Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06935 and BWI76_RS06940 are separated by 61 nucleotides BWI76_RS06940 and BWI76_RS06945 are separated by 28 nucleotides BWI76_RS06945 and BWI76_RS06950 overlap by 63 nucleotides BWI76_RS06950 and BWI76_RS06955 overlap by 4 nucleotides
BWI76_RS06935: BWI76_RS06935 - co-chaperone YbbN, at 1,456,641 to 1,457,495
_RS06935
BWI76_RS06940: BWI76_RS06940 - short-chain dehydrogenase/reductase, at 1,457,557 to 1,458,327
_RS06940
BWI76_RS06945: BWI76_RS06945 - multifunctional acyl-CoA thioesterase I/protease I/lysophospholipase L1, at 1,458,356 to 1,459,012
_RS06945
BWI76_RS06950: BWI76_RS06950 - ABC transporter ATP-binding protein, at 1,458,950 to 1,459,636
_RS06950
BWI76_RS06955: BWI76_RS06955 - sugar ABC transporter permease, at 1,459,633 to 1,462,050
_RS06955
Position (kb) 1458
1459
1460 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1457.358 kb on + strand, within BWI76_RS06935 at 1457.451 kb on + strand at 1458.251 kb on - strand at 1458.371 kb on + strand at 1458.371 kb on + strand at 1458.523 kb on + strand, within BWI76_RS06945 at 1458.523 kb on + strand, within BWI76_RS06945 at 1458.550 kb on + strand, within BWI76_RS06945 at 1458.679 kb on + strand, within BWI76_RS06945 at 1458.819 kb on + strand, within BWI76_RS06945 at 1458.827 kb on - strand, within BWI76_RS06945 at 1459.254 kb on + strand, within BWI76_RS06950 at 1459.345 kb on - strand, within BWI76_RS06950 at 1459.445 kb on + strand, within BWI76_RS06950 at 1459.816 kb on - strand at 1459.821 kb on - strand at 1459.849 kb on + strand at 1459.993 kb on - strand, within BWI76_RS06955
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 1,457,358 + BWI76_RS06935 0.84 -2.1 1,457,451 + -0.2 1,458,251 - +0.5 1,458,371 + -0.6 1,458,371 + +0.7 1,458,523 + BWI76_RS06945 0.25 -0.6 1,458,523 + BWI76_RS06945 0.25 -1.6 1,458,550 + BWI76_RS06945 0.30 -0.2 1,458,679 + BWI76_RS06945 0.49 -1.4 1,458,819 + BWI76_RS06945 0.70 -0.6 1,458,827 - BWI76_RS06945 0.72 -2.0 1,459,254 + BWI76_RS06950 0.44 +0.1 1,459,345 - BWI76_RS06950 0.57 -0.2 1,459,445 + BWI76_RS06950 0.72 -3.0 1,459,816 - +0.8 1,459,821 - -0.0 1,459,849 + -0.0 1,459,993 - BWI76_RS06955 0.15 -2.0
Or see this region's nucleotide sequence