Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17815 and BWI76_RS17820 are separated by 132 nucleotides BWI76_RS17820 and BWI76_RS17825 are separated by 164 nucleotides BWI76_RS17825 and BWI76_RS17830 are separated by 7 nucleotides
BWI76_RS17815: BWI76_RS17815 - ATP-dependent helicase, at 3,702,414 to 3,704,324
_RS17815
BWI76_RS17820: BWI76_RS17820 - hypothetical protein, at 3,704,457 to 3,704,801
_RS17820
BWI76_RS17825: BWI76_RS17825 - alginate lyase, at 3,704,966 to 3,706,105
_RS17825
BWI76_RS17830: BWI76_RS17830 - ABC transporter ATP-binding protein, at 3,706,113 to 3,707,207
_RS17830
Position (kb)
3704
3705
3706
3707 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3703.980 kb on - strand, within BWI76_RS17815 at 3703.993 kb on - strand, within BWI76_RS17815 at 3703.993 kb on - strand, within BWI76_RS17815 at 3704.266 kb on + strand at 3704.530 kb on - strand, within BWI76_RS17820 at 3704.724 kb on + strand, within BWI76_RS17820 at 3704.796 kb on + strand at 3704.796 kb on + strand at 3704.804 kb on - strand at 3704.804 kb on - strand at 3704.828 kb on - strand at 3705.108 kb on + strand, within BWI76_RS17825 at 3705.136 kb on - strand, within BWI76_RS17825 at 3705.520 kb on + strand, within BWI76_RS17825 at 3705.763 kb on - strand, within BWI76_RS17825 at 3705.780 kb on - strand, within BWI76_RS17825 at 3705.861 kb on + strand, within BWI76_RS17825 at 3705.939 kb on - strand, within BWI76_RS17825 at 3706.011 kb on + strand at 3706.035 kb on + strand at 3706.272 kb on - strand, within BWI76_RS17830 at 3706.345 kb on + strand, within BWI76_RS17830 at 3706.353 kb on - strand, within BWI76_RS17830 at 3706.355 kb on + strand, within BWI76_RS17830 at 3706.377 kb on - strand, within BWI76_RS17830 at 3706.647 kb on - strand, within BWI76_RS17830 at 3706.665 kb on + strand, within BWI76_RS17830 at 3706.885 kb on + strand, within BWI76_RS17830 at 3706.913 kb on - strand, within BWI76_RS17830 at 3706.996 kb on - strand, within BWI76_RS17830
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 3,703,980 - BWI76_RS17815 0.82 +0.6 3,703,993 - BWI76_RS17815 0.83 -2.6 3,703,993 - BWI76_RS17815 0.83 -1.1 3,704,266 + +0.8 3,704,530 - BWI76_RS17820 0.21 -1.2 3,704,724 + BWI76_RS17820 0.77 -2.9 3,704,796 + +0.8 3,704,796 + +0.1 3,704,804 - -1.7 3,704,804 - -0.3 3,704,828 - -0.5 3,705,108 + BWI76_RS17825 0.12 +0.5 3,705,136 - BWI76_RS17825 0.15 +0.2 3,705,520 + BWI76_RS17825 0.49 -3.7 3,705,763 - BWI76_RS17825 0.70 +1.4 3,705,780 - BWI76_RS17825 0.71 -0.5 3,705,861 + BWI76_RS17825 0.79 +2.0 3,705,939 - BWI76_RS17825 0.85 -0.3 3,706,011 + +2.3 3,706,035 + +0.9 3,706,272 - BWI76_RS17830 0.15 +1.5 3,706,345 + BWI76_RS17830 0.21 +2.8 3,706,353 - BWI76_RS17830 0.22 -0.8 3,706,355 + BWI76_RS17830 0.22 -2.3 3,706,377 - BWI76_RS17830 0.24 +2.3 3,706,647 - BWI76_RS17830 0.49 +0.1 3,706,665 + BWI76_RS17830 0.50 +0.0 3,706,885 + BWI76_RS17830 0.71 +0.9 3,706,913 - BWI76_RS17830 0.73 +0.7 3,706,996 - BWI76_RS17830 0.81 -3.6
Or see this region's nucleotide sequence