Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16465 and BWI76_RS16470 are separated by 142 nucleotides BWI76_RS16470 and BWI76_RS16475 are separated by 53 nucleotides BWI76_RS16475 and BWI76_RS16480 are separated by 79 nucleotides BWI76_RS16480 and BWI76_RS16485 are separated by 106 nucleotides
BWI76_RS16465: BWI76_RS16465 - DUF1289 domain-containing protein, at 3,415,061 to 3,415,300
_RS16465
BWI76_RS16470: BWI76_RS16470 - transcriptional repressor NemR, at 3,415,443 to 3,416,042
_RS16470
BWI76_RS16475: BWI76_RS16475 - alkene reductase, at 3,416,096 to 3,417,193
_RS16475
BWI76_RS16480: BWI76_RS16480 - lactoylglutathione lyase, at 3,417,273 to 3,417,680
_RS16480
BWI76_RS16485: BWI76_RS16485 - ribonuclease T, at 3,417,787 to 3,418,431
_RS16485
Position (kb) 3416
3417
3418 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 3415.448 kb on + strand at 3415.499 kb on + strand at 3415.619 kb on + strand, within BWI76_RS16470 at 3416.082 kb on + strand at 3416.187 kb on + strand at 3416.195 kb on - strand at 3416.213 kb on - strand, within BWI76_RS16475 at 3416.290 kb on + strand, within BWI76_RS16475 at 3416.325 kb on + strand, within BWI76_RS16475 at 3416.325 kb on + strand, within BWI76_RS16475 at 3416.333 kb on - strand, within BWI76_RS16475 at 3416.717 kb on + strand, within BWI76_RS16475 at 3416.725 kb on - strand, within BWI76_RS16475 at 3417.036 kb on + strand, within BWI76_RS16475 at 3417.229 kb on + strand at 3417.237 kb on - strand at 3417.334 kb on + strand, within BWI76_RS16480 at 3417.476 kb on + strand, within BWI76_RS16480 at 3417.527 kb on - strand, within BWI76_RS16480 at 3417.658 kb on + strand at 3417.804 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 3,415,448 + +0.7 3,415,499 + -0.0 3,415,619 + BWI76_RS16470 0.29 +0.6 3,416,082 + -2.6 3,416,187 + -2.7 3,416,195 - +1.2 3,416,213 - BWI76_RS16475 0.11 +2.0 3,416,290 + BWI76_RS16475 0.18 +0.4 3,416,325 + BWI76_RS16475 0.21 -0.2 3,416,325 + BWI76_RS16475 0.21 +1.7 3,416,333 - BWI76_RS16475 0.22 -0.2 3,416,717 + BWI76_RS16475 0.57 +0.0 3,416,725 - BWI76_RS16475 0.57 -1.8 3,417,036 + BWI76_RS16475 0.86 +3.9 3,417,229 + -1.3 3,417,237 - -1.0 3,417,334 + BWI76_RS16480 0.15 -0.2 3,417,476 + BWI76_RS16480 0.50 -1.0 3,417,527 - BWI76_RS16480 0.62 -0.9 3,417,658 + -1.6 3,417,804 + -2.1
Or see this region's nucleotide sequence