Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14290

Experiment: KLEB17 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14275 and BWI76_RS14280 are separated by 74 nucleotidesBWI76_RS14280 and BWI76_RS14285 are separated by 310 nucleotidesBWI76_RS14285 and BWI76_RS14290 are separated by 42 nucleotidesBWI76_RS14290 and BWI76_RS14295 are separated by 370 nucleotides BWI76_RS14275: BWI76_RS14275 - hypothetical protein, at 2,985,593 to 2,986,789 _RS14275 BWI76_RS14280: BWI76_RS14280 - hypothetical protein, at 2,986,864 to 2,987,121 _RS14280 BWI76_RS14285: BWI76_RS14285 - IS3 family transposase, at 2,987,432 to 2,987,722 _RS14285 BWI76_RS14290: BWI76_RS14290 - AAA family ATPase, at 2,987,765 to 2,988,368 _RS14290 BWI76_RS14295: BWI76_RS14295 - IS630 family transposase, at 2,988,739 to 2,989,680 _RS14295 Position (kb) 2987 2988 2989Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2986.839 kb on - strandat 2986.875 kb on + strandat 2986.942 kb on + strand, within BWI76_RS14280at 2986.993 kb on + strand, within BWI76_RS14280at 2987.043 kb on - strand, within BWI76_RS14280at 2987.173 kb on + strandat 2987.185 kb on - strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.250 kb on - strandat 2987.275 kb on + strandat 2987.276 kb on + strandat 2987.293 kb on - strandat 2987.401 kb on + strandat 2987.446 kb on + strandat 2987.481 kb on - strand, within BWI76_RS14285at 2987.491 kb on - strand, within BWI76_RS14285at 2987.514 kb on - strand, within BWI76_RS14285at 2987.520 kb on + strand, within BWI76_RS14285at 2987.524 kb on + strand, within BWI76_RS14285at 2987.534 kb on + strand, within BWI76_RS14285at 2987.534 kb on + strand, within BWI76_RS14285at 2987.878 kb on + strand, within BWI76_RS14290at 2987.886 kb on - strand, within BWI76_RS14290at 2987.898 kb on + strand, within BWI76_RS14290at 2987.921 kb on + strand, within BWI76_RS14290at 2987.955 kb on + strand, within BWI76_RS14290at 2988.029 kb on + strand, within BWI76_RS14290at 2988.079 kb on + strand, within BWI76_RS14290at 2988.231 kb on - strand, within BWI76_RS14290at 2988.344 kb on + strandat 2988.463 kb on - strandat 2988.472 kb on - strandat 2988.516 kb on + strandat 2988.531 kb on - strandat 2988.586 kb on - strandat 2988.596 kb on - strandat 2988.621 kb on - strandat 2988.808 kb on + strandat 2988.840 kb on + strand, within BWI76_RS14295at 2988.999 kb on - strand, within BWI76_RS14295at 2989.053 kb on - strand, within BWI76_RS14295at 2989.125 kb on - strand, within BWI76_RS14295at 2989.275 kb on + strand, within BWI76_RS14295at 2989.275 kb on + strand, within BWI76_RS14295at 2989.275 kb on + strand, within BWI76_RS14295at 2989.283 kb on - strand, within BWI76_RS14295at 2989.283 kb on - strand, within BWI76_RS14295at 2989.286 kb on + strand, within BWI76_RS14295at 2989.294 kb on - strand, within BWI76_RS14295

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB17 MOI 1
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2,986,839 - +0.9
2,986,875 + -1.6
2,986,942 + BWI76_RS14280 0.30 +0.4
2,986,993 + BWI76_RS14280 0.50 -0.8
2,987,043 - BWI76_RS14280 0.69 +0.3
2,987,173 + -0.7
2,987,185 - -1.7
2,987,194 + -1.2
2,987,194 + -2.3
2,987,194 + -3.7
2,987,194 + -1.0
2,987,194 + +0.1
2,987,194 + -0.7
2,987,194 + +0.2
2,987,194 + -2.1
2,987,194 + -0.7
2,987,194 + -0.7
2,987,194 + -3.0
2,987,194 + -0.6
2,987,194 + -0.6
2,987,194 + -0.8
2,987,194 + -0.4
2,987,202 - -1.2
2,987,202 - -0.2
2,987,202 - -0.9
2,987,202 - -1.7
2,987,202 - +1.1
2,987,202 - -3.4
2,987,202 - +0.3
2,987,202 - -2.3
2,987,202 - -0.6
2,987,202 - -0.9
2,987,202 - -3.5
2,987,250 - -0.3
2,987,275 + -1.0
2,987,276 + -1.8
2,987,293 - -2.8
2,987,401 + -0.4
2,987,446 + -0.0
2,987,481 - BWI76_RS14285 0.17 +1.2
2,987,491 - BWI76_RS14285 0.20 +2.5
2,987,514 - BWI76_RS14285 0.28 +0.6
2,987,520 + BWI76_RS14285 0.30 +0.2
2,987,524 + BWI76_RS14285 0.32 -2.0
2,987,534 + BWI76_RS14285 0.35 -1.1
2,987,534 + BWI76_RS14285 0.35 -0.6
2,987,878 + BWI76_RS14290 0.19 -0.7
2,987,886 - BWI76_RS14290 0.20 -2.6
2,987,898 + BWI76_RS14290 0.22 +2.7
2,987,921 + BWI76_RS14290 0.26 -1.1
2,987,955 + BWI76_RS14290 0.31 -0.9
2,988,029 + BWI76_RS14290 0.44 +1.3
2,988,079 + BWI76_RS14290 0.52 -2.8
2,988,231 - BWI76_RS14290 0.77 -1.7
2,988,344 + -1.2
2,988,463 - -0.4
2,988,472 - -0.3
2,988,516 + +2.2
2,988,531 - +0.5
2,988,586 - +0.2
2,988,596 - -0.4
2,988,621 - +0.4
2,988,808 + +0.7
2,988,840 + BWI76_RS14295 0.11 +1.1
2,988,999 - BWI76_RS14295 0.28 +0.5
2,989,053 - BWI76_RS14295 0.33 +2.1
2,989,125 - BWI76_RS14295 0.41 +0.0
2,989,275 + BWI76_RS14295 0.57 -0.2
2,989,275 + BWI76_RS14295 0.57 +0.7
2,989,275 + BWI76_RS14295 0.57 -1.5
2,989,283 - BWI76_RS14295 0.58 +0.2
2,989,283 - BWI76_RS14295 0.58 -1.9
2,989,286 + BWI76_RS14295 0.58 -1.1
2,989,294 - BWI76_RS14295 0.59 +0.2

Or see this region's nucleotide sequence