Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12850 and BWI76_RS12855 are separated by 23 nucleotides BWI76_RS12855 and BWI76_RS12860 overlap by 4 nucleotides BWI76_RS12860 and BWI76_RS12865 overlap by 8 nucleotides
BWI76_RS12850: BWI76_RS12850 - hypothetical protein, at 2,678,472 to 2,679,731
_RS12850
BWI76_RS12855: BWI76_RS12855 - enamine deaminase RidA, at 2,679,755 to 2,680,189
_RS12855
BWI76_RS12860: BWI76_RS12860 - hypothetical protein, at 2,680,186 to 2,680,860
_RS12860
BWI76_RS12865: BWI76_RS12865 - acetylornithine deacetylase, at 2,680,853 to 2,682,022
_RS12865
Position (kb)
2680
2681 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2679.228 kb on - strand, within BWI76_RS12850 at 2679.241 kb on + strand, within BWI76_RS12850 at 2679.249 kb on - strand, within BWI76_RS12850 at 2679.347 kb on + strand, within BWI76_RS12850 at 2679.389 kb on - strand, within BWI76_RS12850 at 2679.769 kb on - strand at 2679.837 kb on - strand, within BWI76_RS12855 at 2680.044 kb on + strand, within BWI76_RS12855 at 2680.303 kb on + strand, within BWI76_RS12860 at 2680.518 kb on - strand, within BWI76_RS12860 at 2681.019 kb on - strand, within BWI76_RS12865 at 2681.110 kb on - strand, within BWI76_RS12865 at 2681.268 kb on + strand, within BWI76_RS12865 at 2681.368 kb on - strand, within BWI76_RS12865 at 2681.368 kb on - strand, within BWI76_RS12865 at 2681.518 kb on - strand, within BWI76_RS12865
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 2,679,228 - BWI76_RS12850 0.60 +0.5 2,679,241 + BWI76_RS12850 0.61 +0.8 2,679,249 - BWI76_RS12850 0.62 +0.3 2,679,347 + BWI76_RS12850 0.69 +0.7 2,679,389 - BWI76_RS12850 0.73 +1.1 2,679,769 - -0.2 2,679,837 - BWI76_RS12855 0.19 -2.3 2,680,044 + BWI76_RS12855 0.66 -3.2 2,680,303 + BWI76_RS12860 0.17 +2.0 2,680,518 - BWI76_RS12860 0.49 +0.4 2,681,019 - BWI76_RS12865 0.14 -1.9 2,681,110 - BWI76_RS12865 0.22 +0.4 2,681,268 + BWI76_RS12865 0.35 +1.6 2,681,368 - BWI76_RS12865 0.44 -1.8 2,681,368 - BWI76_RS12865 0.44 -2.0 2,681,518 - BWI76_RS12865 0.57 -0.3
Or see this region's nucleotide sequence