Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18310 and BWI76_RS18315 are separated by 13 nucleotides BWI76_RS18315 and BWI76_RS18320 are separated by 38 nucleotides BWI76_RS18320 and BWI76_RS18325 are separated by 151 nucleotides BWI76_RS18325 and BWI76_RS18330 are separated by 57 nucleotides BWI76_RS18330 and BWI76_RS18335 overlap by 4 nucleotides
BWI76_RS18310: BWI76_RS18310 - tRNA-Leu, at 3,804,531 to 3,804,617
_RS18310
BWI76_RS18315: BWI76_RS18315 - tRNA-Cys, at 3,804,631 to 3,804,704
_RS18315
BWI76_RS18320: BWI76_RS18320 - tRNA-Gly, at 3,804,743 to 3,804,818
_RS18320
BWI76_RS18325: BWI76_RS18325 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 3,804,970 to 3,805,518
_RS18325
BWI76_RS18330: BWI76_RS18330 - excinuclease ABC subunit C, at 3,805,576 to 3,807,408
_RS18330
BWI76_RS18335: BWI76_RS18335 - DNA-binding response regulator, at 3,807,405 to 3,808,061
_RS18335
Position (kb)
3805
3806
3807
3808 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3805.581 kb on + strand at 3805.828 kb on - strand, within BWI76_RS18330 at 3805.859 kb on - strand, within BWI76_RS18330 at 3805.859 kb on - strand, within BWI76_RS18330 at 3806.088 kb on + strand, within BWI76_RS18330 at 3806.828 kb on - strand, within BWI76_RS18330 at 3806.868 kb on - strand, within BWI76_RS18330 at 3806.880 kb on + strand, within BWI76_RS18330 at 3806.880 kb on + strand, within BWI76_RS18330 at 3806.888 kb on - strand, within BWI76_RS18330 at 3806.888 kb on - strand, within BWI76_RS18330 at 3806.888 kb on - strand, within BWI76_RS18330 at 3806.888 kb on - strand, within BWI76_RS18330 at 3806.888 kb on - strand, within BWI76_RS18330 at 3806.987 kb on - strand, within BWI76_RS18330 at 3807.005 kb on + strand, within BWI76_RS18330 at 3807.432 kb on + strand at 3807.435 kb on - strand at 3807.440 kb on - strand at 3807.467 kb on + strand at 3807.472 kb on - strand, within BWI76_RS18335 at 3807.653 kb on + strand, within BWI76_RS18335 at 3807.696 kb on - strand, within BWI76_RS18335 at 3807.902 kb on - strand, within BWI76_RS18335 at 3807.922 kb on - strand, within BWI76_RS18335 at 3807.933 kb on + strand, within BWI76_RS18335 at 3807.963 kb on - strand, within BWI76_RS18335 at 3807.966 kb on - strand, within BWI76_RS18335 at 3807.971 kb on - strand, within BWI76_RS18335 at 3807.977 kb on + strand, within BWI76_RS18335 at 3807.977 kb on + strand, within BWI76_RS18335 at 3807.985 kb on - strand, within BWI76_RS18335 at 3807.986 kb on + strand, within BWI76_RS18335 at 3808.008 kb on + strand at 3808.016 kb on - strand at 3808.016 kb on - strand at 3808.018 kb on - strand at 3808.035 kb on - strand at 3808.045 kb on + strand at 3808.056 kb on + strand at 3808.056 kb on + strand at 3808.064 kb on - strand at 3808.064 kb on - strand at 3808.084 kb on + strand at 3808.093 kb on + strand at 3808.101 kb on - strand at 3808.200 kb on - strand at 3808.200 kb on - strand at 3808.219 kb on - strand at 3808.220 kb on + strand at 3808.220 kb on + strand at 3808.220 kb on + strand at 3808.225 kb on + strand at 3808.228 kb on - strand at 3808.228 