Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12865 and BWI76_RS12870 are separated by 23 nucleotides BWI76_RS12870 and BWI76_RS12875 are separated by 159 nucleotides BWI76_RS12875 and BWI76_RS12880 are separated by 72 nucleotides
BWI76_RS12865: BWI76_RS12865 - acetylornithine deacetylase, at 2,680,853 to 2,682,022
_RS12865
BWI76_RS12870: BWI76_RS12870 - putative phytanoyl-CoA dioxygenase, at 2,682,046 to 2,682,861
_RS12870
BWI76_RS12875: BWI76_RS12875 - bifunctional deaminase-reductase, at 2,683,021 to 2,683,557
_RS12875
BWI76_RS12880: BWI76_RS12880 - aspartate-semialdehyde dehydrogenase, at 2,683,630 to 2,684,748
_RS12880
Position (kb)
2683
2684 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2682.117 kb on + strand at 2682.117 kb on + strand at 2682.205 kb on - strand, within BWI76_RS12870 at 2682.302 kb on + strand, within BWI76_RS12870 at 2682.418 kb on + strand, within BWI76_RS12870 at 2682.797 kb on + strand at 2682.911 kb on + strand at 2683.019 kb on + strand at 2683.148 kb on - strand, within BWI76_RS12875 at 2683.188 kb on + strand, within BWI76_RS12875 at 2683.225 kb on - strand, within BWI76_RS12875 at 2683.379 kb on - strand, within BWI76_RS12875 at 2683.526 kb on - strand at 2683.568 kb on + strand at 2683.573 kb on - strand at 2683.576 kb on - strand at 2683.612 kb on - strand at 2683.663 kb on + strand at 2683.700 kb on + strand at 2683.713 kb on - strand at 2683.725 kb on + strand at 2683.791 kb on - strand, within BWI76_RS12880 at 2683.801 kb on - strand, within BWI76_RS12880 at 2683.811 kb on - strand, within BWI76_RS12880 at 2683.895 kb on + strand, within BWI76_RS12880 at 2683.909 kb on + strand, within BWI76_RS12880 at 2683.909 kb on + strand, within BWI76_RS12880 at 2683.909 kb on + strand, within BWI76_RS12880 at 2683.909 kb on + strand, within BWI76_RS12880 at 2683.909 kb on + strand, within BWI76_RS12880 at 2683.917 kb on - strand, within BWI76_RS12880 at 2684.032 kb on + strand, within BWI76_RS12880 at 2684.088 kb on - strand, within BWI76_RS12880 at 2684.176 kb on - strand, within BWI76_RS12880 at 2684.239 kb on + strand, within BWI76_RS12880 at 2684.241 kb on - strand, within BWI76_RS12880 at 2684.249 kb on - strand, within BWI76_RS12880 at 2684.289 kb on - strand, within BWI76_RS12880 at 2684.289 kb on - strand, within BWI76_RS12880 at 2684.289 kb on - strand, within BWI76_RS12880 at 2684.303 kb on - strand, within BWI76_RS12880 at 2684.303 kb on - strand, within BWI76_RS12880 at 2684.308 kb on - strand, within BWI76_RS12880 at 2684.308 kb on - strand at 2684.337 kb on + strand, within BWI76_RS12880 at 2684.342 kb on - strand, within BWI76_RS12880 at 2684.347 kb on - strand, within BWI76_RS12880 at 2684.439 kb on - strand, within BWI76_RS12880 at 2684.456 kb on - strand, within BWI76_RS12880 at 2684.468 kb on - strand, within BWI76_RS12880 at 2684.468 kb on - strand, within BWI76_RS12880 at 2684.496 kb on + strand, within BWI76_RS12880 at 2684.511 kb on - strand, within BWI76_RS12880 at 2684.522 kb on + strand, within BWI76_RS12880 at 2684.544 kb on - strand, within BWI76_RS12880
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 2,682,117 + +0.2 2,682,117 + -0.6 2,682,205 - BWI76_RS12870 0.19 -0.8 2,682,302 + BWI76_RS12870 0.31 -0.3 2,682,418 + BWI76_RS12870 0.46 +1.2 2,682,797 + -0.3 2,682,911 + -1.4 2,683,019 + +0.0 2,683,148 - BWI76_RS12875 0.24 -0.6 2,683,188 + BWI76_RS12875 0.31 -1.5 2,683,225 - BWI76_RS12875 0.38 -2.8 2,683,379 - BWI76_RS12875 0.67 -1.4 2,683,526 - -1.1 2,683,568 + -0.5 2,683,573 - +1.1 2,683,576 - +0.8 2,683,612 - +0.9 2,683,663 + -0.5 2,683,700 + +0.1 2,683,713 - +0.6 2,683,725 + +1.2 2,683,791 - BWI76_RS12880 0.14 -2.3 2,683,801 - BWI76_RS12880 0.15 +0.3 2,683,811 - BWI76_RS12880 0.16 +0.5 2,683,895 + BWI76_RS12880 0.24 -3.5 2,683,909 + BWI76_RS12880 0.25 -0.8 2,683,909 + BWI76_RS12880 0.25 -1.0 2,683,909 + BWI76_RS12880 0.25 +1.8 2,683,909 + BWI76_RS12880 0.25 -1.1 2,683,909 + BWI76_RS12880 0.25 +0.5 2,683,917 - BWI76_RS12880 0.26 -2.2 2,684,032 + BWI76_RS12880 0.36 -3.1 2,684,088 - BWI76_RS12880 0.41 +0.3 2,684,176 - BWI76_RS12880 0.49 -1.8 2,684,239 + BWI76_RS12880 0.54 +0.6 2,684,241 - BWI76_RS12880 0.55 +0.8 2,684,249 - BWI76_RS12880 0.55 +0.0 2,684,289 - BWI76_RS12880 0.59 -1.5 2,684,289 - BWI76_RS12880 0.59 +0.6 2,684,289 - BWI76_RS12880 0.59 -1.0 2,684,303 - BWI76_RS12880 0.60 +2.6 2,684,303 - BWI76_RS12880 0.60 -2.2 2,684,308 - BWI76_RS12880 0.61 -3.0 2,684,308 - -0.8 2,684,337 + BWI76_RS12880 0.63 -2.5 2,684,342 - BWI76_RS12880 0.64 +1.4 2,684,347 - BWI76_RS12880 0.64 +1.3 2,684,439 - BWI76_RS12880 0.72 +1.4 2,684,456 - BWI76_RS12880 0.74 -1.0 2,684,468 - BWI76_RS12880 0.75 -0.5 2,684,468 - BWI76_RS12880 0.75 -1.0 2,684,496 + BWI76_RS12880 0.77 -0.3 2,684,511 - BWI76_RS12880 0.79 -1.4 2,684,522 + BWI76_RS12880 0.80 +0.0 2,684,544 - BWI76_RS12880 0.82 -2.4
Or see this region's nucleotide sequence