Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14810 and BWI76_RS14815 are separated by 21 nucleotides BWI76_RS14815 and BWI76_RS14820 are separated by 98 nucleotides BWI76_RS14820 and BWI76_RS14825 are separated by 210 nucleotides BWI76_RS14825 and BWI76_RS14830 overlap by 1 nucleotides
BWI76_RS14810: BWI76_RS14810 - carboxymuconolactone decarboxylase, at 3,104,097 to 3,104,897
_RS14810
BWI76_RS14815: BWI76_RS14815 - MdaB protein, at 3,104,919 to 3,105,515
_RS14815
BWI76_RS14820: BWI76_RS14820 - LysR family transcriptional regulator, at 3,105,614 to 3,106,522
_RS14820
BWI76_RS14825: BWI76_RS14825 - dienelactone hydrolase family protein, at 3,106,733 to 3,107,332
_RS14825
BWI76_RS14830: BWI76_RS14830 - hypothetical protein, at 3,107,332 to 3,107,904
_RS14830
Position (kb)
3105
3106
3107 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3104.648 kb on + strand, within BWI76_RS14810 at 3104.656 kb on - strand, within BWI76_RS14810 at 3104.702 kb on - strand, within BWI76_RS14810 at 3104.726 kb on - strand, within BWI76_RS14810 at 3104.736 kb on - strand, within BWI76_RS14810 at 3104.790 kb on + strand, within BWI76_RS14810 at 3104.790 kb on + strand, within BWI76_RS14810 at 3105.041 kb on + strand, within BWI76_RS14815 at 3105.059 kb on + strand, within BWI76_RS14815 at 3105.067 kb on - strand, within BWI76_RS14815 at 3105.067 kb on - strand, within BWI76_RS14815 at 3105.393 kb on - strand, within BWI76_RS14815 at 3105.432 kb on + strand, within BWI76_RS14815 at 3105.475 kb on + strand at 3105.676 kb on + strand at 3106.369 kb on + strand, within BWI76_RS14820 at 3106.660 kb on - strand at 3106.674 kb on - strand at 3106.846 kb on - strand, within BWI76_RS14825 at 3106.983 kb on + strand, within BWI76_RS14825
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 3,104,648 + BWI76_RS14810 0.69 +0.5 3,104,656 - BWI76_RS14810 0.70 -0.9 3,104,702 - BWI76_RS14810 0.76 +0.9 3,104,726 - BWI76_RS14810 0.79 -0.9 3,104,736 - BWI76_RS14810 0.80 +0.7 3,104,790 + BWI76_RS14810 0.87 -1.5 3,104,790 + BWI76_RS14810 0.87 +1.9 3,105,041 + BWI76_RS14815 0.20 -1.3 3,105,059 + BWI76_RS14815 0.23 +0.2 3,105,067 - BWI76_RS14815 0.25 +0.3 3,105,067 - BWI76_RS14815 0.25 +0.5 3,105,393 - BWI76_RS14815 0.79 -0.1 3,105,432 + BWI76_RS14815 0.86 -1.0 3,105,475 + +0.7 3,105,676 + +0.4 3,106,369 + BWI76_RS14820 0.83 -2.2 3,106,660 - -0.8 3,106,674 - -0.7 3,106,846 - BWI76_RS14825 0.19 -1.2 3,106,983 + BWI76_RS14825 0.42 +1.3
Or see this region's nucleotide sequence