Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13285 and BWI76_RS13290 overlap by 4 nucleotides BWI76_RS13290 and BWI76_RS13295 are separated by 239 nucleotides BWI76_RS13295 and BWI76_RS13300 are separated by 42 nucleotides
BWI76_RS13285: BWI76_RS13285 - protocatechuate 3,4-dioxygenase subunit alpha, at 2,770,001 to 2,770,621
_RS13285
BWI76_RS13290: BWI76_RS13290 - protocatechuate 3,4-dioxygenase subunit beta, at 2,770,618 to 2,771,358
_RS13290
BWI76_RS13295: BWI76_RS13295 - DUF1338 domain-containing protein, at 2,771,598 to 2,772,941
_RS13295
BWI76_RS13300: BWI76_RS13300 - carboxylesterase, at 2,772,984 to 2,774,492
_RS13300
Position (kb)
2771
2772
2773 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2770.653 kb on + strand at 2770.661 kb on - strand at 2770.888 kb on + strand, within BWI76_RS13290 at 2770.919 kb on - strand, within BWI76_RS13290 at 2771.203 kb on - strand, within BWI76_RS13290 at 2771.475 kb on + strand at 2771.602 kb on - strand at 2771.622 kb on - strand at 2771.689 kb on - strand at 2771.718 kb on - strand at 2771.737 kb on + strand, within BWI76_RS13295 at 2771.759 kb on + strand, within BWI76_RS13295 at 2772.295 kb on + strand, within BWI76_RS13295 at 2772.445 kb on + strand, within BWI76_RS13295 at 2772.476 kb on - strand, within BWI76_RS13295 at 2772.518 kb on - strand, within BWI76_RS13295 at 2772.518 kb on - strand, within BWI76_RS13295 at 2772.594 kb on + strand, within BWI76_RS13295 at 2773.015 kb on - strand at 2773.167 kb on + strand, within BWI76_RS13300 at 2773.240 kb on + strand, within BWI76_RS13300 at 2773.367 kb on - strand at 2773.727 kb on + strand, within BWI76_RS13300
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 2,770,653 + -0.4 2,770,661 - -0.4 2,770,888 + BWI76_RS13290 0.36 -1.6 2,770,919 - BWI76_RS13290 0.41 -1.8 2,771,203 - BWI76_RS13290 0.79 +0.2 2,771,475 + -0.0 2,771,602 - -0.8 2,771,622 - +1.1 2,771,689 - -1.5 2,771,718 - +0.4 2,771,737 + BWI76_RS13295 0.10 -3.4 2,771,759 + BWI76_RS13295 0.12 +0.4 2,772,295 + BWI76_RS13295 0.52 +0.2 2,772,445 + BWI76_RS13295 0.63 -0.2 2,772,476 - BWI76_RS13295 0.65 -0.1 2,772,518 - BWI76_RS13295 0.68 -0.2 2,772,518 - BWI76_RS13295 0.68 +0.1 2,772,594 + BWI76_RS13295 0.74 -0.2 2,773,015 - -1.5 2,773,167 + BWI76_RS13300 0.12 -3.6 2,773,240 + BWI76_RS13300 0.17 -0.9 2,773,367 - +0.4 2,773,727 + BWI76_RS13300 0.49 +0.4
Or see this region's nucleotide sequence