Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11460 and BWI76_RS11465 are separated by 152 nucleotides BWI76_RS11465 and BWI76_RS11470 are separated by 27 nucleotides BWI76_RS11470 and BWI76_RS11475 are separated by 361 nucleotides
BWI76_RS11460: BWI76_RS11460 - DUF441 family protein, at 2,411,742 to 2,412,188
_RS11460
BWI76_RS11465: BWI76_RS11465 - hypothetical protein, at 2,412,341 to 2,412,841
_RS11465
BWI76_RS11470: BWI76_RS11470 - sensor domain-containing diguanylate cyclase, at 2,412,869 to 2,414,371
_RS11470
BWI76_RS11475: BWI76_RS11475 - glycosyl transferase, at 2,414,733 to 2,415,890
_RS11475
Position (kb)
2412
2413
2414
2415 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2412.074 kb on + strand, within BWI76_RS11460 at 2412.164 kb on - strand at 2412.181 kb on - strand at 2412.197 kb on - strand at 2412.197 kb on - strand at 2412.367 kb on + strand at 2412.558 kb on + strand, within BWI76_RS11465 at 2412.680 kb on + strand, within BWI76_RS11465 at 2412.696 kb on + strand, within BWI76_RS11465 at 2412.773 kb on - strand, within BWI76_RS11465 at 2412.980 kb on + strand at 2413.026 kb on + strand, within BWI76_RS11470 at 2413.041 kb on - strand, within BWI76_RS11470 at 2413.125 kb on - strand at 2413.438 kb on + strand, within BWI76_RS11470 at 2413.438 kb on + strand, within BWI76_RS11470 at 2413.470 kb on + strand, within BWI76_RS11470 at 2413.799 kb on + strand, within BWI76_RS11470 at 2413.799 kb on + strand, within BWI76_RS11470 at 2413.799 kb on + strand at 2413.807 kb on - strand, within BWI76_RS11470 at 2413.814 kb on - strand, within BWI76_RS11470 at 2414.021 kb on - strand, within BWI76_RS11470 at 2414.039 kb on - strand, within BWI76_RS11470 at 2414.046 kb on + strand, within BWI76_RS11470 at 2414.046 kb on + strand, within BWI76_RS11470 at 2414.049 kb on - strand, within BWI76_RS11470 at 2414.132 kb on + strand, within BWI76_RS11470 at 2414.447 kb on - strand at 2414.449 kb on + strand at 2414.454 kb on + strand at 2414.682 kb on + strand at 2414.690 kb on - strand at 2414.690 kb on - strand at 2414.690 kb on - strand at 2414.690 kb on - strand at 2414.690 kb on - strand at 2414.692 kb on + strand at 2414.692 kb on + strand at 2414.700 kb on - strand at 2414.700 kb on - strand at 2414.700 kb on - strand at 2414.700 kb on - strand at 2414.700 kb on - strand at 2414.704 kb on + strand at 2414.706 kb on + strand at 2414.706 kb on + strand at 2414.707 kb on + strand at 2414.719 kb on + strand at 2414.719 kb on + strand at 2414.727 kb on - strand at 2414.727 kb on - strand at 2414.727 kb on - strand at 2414.751 kb on + strand at 2414.751 kb on + strand at 2414.751 kb on + strand at 2414.751 kb on + strand at 2414.753 kb on + strand at 2414.759 kb on - strand at 2414.761 kb on - strand at 2414.763 kb on + strand at 2414.840 kb on + strand at 2414.840 kb on + strand at 2414.889 kb on + strand, within BWI76_RS11475 at 2414.889 kb on + strand, within BWI76_RS11475 at 2414.889 kb on + strand, within BWI76_RS11475 at 2414.889 kb on + strand, within BWI76_RS11475 at 2414.897 kb on - strand, within BWI76_RS11475 at 2414.897 kb on - strand, within BWI76_RS11475 at 2414.897 kb on - strand, within BWI76_RS11475 at 2414.897 kb on - strand, within BWI76_RS11475 at 