Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10605 and BWI76_RS10610 are separated by 693 nucleotides BWI76_RS10610 and BWI76_RS10615 are separated by 182 nucleotides BWI76_RS10615 and BWI76_RS10620 are separated by 130 nucleotides
BWI76_RS10605: BWI76_RS10605 - oligogalacturonate porin, at 2,240,055 to 2,240,756
_RS10605
BWI76_RS10610: BWI76_RS10610 - helix-turn-helix transcriptional regulator, at 2,241,450 to 2,241,998
_RS10610
BWI76_RS10615: BWI76_RS10615 - chorismate mutase, at 2,242,181 to 2,242,477
_RS10615
BWI76_RS10620: BWI76_RS10620 - hypothetical protein, at 2,242,608 to 2,243,672
_RS10620
Position (kb)
2241
2242 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2240.462 kb on + strand, within BWI76_RS10605 at 2240.462 kb on + strand, within BWI76_RS10605 at 2240.467 kb on - strand, within BWI76_RS10605 at 2240.470 kb on - strand, within BWI76_RS10605 at 2240.471 kb on + strand, within BWI76_RS10605 at 2240.471 kb on + strand, within BWI76_RS10605 at 2240.471 kb on + strand, within BWI76_RS10605 at 2240.479 kb on - strand, within BWI76_RS10605 at 2240.525 kb on + strand, within BWI76_RS10605 at 2240.525 kb on + strand, within BWI76_RS10605 at 2240.525 kb on + strand, within BWI76_RS10605 at 2240.525 kb on + strand, within BWI76_RS10605 at 2240.525 kb on + strand, within BWI76_RS10605 at 2240.525 kb on + strand, within BWI76_RS10605 at 2240.533 kb on - strand, within BWI76_RS10605 at 2240.578 kb on + strand, within BWI76_RS10605 at 2240.603 kb on + strand at 2240.603 kb on + strand, within BWI76_RS10605 at 2240.611 kb on + strand, within BWI76_RS10605 at 2240.637 kb on - strand, within BWI76_RS10605 at 2240.639 kb on - strand, within BWI76_RS10605 at 2240.643 kb on + strand, within BWI76_RS10605 at 2240.687 kb on + strand at 2240.687 kb on + strand at 2240.687 kb on + strand at 2240.687 kb on + strand at 2240.687 kb on + strand at 2240.687 kb on + strand at 2240.687 kb on + strand at 2240.695 kb on + strand at 2240.716 kb on - strand at 2240.813 kb on - strand at 2240.813 kb on - strand at 2240.817 kb on - strand at 2240.846 kb on + strand at 2240.846 kb on + strand at 2240.856 kb on + strand at 2240.856 kb on + strand at 2240.857 kb on - strand at 2240.872 kb on - strand at 2240.895 kb on - strand at 2240.918 kb on + strand at 2240.924 kb on + strand at 2240.938 kb on - strand at 2240.938 kb on - strand at 2240.951 kb on + strand at 2240.953 kb on + strand at 2240.953 kb on + strand at 2240.953 kb on + strand at 2240.953 kb on + strand at 2240.953 kb on + strand at 2240.953 kb on + strand at 2240.953 kb on + strand at 2240.961 kb on - strand at 2240.961 kb on - strand at 2240.961 kb on - strand at 2240.981 kb on - strand at 2240.997 kb on + strand at 2241.296 kb on + strand at 2241.314 kb on + strand at 2241.323 kb on + strand at 2241.331 kb on - strand at 2241.331 kb on - strand at 2241.353 kb on + strand at 2241.373 kb on - strand at 2241.379 kb on + strand at 2241.402 kb on + strand at 2241.435 kb on + strand at 2241.435 kb on + strand at 2241.525 kb on + strand at 2241.525 kb on + strand, within BWI76_RS10610 at 2241.525 kb on + strand, within BWI76_RS10610 at 2241.531 kb on + strand, within BWI76_RS10610 at 2241.531 kb on + strand, within BWI76_RS10610 at 2241.531 kb on + strand, within BWI76_RS10610 at 2241.578 kb on + strand, within BWI76_RS10610 at 2241.586 kb on - strand, within BWI76_RS10610 at 2241.611 kb on + strand, within BWI76_RS10610 at 2241.616 kb on + strand, within BWI76_RS10610 at 2241.725 kb on + strand, within BWI76_RS10610 at 2241.892 kb on + strand, within BWI76_RS10610 at 2242.116 kb on + strand at 2242.214 kb