Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13260 and BWI76_RS13265 are separated by 51 nucleotides BWI76_RS13265 and BWI76_RS13270 are separated by 173 nucleotides
BWI76_RS13260: BWI76_RS13260 - S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase, at 2,764,618 to 2,765,736
_RS13260
BWI76_RS13265: BWI76_RS13265 - hypothetical protein, at 2,765,788 to 2,766,066
_RS13265
BWI76_RS13270: BWI76_RS13270 - membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family, at 2,766,240 to 2,768,612
_RS13270
Position (kb)
2765
2766
2767 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2764.792 kb on + strand, within BWI76_RS13260 at 2765.157 kb on - strand, within BWI76_RS13260 at 2765.162 kb on - strand, within BWI76_RS13260 at 2765.249 kb on - strand, within BWI76_RS13260 at 2765.256 kb on - strand, within BWI76_RS13260 at 2765.340 kb on + strand, within BWI76_RS13260 at 2765.572 kb on + strand, within BWI76_RS13260 at 2765.654 kb on - strand at 2765.840 kb on - strand, within BWI76_RS13265 at 2765.883 kb on - strand, within BWI76_RS13265 at 2765.883 kb on - strand, within BWI76_RS13265 at 2765.998 kb on + strand, within BWI76_RS13265 at 2767.015 kb on + strand, within BWI76_RS13270 at 2767.023 kb on - strand, within BWI76_RS13270
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 2,764,792 + BWI76_RS13260 0.16 -0.6 2,765,157 - BWI76_RS13260 0.48 +0.4 2,765,162 - BWI76_RS13260 0.49 -2.2 2,765,249 - BWI76_RS13260 0.56 -0.6 2,765,256 - BWI76_RS13260 0.57 -0.3 2,765,340 + BWI76_RS13260 0.65 -0.5 2,765,572 + BWI76_RS13260 0.85 -0.4 2,765,654 - +0.7 2,765,840 - BWI76_RS13265 0.19 +2.3 2,765,883 - BWI76_RS13265 0.34 -0.6 2,765,883 - BWI76_RS13265 0.34 +2.4 2,765,998 + BWI76_RS13265 0.75 +0.3 2,767,015 + BWI76_RS13270 0.33 +1.1 2,767,023 - BWI76_RS13270 0.33 -0.3
Or see this region's nucleotide sequence