2414.897 kb on - strand at 2414.897 kb on - strand, within BWI76_RS11475 at 2414.934 kb on + strand, within BWI76_RS11475 at 2414.964 kb on - strand, within BWI76_RS11475 at 2414.966 kb on + strand, within BWI76_RS11475 at 2414.966 kb on + strand, within BWI76_RS11475 at 2414.966 kb on + strand, within BWI76_RS11475 at 2414.966 kb on + strand, within BWI76_RS11475 at 2414.966 kb on + strand, within BWI76_RS11475 at 2414.966 kb on + strand, within BWI76_RS11475 at 2414.974 kb on - strand, within BWI76_RS11475 at 2414.978 kb on + strand, within BWI76_RS11475 at 2414.988 kb on + strand, within BWI76_RS11475 at 2414.991 kb on - strand, within BWI76_RS11475 at 2415.015 kb on + strand, within BWI76_RS11475 at 2415.025 kb on + strand, within BWI76_RS11475 at 2415.080 kb on - strand, within BWI76_RS11475
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 2,412,074 + BWI76_RS11460 0.74 +0.4 2,412,164 - +0.2 2,412,181 - -3.1 2,412,197 - +0.5 2,412,197 - +1.6 2,412,367 + -2.7 2,412,558 + BWI76_RS11465 0.43 -2.1 2,412,680 + BWI76_RS11465 0.68 -3.3 2,412,696 + BWI76_RS11465 0.71 -1.6 2,412,773 - BWI76_RS11465 0.86 -0.4 2,412,980 + -1.6 2,413,026 + BWI76_RS11470 0.10 -1.9 2,413,041 - BWI76_RS11470 0.11 +0.8 2,413,125 - +2.1 2,413,438 + BWI76_RS11470 0.38 -0.2 2,413,438 + BWI76_RS11470 0.38 -2.7 2,413,470 + BWI76_RS11470 0.40 -1.9 2,413,799 + BWI76_RS11470 0.62 -3.5 2,413,799 + BWI76_RS11470 0.62 -1.9 2,413,799 + +3.7 2,413,807 - BWI76_RS11470 0.62 -0.5 2,413,814 - BWI76_RS11470 0.63 +0.3 2,414,021 - BWI76_RS11470 0.77 -1.4 2,414,039 - BWI76_RS11470 0.78 -2.1 2,414,046 + BWI76_RS11470 0.78 -3.2 2,414,046 + BWI76_RS11470 0.78 -2.4 2,414,049 - BWI76_RS11470 0.79 -0.3 2,414,132 + BWI76_RS11470 0.84 -1.5 2,414,447 - -1.5 2,414,449 + -1.2 2,414,454 + -0.0 2,414,682 + +2.5 2,414,690 - -0.2 2,414,690 - -0.5 2,414,690 - -0.7 2,414,690 - -0.9 2,414,690 - +0.1 2,414,692 + -1.4 2,414,692 + -3.6 2,414,700 - +0.8 2,414,700 - +0.0 2,414,700 - -1.5 2,414,700 - +0.9 2,414,700 - -0.5 2,414,704 + -0.0 2,414,706 + +1.4 2,414,706 + +3.1 2,414,707 + +2.3 2,414,719 + +2.3 2,414,719 + +3.0 2,414,727 - +2.1 2,414,727 - +0.2 2,414,727 - -1.4 2,414,751 + +0.7 2,414,751 + +0.2 2,414,751 + +1.1 2,414,751 + -0.2 2,414,753 + +1.0 2,414,759 - +1.8 2,414,761 - -2.2 2,414,763 + +0.0 2,414,840 + +1.3 2,414,840 + +2.1 2,414,889 + BWI76_RS11475 0.13 +0.6 2,414,889 + BWI76_RS11475 0.13 -2.0 2,414,889 + BWI76_RS11475 0.13 +0.5 2,414,889 + BWI76_RS11475 0.13 -1.5 2,414,897 - BWI76_RS11475 0.14 -0.5 2,414,897 - BWI76_RS11475 0.14 -1.7 2,414,897 - BWI76_RS11475 0.14 -0.7 2,414,897 - BWI76_RS11475 0.14 -0.1 2,414,897 - +1.1 2,414,897 - BWI76_RS11475 0.14 +1.1 2,414,934 + BWI76_RS11475 0.17 +1.6 2,414,964 - BWI76_RS11475 0.20 +1.2 2,414,966 + BWI76_RS11475 0.20 -1.8 2,414,966 + BWI76_RS11475 0.20 -1.9 2,414,966 + BWI76_RS11475 0.20 +1.2 2,414,966 + BWI76_RS11475 0.20 +0.8 2,414,966 + BWI76_RS11475 0.20 -0.7 2,414,966 + BWI76_RS11475 0.20 -1.2 2,414,974 - BWI76_RS11475 0.21 +0.1 2,414,978 + BWI76_RS11475 0.21 -1.4 2,414,988 + BWI76_RS11475 0.22 -2.7 2,414,991 - BWI76_RS11475 0.22 -0.7 2,415,015 + BWI76_RS11475 0.24 +1.1 2,415,025 + BWI76_RS11475 0.25 -0.0 2,415,080 - BWI76_RS11475 0.30 -0.5
Or see this region's nucleotide sequence