on - strand, within BWI76_RS10615 at 2242.302 kb on + strand, within BWI76_RS10615 at 2242.308 kb on - strand, within BWI76_RS10615 at 2242.408 kb on + strand, within BWI76_RS10615 at 2242.408 kb on - strand, within BWI76_RS10615 at 2242.422 kb on + strand at 2242.422 kb on + strand, within BWI76_RS10615 at 2242.430 kb on - strand, within BWI76_RS10615 at 2242.452 kb on + strand at 2242.545 kb on + strand at 2242.567 kb on - strand at 2242.662 kb on + strand at 2242.707 kb on + strand at 2242.760 kb on + strand, within BWI76_RS10620 at 2242.796 kb on + strand, within BWI76_RS10620 at 2242.888 kb on + strand, within BWI76_RS10620 at 2242.922 kb on - strand, within BWI76_RS10620 at 2242.938 kb on + strand, within BWI76_RS10620 at 2242.943 kb on + strand, within BWI76_RS10620 at 2242.949 kb on - strand, within BWI76_RS10620 at 2242.951 kb on - strand, within BWI76_RS10620
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 2,240,462 + BWI76_RS10605 0.58 -2.3 2,240,462 + BWI76_RS10605 0.58 -1.6 2,240,467 - BWI76_RS10605 0.59 -0.3 2,240,470 - BWI76_RS10605 0.59 -0.6 2,240,471 + BWI76_RS10605 0.59 -0.8 2,240,471 + BWI76_RS10605 0.59 +0.2 2,240,471 + BWI76_RS10605 0.59 +1.2 2,240,479 - BWI76_RS10605 0.60 -0.8 2,240,525 + BWI76_RS10605 0.67 +0.5 2,240,525 + BWI76_RS10605 0.67 -0.8 2,240,525 + BWI76_RS10605 0.67 +2.0 2,240,525 + BWI76_RS10605 0.67 +1.0 2,240,525 + BWI76_RS10605 0.67 +0.0 2,240,525 + BWI76_RS10605 0.67 +0.2 2,240,533 - BWI76_RS10605 0.68 +2.0 2,240,578 + BWI76_RS10605 0.75 +1.0 2,240,603 + +2.0 2,240,603 + BWI76_RS10605 0.78 +3.6 2,240,611 + BWI76_RS10605 0.79 +2.0 2,240,637 - BWI76_RS10605 0.83 +2.0 2,240,639 - BWI76_RS10605 0.83 -0.8 2,240,643 + BWI76_RS10605 0.84 -1.4 2,240,687 + -0.7 2,240,687 + -0.1 2,240,687 + -2.2 2,240,687 + +0.4 2,240,687 + -2.4 2,240,687 + +2.0 2,240,687 + -2.2 2,240,695 + -1.2 2,240,716 - +0.4 2,240,813 - -0.1 2,240,813 - +0.1 2,240,817 - -0.6 2,240,846 + +0.4 2,240,846 + +0.4 2,240,856 + -0.3 2,240,856 + +1.0 2,240,857 - -0.7 2,240,872 - +2.9 2,240,895 - -0.8 2,240,918 + -2.7 2,240,924 + -0.8 2,240,938 - -1.2 2,240,938 - +3.9 2,240,951 + -0.3 2,240,953 + +0.0 2,240,953 + +1.0 2,240,953 + -2.0 2,240,953 + -0.2 2,240,953 + -0.6 2,240,953 + +0.4 2,240,953 + -0.6 2,240,961 - -0.3 2,240,961 - +0.0 2,240,961 - -0.0 2,240,981 - +1.1 2,240,997 + +1.1 2,241,296 + -3.0 2,241,314 + +0.5 2,241,323 + -0.9 2,241,331 - -1.9 2,241,331 - +0.0 2,241,353 + +0.6 2,241,373 - +0.4 2,241,379 + -2.7 2,241,402 + -0.6 2,241,435 + -0.8 2,241,435 + +0.1 2,241,525 + +2.0 2,241,525 + BWI76_RS10610 0.14 -0.9 2,241,525 + BWI76_RS10610 0.14 -1.2 2,241,531 + BWI76_RS10610 0.15 -1.3 2,241,531 + BWI76_RS10610 0.15 -1.7 2,241,531 + BWI76_RS10610 0.15 -1.2 2,241,578 + BWI76_RS10610 0.23 +0.1 2,241,586 - BWI76_RS10610 0.25 +2.0 2,241,611 + BWI76_RS10610 0.29 -0.8 2,241,616 + BWI76_RS10610 0.30 +0.2 2,241,725 + BWI76_RS10610 0.50 -0.6 2,241,892 + BWI76_RS10610 0.81 +2.0 2,242,116 + +0.4 2,242,214 - BWI76_RS10615 0.11 +1.0 2,242,302 + BWI76_RS10615 0.41 +2.3 2,242,308 - BWI76_RS10615 0.43 +1.1 2,242,408 + BWI76_RS10615 0.76 -1.0 2,242,408 - BWI76_RS10615 0.76 -0.8 2,242,422 + +0.4 2,242,422 + BWI76_RS10615 0.81 +0.3 2,242,430 - BWI76_RS10615 0.84 -0.1 2,242,452 + -1.8 2,242,545 + -1.0 2,242,567 - -0.5 2,242,662 + +1.0 2,242,707 + +4.8 2,242,760 + BWI76_RS10620 0.14 +0.9 2,242,796 + BWI76_RS10620 0.18 +1.7 2,242,888 + BWI76_RS10620 0.26 +0.1 2,242,922 - BWI76_RS10620 0.29 -0.6 2,242,938 + BWI76_RS10620 0.31 +0.7 2,242,943 + BWI76_RS10620 0.31 +0.0 2,242,949 - BWI76_RS10620 0.32 -0.1 2,242,951 - BWI76_RS10620 0.32 +0.6
Or see this region's nucleotide sequence