Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10965 and BWI76_RS10970 overlap by 15 nucleotides BWI76_RS10970 and BWI76_RS10975 are separated by 73 nucleotides BWI76_RS10975 and BWI76_RS10980 are separated by 124 nucleotides BWI76_RS10980 and BWI76_RS10985 are separated by 285 nucleotides BWI76_RS10985 and BWI76_RS10990 are separated by 72 nucleotides
BWI76_RS10965: BWI76_RS10965 - sulfurtransferase, at 2,314,992 to 2,316,056
_RS10965
BWI76_RS10970: BWI76_RS10970 - DUF2770 domain-containing protein, at 2,316,042 to 2,316,155
_RS10970
BWI76_RS10975: BWI76_RS10975 - N-methyltryptophan oxidase, at 2,316,229 to 2,317,347
_RS10975
BWI76_RS10980: BWI76_RS10980 - biofilm formation regulatory protein BssS, at 2,317,472 to 2,317,726
_RS10980
BWI76_RS10985: BWI76_RS10985 - DNA damage-inducible protein I, at 2,318,012 to 2,318,257
_RS10985
BWI76_RS10990: BWI76_RS10990 - dihydroorotase, at 2,318,330 to 2,319,376
_RS10990
Position (kb)
2316
2317
2318 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2315.306 kb on + strand, within BWI76_RS10965 at 2315.320 kb on + strand, within BWI76_RS10965 at 2315.909 kb on - strand, within BWI76_RS10965 at 2316.539 kb on - strand, within BWI76_RS10975 at 2316.610 kb on - strand, within BWI76_RS10975 at 2317.096 kb on + strand, within BWI76_RS10975 at 2317.133 kb on + strand, within BWI76_RS10975 at 2317.198 kb on + strand, within BWI76_RS10975 at 2317.267 kb on + strand at 2317.274 kb on - strand at 2317.284 kb on + strand at 2317.284 kb on + strand at 2317.294 kb on + strand at 2317.337 kb on - strand at 2317.344 kb on + strand at 2317.352 kb on + strand at 2317.361 kb on - strand at 2317.367 kb on - strand at 2317.433 kb on - strand at 2317.492 kb on - strand at 2317.497 kb on - strand at 2317.517 kb on - strand, within BWI76_RS10980 at 2317.518 kb on - strand, within BWI76_RS10980 at 2317.638 kb on + strand, within BWI76_RS10980 at 2317.643 kb on + strand, within BWI76_RS10980 at 2317.670 kb on + strand, within BWI76_RS10980 at 2317.676 kb on - strand, within BWI76_RS10980 at 2317.680 kb on + strand, within BWI76_RS10980 at 2317.690 kb on + strand, within BWI76_RS10980 at 2317.697 kb on + strand, within BWI76_RS10980 at 2317.697 kb on + strand, within BWI76_RS10980 at 2317.698 kb on - strand, within BWI76_RS10980 at 2317.705 kb on - strand at 2317.705 kb on - strand at 2317.726 kb on + strand at 2317.927 kb on + strand at 2317.935 kb on - strand at 2317.945 kb on - strand at 2318.025 kb on + strand at 2318.033 kb on - strand at 2318.033 kb on - strand at 2318.035 kb on - strand at 2318.045 kb on - strand, within BWI76_RS10985 at 2318.134 kb on + strand, within BWI76_RS10985 at 2318.152 kb on - strand, within BWI76_RS10985 at 2318.193 kb on - strand, within BWI76_RS10985 at 2318.193 kb on - strand, within BWI76_RS10985
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 2,315,306 + BWI76_RS10965 0.29 -0.4 2,315,320 + BWI76_RS10965 0.31 +0.4 2,315,909 - BWI76_RS10965 0.86 -0.3 2,316,539 - BWI76_RS10975 0.28 -3.8 2,316,610 - BWI76_RS10975 0.34 -0.3 2,317,096 + BWI76_RS10975 0.77 -2.4 2,317,133 + BWI76_RS10975 0.81 -1.9 2,317,198 + BWI76_RS10975 0.87 -0.4 2,317,267 + +0.7 2,317,274 - +1.3 2,317,284 + -0.8 2,317,284 + +1.5 2,317,294 + -0.4 2,317,337 - -2.4 2,317,344 + +0.6 2,317,352 + +0.4 2,317,361 - +2.6 2,317,367 - -0.2 2,317,433 - -0.2 2,317,492 - -1.4 2,317,497 - -0.6 2,317,517 - BWI76_RS10980 0.18 -3.5 2,317,518 - BWI76_RS10980 0.18 -0.0 2,317,638 + BWI76_RS10980 0.65 +0.0 2,317,643 + BWI76_RS10980 0.67 +0.4 2,317,670 + BWI76_RS10980 0.78 -2.7 2,317,676 - BWI76_RS10980 0.80 +1.7 2,317,680 + BWI76_RS10980 0.82 +0.5 2,317,690 + BWI76_RS10980 0.85 +0.5 2,317,697 + BWI76_RS10980 0.88 -3.0 2,317,697 + BWI76_RS10980 0.88 -2.0 2,317,698 - BWI76_RS10980 0.89 -0.3 2,317,705 - -1.3 2,317,705 - -0.7 2,317,726 + -0.8 2,317,927 + -1.1 2,317,935 - +0.4 2,317,945 - +0.9 2,318,025 + +1.1 2,318,033 - +1.9 2,318,033 - +1.1 2,318,035 - +1.2 2,318,045 - BWI76_RS10985 0.13 -0.2 2,318,134 + BWI76_RS10985 0.50 -0.4 2,318,152 - BWI76_RS10985 0.57 +0.3 2,318,193 - BWI76_RS10985 0.74 +0.7 2,318,193 - BWI76_RS10985 0.74 -0.8
Or see this region's nucleotide sequence