Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15715 and BWI76_RS15720 overlap by 8 nucleotides BWI76_RS15720 and BWI76_RS15725 overlap by 1 nucleotides BWI76_RS15725 and BWI76_RS15730 are separated by 260 nucleotides BWI76_RS15730 and BWI76_RS15735 overlap by 8 nucleotides BWI76_RS15735 and BWI76_RS15740 overlap by 4 nucleotides BWI76_RS15740 and BWI76_RS15745 overlap by 1 nucleotides
BWI76_RS15715: BWI76_RS15715 - putative ninG protein, at 3,278,720 to 3,279,358
_RS15715
BWI76_RS15720: BWI76_RS15720 - prophage protein NinE, at 3,279,351 to 3,279,521
_RS15720
BWI76_RS15725: BWI76_RS15725 - hypothetical protein, at 3,279,521 to 3,279,976
_RS15725
BWI76_RS15730: BWI76_RS15730 - hypothetical protein, at 3,280,237 to 3,280,467
_RS15730
BWI76_RS15735: BWI76_RS15735 - hypothetical protein, at 3,280,460 to 3,281,005
_RS15735
BWI76_RS15740: BWI76_RS15740 - HNH endonuclease, at 3,281,002 to 3,281,382
_RS15740
BWI76_RS15745: BWI76_RS15745 - hypothetical protein, at 3,281,382 to 3,282,116
_RS15745
Position (kb)
3280
3281 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3279.374 kb on - strand, within BWI76_RS15720 at 3279.448 kb on - strand, within BWI76_RS15720 at 3279.550 kb on - strand at 3279.838 kb on + strand, within BWI76_RS15725 at 3279.846 kb on - strand, within BWI76_RS15725 at 3279.864 kb on + strand, within BWI76_RS15725 at 3279.949 kb on + strand at 3280.350 kb on - strand, within BWI76_RS15730 at 3280.459 kb on - strand at 3280.712 kb on + strand, within BWI76_RS15735 at 3280.717 kb on - strand, within BWI76_RS15735 at 3280.976 kb on - strand at 3281.124 kb on - strand, within BWI76_RS15740 at 3281.236 kb on + strand, within BWI76_RS15740 at 3281.238 kb on + strand, within BWI76_RS15740
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 3,279,374 - BWI76_RS15720 0.13 -1.6 3,279,448 - BWI76_RS15720 0.57 +0.2 3,279,550 - +1.5 3,279,838 + BWI76_RS15725 0.70 +1.6 3,279,846 - BWI76_RS15725 0.71 +0.1 3,279,864 + BWI76_RS15725 0.75 -0.7 3,279,949 + -0.3 3,280,350 - BWI76_RS15730 0.49 -3.0 3,280,459 - +0.9 3,280,712 + BWI76_RS15735 0.46 -2.2 3,280,717 - BWI76_RS15735 0.47 -0.6 3,280,976 - -2.2 3,281,124 - BWI76_RS15740 0.32 -0.3 3,281,236 + BWI76_RS15740 0.61 +0.4 3,281,238 + BWI76_RS15740 0.62 -1.2
Or see this region's nucleotide sequence