Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18895 and BWI76_RS18900 overlap by 1 nucleotides BWI76_RS18900 and BWI76_RS18905 are separated by 74 nucleotides BWI76_RS18905 and BWI76_RS18910 are separated by 287 nucleotides
BWI76_RS18895: BWI76_RS18895 - nitrogenase cofactor biosynthesis protein NifB, at 3,918,844 to 3,920,250
_RS18895
BWI76_RS18900: BWI76_RS18900 - protein NifQ, at 3,920,250 to 3,920,753
_RS18900
BWI76_RS18905: BWI76_RS18905 - putrescine/spermidine ABC transporter, at 3,920,828 to 3,922,186
_RS18905
BWI76_RS18910: BWI76_RS18910 - LysR family transcriptional regulator, at 3,922,474 to 3,923,364
_RS18910
Position (kb)
3920
3921
3922
3923 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3920.014 kb on - strand, within BWI76_RS18895 at 3920.081 kb on - strand, within BWI76_RS18895 at 3920.334 kb on - strand, within BWI76_RS18900 at 3920.451 kb on + strand, within BWI76_RS18900 at 3920.565 kb on - strand, within BWI76_RS18900 at 3920.846 kb on - strand at 3921.562 kb on - strand, within BWI76_RS18905 at 3921.880 kb on + strand, within BWI76_RS18905 at 3921.903 kb on - strand, within BWI76_RS18905 at 3922.283 kb on + strand at 3922.490 kb on + strand at 3922.503 kb on + strand at 3922.586 kb on + strand, within BWI76_RS18910 at 3922.673 kb on + strand, within BWI76_RS18910 at 3922.681 kb on - strand, within BWI76_RS18910 at 3922.683 kb on + strand, within BWI76_RS18910 at 3922.696 kb on - strand, within BWI76_RS18910 at 3922.899 kb on + strand, within BWI76_RS18910 at 3923.095 kb on + strand, within BWI76_RS18910
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 3,920,014 - BWI76_RS18895 0.83 -0.5 3,920,081 - BWI76_RS18895 0.88 +0.4 3,920,334 - BWI76_RS18900 0.17 +0.3 3,920,451 + BWI76_RS18900 0.40 +0.7 3,920,565 - BWI76_RS18900 0.62 +0.4 3,920,846 - -3.2 3,921,562 - BWI76_RS18905 0.54 -2.5 3,921,880 + BWI76_RS18905 0.77 +1.9 3,921,903 - BWI76_RS18905 0.79 -0.6 3,922,283 + -0.4 3,922,490 + -1.2 3,922,503 + +1.1 3,922,586 + BWI76_RS18910 0.13 +3.7 3,922,673 + BWI76_RS18910 0.22 +0.8 3,922,681 - BWI76_RS18910 0.23 +0.3 3,922,683 + BWI76_RS18910 0.23 +0.1 3,922,696 - BWI76_RS18910 0.25 -1.3 3,922,899 + BWI76_RS18910 0.48 +1.0 3,923,095 + BWI76_RS18910 0.70 +0.5
Or see this region's nucleotide sequence