Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22100

Experiment: KLEB17 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22095 and BWI76_RS22100 are separated by 15 nucleotidesBWI76_RS22100 and BWI76_RS22105 are separated by 143 nucleotides BWI76_RS22095: BWI76_RS22095 - multidrug export protein EmrA, at 4,611,814 to 4,612,986 _RS22095 BWI76_RS22100: BWI76_RS22100 - multidrug effux MFS transporter subunit EmrB, at 4,613,002 to 4,614,540 _RS22100 BWI76_RS22105: BWI76_RS22105 - 2-isopropylmalate synthase, at 4,614,684 to 4,616,360 _RS22105 Position (kb) 4613 4614 4615Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4612.093 kb on - strandat 4612.204 kb on - strand, within BWI76_RS22095at 4612.390 kb on - strand, within BWI76_RS22095at 4612.997 kb on + strandat 4613.131 kb on - strandat 4613.221 kb on - strand, within BWI76_RS22100at 4613.901 kb on - strand, within BWI76_RS22100at 4614.041 kb on + strand, within BWI76_RS22100at 4614.109 kb on + strand, within BWI76_RS22100at 4614.287 kb on + strand, within BWI76_RS22100at 4614.608 kb on + strandat 4614.689 kb on + strandat 4615.021 kb on - strand, within BWI76_RS22105at 4615.343 kb on + strand, within BWI76_RS22105at 4615.344 kb on - strand, within BWI76_RS22105at 4615.344 kb on - strand, within BWI76_RS22105at 4615.351 kb on - strand, within BWI76_RS22105at 4615.351 kb on - strand, within BWI76_RS22105

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB17 MOI 1
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4,612,093 - -1.3
4,612,204 - BWI76_RS22095 0.33 -0.4
4,612,390 - BWI76_RS22095 0.49 +0.4
4,612,997 + -1.1
4,613,131 - +1.9
4,613,221 - BWI76_RS22100 0.14 -3.5
4,613,901 - BWI76_RS22100 0.58 -2.1
4,614,041 + BWI76_RS22100 0.68 -1.2
4,614,109 + BWI76_RS22100 0.72 -1.2
4,614,287 + BWI76_RS22100 0.83 -0.1
4,614,608 + -0.6
4,614,689 + -1.8
4,615,021 - BWI76_RS22105 0.20 +0.9
4,615,343 + BWI76_RS22105 0.39 -4.5
4,615,344 - BWI76_RS22105 0.39 +0.1
4,615,344 - BWI76_RS22105 0.39 -0.8
4,615,351 - BWI76_RS22105 0.40 -0.1
4,615,351 - BWI76_RS22105 0.40 -1.0

Or see this region's nucleotide sequence