Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07265 and BWI76_RS07270 overlap by 8 nucleotides BWI76_RS07270 and BWI76_RS07275 overlap by 4 nucleotides BWI76_RS07275 and BWI76_RS07280 are separated by 10 nucleotides
BWI76_RS07265: BWI76_RS07265 - ABC transporter ATP-binding protein, at 1,528,943 to 1,529,662
_RS07265
BWI76_RS07270: BWI76_RS07270 - ABC transporter ATP-binding protein, at 1,529,655 to 1,530,422
_RS07270
BWI76_RS07275: BWI76_RS07275 - ABC transporter, at 1,530,419 to 1,531,525
_RS07275
BWI76_RS07280: BWI76_RS07280 - branched-chain amino acid ABC transporter permease, at 1,531,536 to 1,532,486
_RS07280
Position (kb)
1530
1531
1532 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1529.433 kb on - strand, within BWI76_RS07265 at 1529.539 kb on - strand, within BWI76_RS07265 at 1529.596 kb on - strand at 1529.596 kb on - strand at 1529.890 kb on + strand, within BWI76_RS07270 at 1529.923 kb on - strand, within BWI76_RS07270 at 1530.281 kb on + strand, within BWI76_RS07270 at 1530.289 kb on - strand, within BWI76_RS07270 at 1530.341 kb on + strand, within BWI76_RS07270 at 1530.349 kb on - strand at 1530.447 kb on + strand at 1530.487 kb on + strand at 1530.599 kb on + strand, within BWI76_RS07275 at 1530.693 kb on - strand, within BWI76_RS07275 at 1530.693 kb on - strand, within BWI76_RS07275 at 1530.751 kb on - strand, within BWI76_RS07275 at 1530.770 kb on + strand, within BWI76_RS07275 at 1530.826 kb on - strand, within BWI76_RS07275 at 1531.116 kb on - strand, within BWI76_RS07275 at 1531.150 kb on - strand at 1531.195 kb on + strand, within BWI76_RS07275 at 1531.342 kb on + strand, within BWI76_RS07275 at 1531.371 kb on + strand, within BWI76_RS07275 at 1531.371 kb on + strand, within BWI76_RS07275 at 1531.371 kb on + strand, within BWI76_RS07275 at 1531.379 kb on - strand, within BWI76_RS07275 at 1531.620 kb on + strand at 1531.658 kb on - strand, within BWI76_RS07280 at 1531.974 kb on - strand, within BWI76_RS07280 at 1532.024 kb on + strand, within BWI76_RS07280 at 1532.068 kb on - strand, within BWI76_RS07280 at 1532.272 kb on + strand, within BWI76_RS07280 at 1532.340 kb on + strand, within BWI76_RS07280 at 1532.395 kb on + strand at 1532.405 kb on - strand at 1532.405 kb on - strand at 1532.410 kb on + strand at 1532.416 kb on - strand at 1532.418 kb on - strand at 1532.446 kb on + strand at 1532.477 kb on - strand at 1532.501 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 1,529,433 - BWI76_RS07265 0.68 -2.9 1,529,539 - BWI76_RS07265 0.83 +1.0 1,529,596 - +1.0 1,529,596 - +0.5 1,529,890 + BWI76_RS07270 0.31 +1.6 1,529,923 - BWI76_RS07270 0.35 +0.8 1,530,281 + BWI76_RS07270 0.82 +1.1 1,530,289 - BWI76_RS07270 0.83 +0.1 1,530,341 + BWI76_RS07270 0.89 -0.9 1,530,349 - +2.5 1,530,447 + -0.4 1,530,487 + -0.2 1,530,599 + BWI76_RS07275 0.16 +0.6 1,530,693 - BWI76_RS07275 0.25 -2.8 1,530,693 - BWI76_RS07275 0.25 -1.0 1,530,751 - BWI76_RS07275 0.30 +1.4 1,530,770 + BWI76_RS07275 0.32 +0.8 1,530,826 - BWI76_RS07275 0.37 -0.7 1,531,116 - BWI76_RS07275 0.63 +1.3 1,531,150 - +0.6 1,531,195 + BWI76_RS07275 0.70 -2.3 1,531,342 + BWI76_RS07275 0.83 +0.3 1,531,371 + BWI76_RS07275 0.86 -2.9 1,531,371 + BWI76_RS07275 0.86 -0.7 1,531,371 + BWI76_RS07275 0.86 +0.4 1,531,379 - BWI76_RS07275 0.87 -1.6 1,531,620 + +0.4 1,531,658 - BWI76_RS07280 0.13 -0.1 1,531,974 - BWI76_RS07280 0.46 -0.1 1,532,024 + BWI76_RS07280 0.51 +0.4 1,532,068 - BWI76_RS07280 0.56 -0.0 1,532,272 + BWI76_RS07280 0.77 +0.6 1,532,340 + BWI76_RS07280 0.85 +0.5 1,532,395 + -0.5 1,532,405 - -0.1 1,532,405 - -2.6 1,532,410 + +0.0 1,532,416 - -0.3 1,532,418 - -0.2 1,532,446 + -0.1 1,532,477 - +0.4 1,532,501 - +0.7
Or see this region's nucleotide sequence