Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15585 and BWI76_RS15590 are separated by 182 nucleotides BWI76_RS15590 and BWI76_RS15595 are separated by 59 nucleotides BWI76_RS15595 and BWI76_RS15600 are separated by 58 nucleotides BWI76_RS15600 and BWI76_RS15605 overlap by 4 nucleotides BWI76_RS15605 and BWI76_RS15610 are separated by 2 nucleotides
BWI76_RS15585: BWI76_RS15585 - DNA-binding protein, at 3,262,705 to 3,263,067
_RS15585
BWI76_RS15590: BWI76_RS15590 - hypothetical protein, at 3,263,250 to 3,263,957
_RS15590
BWI76_RS15595: BWI76_RS15595 - hypothetical protein, at 3,264,017 to 3,264,781
_RS15595
BWI76_RS15600: BWI76_RS15600 - hypothetical protein, at 3,264,840 to 3,265,223
_RS15600
BWI76_RS15605: BWI76_RS15605 - hypothetical protein, at 3,265,220 to 3,265,588
_RS15605
BWI76_RS15610: BWI76_RS15610 - hypothetical protein, at 3,265,591 to 3,265,953
_RS15610
Position (kb)
3264
3265 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3263.301 kb on + strand at 3263.403 kb on + strand, within BWI76_RS15590 at 3263.516 kb on + strand, within BWI76_RS15590 at 3263.693 kb on - strand, within BWI76_RS15590 at 3263.836 kb on - strand, within BWI76_RS15590 at 3264.104 kb on - strand, within BWI76_RS15595 at 3264.185 kb on + strand, within BWI76_RS15595 at 3264.185 kb on + strand, within BWI76_RS15595 at 3264.312 kb on + strand, within BWI76_RS15595 at 3264.444 kb on - strand, within BWI76_RS15595 at 3264.675 kb on - strand, within BWI76_RS15595 at 3264.713 kb on + strand at 3264.721 kb on - strand at 3264.721 kb on - strand at 3264.721 kb on - strand at 3264.721 kb on - strand at 3264.781 kb on - strand at 3264.869 kb on + strand at 3264.898 kb on + strand, within BWI76_RS15600 at 3265.209 kb on + strand at 3265.292 kb on - strand, within BWI76_RS15605 at 3265.517 kb on - strand, within BWI76_RS15605
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 3,263,301 + +1.7 3,263,403 + BWI76_RS15590 0.22 +1.6 3,263,516 + BWI76_RS15590 0.38 -3.9 3,263,693 - BWI76_RS15590 0.63 -0.7 3,263,836 - BWI76_RS15590 0.83 -1.7 3,264,104 - BWI76_RS15595 0.11 +0.1 3,264,185 + BWI76_RS15595 0.22 -0.7 3,264,185 + BWI76_RS15595 0.22 -4.3 3,264,312 + BWI76_RS15595 0.39 -3.1 3,264,444 - BWI76_RS15595 0.56 -1.8 3,264,675 - BWI76_RS15595 0.86 -1.1 3,264,713 + -3.0 3,264,721 - -1.1 3,264,721 - +0.5 3,264,721 - -0.5 3,264,721 - -3.0 3,264,781 - +1.0 3,264,869 + -1.7 3,264,898 + BWI76_RS15600 0.15 -0.6 3,265,209 + -2.3 3,265,292 - BWI76_RS15605 0.20 -2.2 3,265,517 - BWI76_RS15605 0.80 +0.1
Or see this region's nucleotide sequence