kb on - strand at 3808.228 kb on - strand at 3808.228 kb on - strand at 3808.228 kb on - strand at 3808.228 kb on - strand at 3808.233 kb on - strand at 3808.276 kb on - strand at 3808.276 kb on - strand at 3808.283 kb on - strand at 3808.283 kb on - strand at 3808.283 kb on - strand at 3808.292 kb on + strand at 3808.295 kb on - strand at 3808.300 kb on - strand at 3808.300 kb on - strand at 3808.300 kb on - strand at 3808.302 kb on - strand at 3808.338 kb on - strand at 3808.360 kb on + strand at 3808.361 kb on + strand at 3808.361 kb on + strand at 3808.361 kb on + strand at 3808.361 kb on + strand at 3808.361 kb on + strand at 3808.369 kb on - strand at 3808.369 kb on - strand at 3808.369 kb on - strand at 3808.369 kb on - strand at 3808.369 kb on - strand at 3808.369 kb on - strand at 3808.369 kb on - strand at 3808.369 kb on - strand at 3808.380 kb on + strand at 3808.388 kb on - strand at 3808.407 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 3,805,581 + +0.6 3,805,828 - BWI76_RS18330 0.14 -0.1 3,805,859 - BWI76_RS18330 0.15 -0.6 3,805,859 - BWI76_RS18330 0.15 +0.6 3,806,088 + BWI76_RS18330 0.28 -2.4 3,806,828 - BWI76_RS18330 0.68 +0.4 3,806,868 - BWI76_RS18330 0.70 -1.6 3,806,880 + BWI76_RS18330 0.71 -0.9 3,806,880 + BWI76_RS18330 0.71 +0.1 3,806,888 - BWI76_RS18330 0.72 +1.7 3,806,888 - BWI76_RS18330 0.72 +0.1 3,806,888 - BWI76_RS18330 0.72 +3.6 3,806,888 - BWI76_RS18330 0.72 +0.7 3,806,888 - BWI76_RS18330 0.72 -0.4 3,806,987 - BWI76_RS18330 0.77 +1.6 3,807,005 + BWI76_RS18330 0.78 -0.1 3,807,432 + +2.5 3,807,435 - +2.0 3,807,440 - +1.5 3,807,467 + +1.1 3,807,472 - BWI76_RS18335 0.10 +2.1 3,807,653 + BWI76_RS18335 0.38 +1.7 3,807,696 - BWI76_RS18335 0.44 +1.5 3,807,902 - BWI76_RS18335 0.76 +0.4 3,807,922 - BWI76_RS18335 0.79 +1.9 3,807,933 + BWI76_RS18335 0.80 +1.6 3,807,963 - BWI76_RS18335 0.85 -0.1 3,807,966 - BWI76_RS18335 0.85 +2.1 3,807,971 - BWI76_RS18335 0.86 +2.0 3,807,977 + BWI76_RS18335 0.87 +0.4 3,807,977 + BWI76_RS18335 0.87 +1.6 3,807,985 - BWI76_RS18335 0.88 +1.1 3,807,986 + BWI76_RS18335 0.88 -1.1 3,808,008 + -0.6 3,808,016 - +1.5 3,808,016 - -0.8 3,808,018 - +1.3 3,808,035 - +1.5 3,808,045 + +1.9 3,808,056 + -1.3 3,808,056 + +2.7 3,808,064 - +0.2 3,808,064 - +2.4 3,808,084 + +0.0 3,808,093 + -0.8 3,808,101 - +1.0 3,808,200 - +1.5 3,808,200 - +0.5 3,808,219 - -3.2 3,808,220 + -0.5 3,808,220 + +0.5 3,808,220 + -2.4 3,808,225 + -2.2 3,808,228 - -0.9 3,808,228 - -0.0 3,808,228 - +1.0 3,808,228 - -0.5 3,808,228 - +0.2 3,808,228 - -2.4 3,808,233 - -2.9 3,808,276 - -2.4 3,808,276 - -0.6 3,808,283 - -0.5 3,808,283 - +0.6 3,808,283 - +0.7 3,808,292 + -3.3 3,808,295 - +0.7 3,808,300 - -0.5 3,808,300 - -3.8 3,808,300 - -3.2 3,808,302 - -1.2 3,808,338 - -3.2 3,808,360 + -2.7 3,808,361 + -0.3 3,808,361 + -1.1 3,808,361 + -1.2 3,808,361 + -1.8 3,808,361 + -2.7 3,808,369 - -1.0 3,808,369 - +2.0 3,808,369 - -1.9 3,808,369 - -2.9 3,808,369 - -2.2 3,808,369 - -3.4 3,808,369 - +1.2 3,808,369 - -0.5 3,808,380 + -1.4 3,808,388 - -0.9 3,808,407 + +0.6
Or see this region's nucleotide sequence