380at 4875.702 kb on - strand, within BWI76_RS23380at 4875.721 kb on - strand, within BWI76_RS23380at 4875.739 kb on - strand, within BWI76_RS23380at 4875.769 kb on - strand, within BWI76_RS23380at 4876.020 kb on + strand, within BWI76_RS23380at 4876.047 kb on - strandat 4876.086 kb on - strand, within BWI76_RS23380at 4876.086 kb on - strand, within BWI76_RS23380at 4876.233 kb on - strandat 4876.310 kb on - strand, within BWI76_RS23385at 4876.322 kb on + strand, within BWI76_RS23385at 4876.371 kb on + strand, within BWI76_RS23385at 4876.444 kb on - strand, within BWI76_RS23385at 4876.610 kb on - strand, within BWI76_RS23385at 4876.613 kb on - strand, within BWI76_RS23385at 4876.694 kb on + strand, within BWI76_RS23385at 4876.798 kb on + strand, within BWI76_RS23385at 4877.043 kb on + strandat 4877.168 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB17 MOI 1
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4,874,311 - -1.4
4,874,519 - BWI76_RS23375 0.13 +0.2
4,874,519 - BWI76_RS23375 0.13 -1.8
4,874,578 + BWI76_RS23375 0.31 -1.3
4,874,627 + BWI76_RS23375 0.46 -1.7
4,874,627 + BWI76_RS23375 0.46 -0.8
4,874,632 + BWI76_RS23375 0.47 +2.3
4,874,632 + BWI76_RS23375 0.47 -1.6
4,874,640 - BWI76_RS23375 0.50 +1.3
4,874,821 - -0.5
4,874,821 - -0.5
4,874,941 + -5.1
4,874,950 - +2.7
4,874,961 + -0.8
4,874,961 + -0.5
4,874,961 + +0.8
4,874,969 - +2.3
4,874,969 - -1.0
4,874,969 - -0.1
4,875,256 + -2.3
4,875,287 + -0.1
4,875,404 - BWI76_RS23380 0.12 +0.3
4,875,430 - BWI76_RS23380 0.15 -0.0
4,875,430 - BWI76_RS23380 0.15 +1.2
4,875,457 - BWI76_RS23380 0.18 -1.3
4,875,497 - BWI76_RS23380 0.22 -0.7
4,875,580 - BWI76_RS23380 0.32 +0.9
4,875,580 - BWI76_RS23380 0.32 -0.8
4,875,580 - BWI76_RS23380 0.32 -0.4
4,875,580 - BWI76_RS23380 0.32 -0.4
4,875,580 - BWI76_RS23380 0.32 -1.3
4,875,580 - BWI76_RS23380 0.32 -0.3
4,875,580 - BWI76_RS23380 0.32 -2.4
4,875,702 - BWI76_RS23380 0.45 +1.0
4,875,721 - BWI76_RS23380 0.47 +0.0
4,875,739 - BWI76_RS23380 0.49 -2.6
4,875,769 - BWI76_RS23380 0.53 -2.7
4,876,020 + BWI76_RS23380 0.81 -0.9
4,876,047 - +1.8
4,876,086 - BWI76_RS23380 0.88 -0.8
4,876,086 - BWI76_RS23380 0.88 +0.1
4,876,233 - -0.1
4,876,310 - BWI76_RS23385 0.14 -0.8
4,876,322 + BWI76_RS23385 0.16 +0.4
4,876,371 + BWI76_RS23385 0.21 +0.2
4,876,444 - BWI76_RS23385 0.29 -0.1
4,876,610 - BWI76_RS23385 0.48 +0.6
4,876,613 - BWI76_RS23385 0.48 -0.7
4,876,694 + BWI76_RS23385 0.57 +0.4
4,876,798 + BWI76_RS23385 0.68 -1.4
4,877,043 + +0.9
4,877,168 - +0.4

Or see this region's nucleotide sequence