Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06210 and BWI76_RS06215 are separated by 37 nucleotides BWI76_RS06215 and BWI76_RS06220 are separated by 288 nucleotides BWI76_RS06220 and BWI76_RS06225 overlap by 4 nucleotides
BWI76_RS06210: BWI76_RS06210 - fructokinase, at 1,301,468 to 1,302,426
_RS06210
BWI76_RS06215: BWI76_RS06215 - NUDIX hydrolase, at 1,302,464 to 1,302,826
_RS06215
BWI76_RS06220: BWI76_RS06220 - exonuclease subunit SbcC, at 1,303,115 to 1,306,249
_RS06220
BWI76_RS06225: BWI76_RS06225 - exonuclease sbcCD subunit D, at 1,306,246 to 1,307,448
_RS06225
Position (kb)
1303
1304
1305
1306
1307 Strain fitness (log2 ratio)
-1
0
1
2 at 1302.125 kb on + strand, within BWI76_RS06210 at 1302.146 kb on + strand, within BWI76_RS06210 at 1302.154 kb on - strand, within BWI76_RS06210 at 1302.308 kb on - strand, within BWI76_RS06210 at 1302.308 kb on - strand, within BWI76_RS06210 at 1302.454 kb on + strand at 1302.490 kb on - strand at 1302.530 kb on + strand, within BWI76_RS06215 at 1303.027 kb on - strand at 1303.354 kb on + strand at 1303.527 kb on - strand, within BWI76_RS06220 at 1303.976 kb on - strand, within BWI76_RS06220 at 1303.997 kb on + strand, within BWI76_RS06220 at 1304.106 kb on - strand, within BWI76_RS06220 at 1304.814 kb on + strand, within BWI76_RS06220 at 1305.012 kb on + strand, within BWI76_RS06220 at 1305.020 kb on - strand, within BWI76_RS06220 at 1305.229 kb on - strand, within BWI76_RS06220 at 1305.804 kb on - strand, within BWI76_RS06220 at 1306.072 kb on - strand at 1306.072 kb on - strand at 1306.174 kb on + strand at 1306.296 kb on + strand at 1306.427 kb on - strand, within BWI76_RS06225 at 1306.854 kb on - strand, within BWI76_RS06225 at 1306.946 kb on - strand, within BWI76_RS06225 at 1307.040 kb on - strand, within BWI76_RS06225
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 1,302,125 + BWI76_RS06210 0.69 -1.0 1,302,146 + BWI76_RS06210 0.71 -0.4 1,302,154 - BWI76_RS06210 0.72 +0.0 1,302,308 - BWI76_RS06210 0.88 -0.8 1,302,308 - BWI76_RS06210 0.88 +0.2 1,302,454 + -0.2 1,302,490 - -0.2 1,302,530 + BWI76_RS06215 0.18 +0.3 1,303,027 - -0.8 1,303,354 + +1.1 1,303,527 - BWI76_RS06220 0.13 +1.5 1,303,976 - BWI76_RS06220 0.27 +1.0 1,303,997 + BWI76_RS06220 0.28 +0.8 1,304,106 - BWI76_RS06220 0.32 +1.4 1,304,814 + BWI76_RS06220 0.54 +0.3 1,305,012 + BWI76_RS06220 0.61 +0.1 1,305,020 - BWI76_RS06220 0.61 +1.6 1,305,229 - BWI76_RS06220 0.67 +0.8 1,305,804 - BWI76_RS06220 0.86 +1.6 1,306,072 - -0.1 1,306,072 - -1.2 1,306,174 + +2.7 1,306,296 + +1.2 1,306,427 - BWI76_RS06225 0.15 +1.5 1,306,854 - BWI76_RS06225 0.51 +2.0 1,306,946 - BWI76_RS06225 0.58 +1.1 1,307,040 - BWI76_RS06225 0.66 +0.7
Or see this region's nucleotide sequence