Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21990 and BWI76_RS21995 are separated by 149 nucleotides BWI76_RS21995 and BWI76_RS22000 are separated by 9 nucleotides BWI76_RS22000 and BWI76_RS22005 are separated by 91 nucleotides BWI76_RS22005 and BWI76_RS22010 are separated by 46 nucleotides
BWI76_RS21990: BWI76_RS21990 - ATPase AAA, at 4,594,709 to 4,596,493
_RS21990
BWI76_RS21995: BWI76_RS21995 - transcriptional regulator, at 4,596,643 to 4,596,942
_RS21995
BWI76_RS22000: BWI76_RS22000 - hypothetical protein, at 4,596,952 to 4,597,473
_RS22000
BWI76_RS22005: BWI76_RS22005 - hypothetical protein, at 4,597,565 to 4,597,744
_RS22005
BWI76_RS22010: BWI76_RS22010 - DNA binding protein, nucleoid-associated, at 4,597,791 to 4,598,192
_RS22010
Position (kb)
4596
4597 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4595.649 kb on - strand, within BWI76_RS21990 at 4595.693 kb on + strand, within BWI76_RS21990 at 4595.758 kb on - strand, within BWI76_RS21990 at 4595.824 kb on - strand, within BWI76_RS21990 at 4595.831 kb on + strand, within BWI76_RS21990 at 4595.942 kb on - strand, within BWI76_RS21990 at 4596.059 kb on + strand, within BWI76_RS21990 at 4596.086 kb on + strand, within BWI76_RS21990 at 4596.205 kb on - strand, within BWI76_RS21990 at 4596.225 kb on - strand, within BWI76_RS21990 at 4596.362 kb on - strand at 4596.438 kb on - strand at 4596.453 kb on + strand at 4596.585 kb on - strand at 4596.666 kb on + strand at 4596.708 kb on - strand, within BWI76_RS21995 at 4596.787 kb on + strand, within BWI76_RS21995 at 4596.857 kb on + strand, within BWI76_RS21995 at 4596.944 kb on - strand at 4596.956 kb on - strand at 4597.115 kb on + strand, within BWI76_RS22000 at 4597.164 kb on + strand, within BWI76_RS22000 at 4597.164 kb on + strand, within BWI76_RS22000 at 4597.164 kb on + strand, within BWI76_RS22000 at 4597.172 kb on - strand, within BWI76_RS22000 at 4597.172 kb on - strand, within BWI76_RS22000 at 4597.318 kb on + strand, within BWI76_RS22000 at 4597.339 kb on + strand, within BWI76_RS22000 at 4597.404 kb on - strand, within BWI76_RS22000 at 4597.475 kb on - strand at 4597.475 kb on - strand at 4597.493 kb on + strand at 4597.501 kb on - strand at 4597.524 kb on - strand at 4597.547 kb on + strand at 4597.547 kb on + strand at 4597.547 kb on + strand at 4597.555 kb on - strand at 4597.567 kb on + strand at 4597.584 kb on + strand, within BWI76_RS22005 at 4597.588 kb on - strand, within BWI76_RS22005 at 4597.612 kb on + strand, within BWI76_RS22005 at 4597.613 kb on - strand, within BWI76_RS22005 at 4597.620 kb on - strand, within BWI76_RS22005 at 4597.627 kb on + strand, within BWI76_RS22005 at 4597.642 kb on + strand, within BWI76_RS22005 at 4597.650 kb on - strand, within BWI76_RS22005 at 4597.657 kb on + strand, within BWI76_RS22005 at 4597.662 kb on + strand, within BWI76_RS22005 at 4597.681 kb on - strand, within BWI76_RS22005 at 4597.743 kb on + strand at 4597.743 kb on + strand at 4597.751 kb on - strand at 4597.751 kb on - strand at 4597.766 kb on - strand at 4597.766 kb on - strand at 4597.774 kb on + strand at 4597.797 kb on - strand at 4597.850 kb on + strand, within BWI76_RS22010 at 4597.850 kb on + strand, within BWI76_RS22010 at 4597.852 kb on - strand, within BWI76_RS22010 at 4597.857 kb on + strand, within BWI76_RS22010 at 4597.857 kb on + strand, within BWI76_RS22010 at 4597.857 kb on + strand, within BWI76_RS22010 at 4597.858 kb on - strand, within BWI76_RS22010 at 4597.865 kb on - strand, within BWI76_RS22010 at 4597.865 kb on - strand, within BWI76_RS22010 at 4597.865 kb on - strand, within BWI76_RS22010 at 4597.867 kb on + strand, within BWI76_RS22010 at 4597.880 kb on - strand, within BWI76_RS22010 at 4597.890 kb on - strand, within BWI76_RS22010 at 4597.920 kb on + strand, within BWI76_RS22010
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 4,595,649 - BWI76_RS21990 0.53 -0.8 4,595,693 + BWI76_RS21990 0.55 -3.4 4,595,758 - BWI76_RS21990 0.59 -0.8 4,595,824 - BWI76_RS21990 0.62 -0.1 4,595,831 + BWI76_RS21990 0.63 +1.4 4,595,942 - BWI76_RS21990 0.69 -0.1 4,596,059 + BWI76_RS21990 0.76 +0.9 4,596,086 + BWI76_RS21990 0.77 -0.1 4,596,205 - BWI76_RS21990 0.84 +0.8 4,596,225 - BWI76_RS21990 0.85 +0.2 4,596,362 - +0.5 4,596,438 - -0.8 4,596,453 + -0.3 4,596,585 - +0.7 4,596,666 + +0.7 4,596,708 - BWI76_RS21995 0.22 -1.5 4,596,787 + BWI76_RS21995 0.48 -0.5 4,596,857 + BWI76_RS21995 0.71 -1.7 4,596,944 - -3.9 4,596,956 - +0.7 4,597,115 + BWI76_RS22000 0.31 +0.2 4,597,164 + BWI76_RS22000 0.41 +0.3 4,597,164 + BWI76_RS22000 0.41 +1.2 4,597,164 + BWI76_RS22000 0.41 -0.8 4,597,172 - BWI76_RS22000 0.42 +0.9 4,597,172 - BWI76_RS22000 0.42 -0.7 4,597,318 + BWI76_RS22000 0.70 -3.4 4,597,339 + BWI76_RS22000 0.74 -2.2 4,597,404 - BWI76_RS22000 0.87 -1.5 4,597,475 - -0.3 4,597,475 - +1.7 4,597,493 + -1.2 4,597,501 - +0.4 4,597,524 - -0.3 4,597,547 + +0.6 4,597,547 + -1.4 4,597,547 + +0.8 4,597,555 - -0.3 4,597,567 + +1.9 4,597,584 + BWI76_RS22005 0.11 -0.5 4,597,588 - BWI76_RS22005 0.13 +0.2 4,597,612 + BWI76_RS22005 0.26 +0.1 4,597,613 - BWI76_RS22005 0.27 -0.3 4,597,620 - BWI76_RS22005 0.31 -0.9 4,597,627 + BWI76_RS22005 0.34 -0.5 4,597,642 + BWI76_RS22005 0.43 -2.7 4,597,650 - BWI76_RS22005 0.47 +1.9 4,597,657 + BWI76_RS22005 0.51 -0.5 4,597,662 + BWI76_RS22005 0.54 -1.1 4,597,681 - BWI76_RS22005 0.64 -0.8 4,597,743 + +0.7 4,597,743 + +1.0 4,597,751 - +0.6 4,597,751 - +1.2 4,597,766 - -1.1 4,597,766 - -2.4 4,597,774 + -0.1 4,597,797 - +0.1 4,597,850 + BWI76_RS22010 0.15 -1.6 4,597,850 + BWI76_RS22010 0.15 +0.4 4,597,852 - BWI76_RS22010 0.15 -0.7 4,597,857 + BWI76_RS22010 0.16 -2.6 4,597,857 + BWI76_RS22010 0.16 +2.2 4,597,857 + BWI76_RS22010 0.16 +0.9 4,597,858 - BWI76_RS22010 0.17 -3.3 4,597,865 - BWI76_RS22010 0.18 -1.0 4,597,865 - BWI76_RS22010 0.18 +1.3 4,597,865 - BWI76_RS22010 0.18 -0.7 4,597,867 + BWI76_RS22010 0.19 -2.0 4,597,880 - BWI76_RS22010 0.22 -1.5 4,597,890 - BWI76_RS22010 0.25 -1.8 4,597,920 + BWI76_RS22010 0.32 -1.1
Or see this region's nucleotide sequence