Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06325 and BWI76_RS06330 are separated by 148 nucleotides BWI76_RS06330 and BWI76_RS06335 are separated by 45 nucleotides BWI76_RS06335 and BWI76_RS06340 are separated by 295 nucleotides BWI76_RS06340 and BWI76_RS06345 are separated by 147 nucleotides
BWI76_RS06325: BWI76_RS06325 - hypothetical protein, at 1,332,047 to 1,332,424
_RS06325
BWI76_RS06330: BWI76_RS06330 - DNA-binding transcriptional regulator, at 1,332,573 to 1,333,262
_RS06330
BWI76_RS06335: BWI76_RS06335 - nucleoside-specific channel-forming protein Tsx, at 1,333,308 to 1,334,192
_RS06335
BWI76_RS06340: BWI76_RS06340 - hypothetical protein, at 1,334,488 to 1,335,030
_RS06340
BWI76_RS06345: BWI76_RS06345 - transcriptional regulator NrdR, at 1,335,178 to 1,335,627
_RS06345
Position (kb) 1333
1334
1335 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1332.762 kb on + strand, within BWI76_RS06330 at 1332.953 kb on + strand, within BWI76_RS06330 at 1333.131 kb on - strand, within BWI76_RS06330 at 1333.395 kb on + strand at 1333.451 kb on - strand, within BWI76_RS06335 at 1333.461 kb on - strand, within BWI76_RS06335 at 1333.544 kb on + strand, within BWI76_RS06335 at 1333.544 kb on + strand, within BWI76_RS06335 at 1333.751 kb on - strand, within BWI76_RS06335 at 1334.154 kb on + strand at 1334.154 kb on + strand at 1334.154 kb on + strand at 1334.154 kb on + strand at 1334.154 kb on + strand at 1334.205 kb on + strand at 1334.213 kb on - strand at 1334.217 kb on + strand at 1334.267 kb on - strand at 1334.457 kb on - strand at 1334.573 kb on - strand, within BWI76_RS06340
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 1,332,762 + BWI76_RS06330 0.27 +0.0 1,332,953 + BWI76_RS06330 0.55 +0.6 1,333,131 - BWI76_RS06330 0.81 +0.0 1,333,395 + -3.7 1,333,451 - BWI76_RS06335 0.16 -1.2 1,333,461 - BWI76_RS06335 0.17 -2.1 1,333,544 + BWI76_RS06335 0.27 -2.5 1,333,544 + BWI76_RS06335 0.27 +0.1 1,333,751 - BWI76_RS06335 0.50 -0.6 1,334,154 + +0.0 1,334,154 + -1.3 1,334,154 + +0.4 1,334,154 + -0.1 1,334,154 + -0.6 1,334,205 + +1.4 1,334,213 - +0.3 1,334,217 + -0.7 1,334,267 - -0.0 1,334,457 - +0.9 1,334,573 - BWI76_RS06340 0.16 +0.4
Or see this region's nucleotide sequence