Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05730 and BWI76_RS05735 are separated by 99 nucleotides BWI76_RS05735 and BWI76_RS05740 overlap by 14 nucleotides BWI76_RS05740 and BWI76_RS05745 are separated by 143 nucleotides BWI76_RS05745 and BWI76_RS05750 are separated by 72 nucleotides
BWI76_RS05730: BWI76_RS05730 - hypothetical protein, at 1,206,461 to 1,207,207
_RS05730
BWI76_RS05735: BWI76_RS05735 - transcriptional regulator, at 1,207,307 to 1,207,675
_RS05735
BWI76_RS05740: BWI76_RS05740 - hypothetical protein, at 1,207,662 to 1,207,898
_RS05740
BWI76_RS05745: BWI76_RS05745 - hypothetical protein, at 1,208,042 to 1,209,280
_RS05745
BWI76_RS05750: BWI76_RS05750 - thiamine biosynthesis protein ThiJ, at 1,209,353 to 1,210,048
_RS05750
Position (kb)
1208
1209
1210 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1207.050 kb on + strand, within BWI76_RS05730 at 1207.050 kb on + strand, within BWI76_RS05730 at 1207.060 kb on - strand, within BWI76_RS05730 at 1207.101 kb on - strand, within BWI76_RS05730 at 1207.102 kb on + strand, within BWI76_RS05730 at 1207.110 kb on - strand, within BWI76_RS05730 at 1207.235 kb on + strand at 1207.241 kb on + strand at 1207.248 kb on + strand at 1207.256 kb on - strand at 1207.373 kb on + strand, within BWI76_RS05735 at 1207.449 kb on + strand, within BWI76_RS05735 at 1207.503 kb on + strand, within BWI76_RS05735 at 1207.503 kb on + strand, within BWI76_RS05735 at 1207.511 kb on - strand, within BWI76_RS05735 at 1207.551 kb on - strand, within BWI76_RS05735 at 1207.556 kb on + strand, within BWI76_RS05735 at 1207.566 kb on + strand, within BWI76_RS05735 at 1207.586 kb on + strand, within BWI76_RS05735 at 1207.588 kb on + strand, within BWI76_RS05735 at 1207.593 kb on + strand, within BWI76_RS05735 at 1207.598 kb on + strand, within BWI76_RS05735 at 1207.598 kb on + strand, within BWI76_RS05735 at 1207.598 kb on + strand, within BWI76_RS05735 at 1207.601 kb on - strand, within BWI76_RS05735 at 1207.603 kb on + strand, within BWI76_RS05735 at 1207.606 kb on - strand, within BWI76_RS05735 at 1207.618 kb on - strand, within BWI76_RS05735 at 1207.654 kb on + strand at 1207.659 kb on + strand at 1207.659 kb on + strand at 1207.667 kb on - strand at 1207.667 kb on - strand at 1207.667 kb on - strand at 1207.678 kb on - strand at 1207.701 kb on - strand, within BWI76_RS05740 at 1207.744 kb on + strand, within BWI76_RS05740 at 1207.768 kb on + strand, within BWI76_RS05740 at 1207.775 kb on + strand, within BWI76_RS05740 at 1207.775 kb on + strand, within BWI76_RS05740 at 1207.811 kb on - strand, within BWI76_RS05740 at 1207.839 kb on - strand, within BWI76_RS05740 at 1207.839 kb on - strand, within BWI76_RS05740 at 1208.008 kb on + strand at 1209.263 kb on + strand at 1209.461 kb on + strand, within BWI76_RS05750 at 1209.473 kb on + strand, within BWI76_RS05750 at 1209.473 kb on + strand, within BWI76_RS05750 at 1209.481 kb on - strand, within BWI76_RS05750 at 1209.481 kb on - strand, within BWI76_RS05750 at 1209.481 kb on - strand, within BWI76_RS05750 at 1209.610 kb on + strand, within BWI76_RS05750 at 1209.616 kb on - strand, within BWI76_RS05750 at 1209.639 kb on + strand, within BWI76_RS05750 at 1209.703 kb on + strand, within BWI76_RS05750 at 1209.736 kb on + strand, within BWI76_RS05750 at 1209.817 kb on + strand, within BWI76_RS05750 at 1209.820 kb on - strand, within BWI76_RS05750 at 1209.825 kb on - strand, within BWI76_RS05750 at 1209.870 kb on - strand, within BWI76_RS05750 at 1209.921 kb on - strand, within BWI76_RS05750 at 1209.967 kb on - strand, within BWI76_RS05750 at 1209.994 kb on + strand at 1209.994 kb on + strand at 1210.012 kb on + strand at 1210.012 kb on + strand at 1210.012 kb on + strand at 1210.020 kb on - strand at 1210.105 kb on + strand at 1210.107 kb on + strand at 1210.113 kb on - strand at 1210.116 kb on + strand at 1210.131 kb on + strand at 1210.139 kb on - strand at 1210.175 kb on - strand at 1210.280 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 1,207,050 + BWI76_RS05730 0.79 -1.3 1,207,050 + BWI76_RS05730 0.79 -0.2 1,207,060 - BWI76_RS05730 0.80 -1.4 1,207,101 - BWI76_RS05730 0.86 -0.1 1,207,102 + BWI76_RS05730 0.86 -0.8 1,207,110 - BWI76_RS05730 0.87 -1.3 1,207,235 + +0.1 1,207,241 + +2.1 1,207,248 + -3.0 1,207,256 - -0.2 1,207,373 + BWI76_RS05735 0.18 -0.7 1,207,449 + BWI76_RS05735 0.38 +1.0 1,207,503 + BWI76_RS05735 0.53 -2.1 1,207,503 + BWI76_RS05735 0.53 -1.2 1,207,511 - BWI76_RS05735 0.55 -1.9 1,207,551 - BWI76_RS05735 0.66 +1.0 1,207,556 + BWI76_RS05735 0.67 -3.6 1,207,566 + BWI76_RS05735 0.70 -1.0 1,207,586 + BWI76_RS05735 0.76 -0.7 1,207,588 + BWI76_RS05735 0.76 -1.9 1,207,593 + BWI76_RS05735 0.78 +0.7 1,207,598 + BWI76_RS05735 0.79 +0.7 1,207,598 + BWI76_RS05735 0.79 +0.1 1,207,598 + BWI76_RS05735 0.79 +2.3 1,207,601 - BWI76_RS05735 0.80 -0.1 1,207,603 + BWI76_RS05735 0.80 -1.9 1,207,606 - BWI76_RS05735 0.81 -2.1 1,207,618 - BWI76_RS05735 0.84 -1.7 1,207,654 + -1.4 1,207,659 + +0.9 1,207,659 + -0.6 1,207,667 - -2.4 1,207,667 - -0.0 1,207,667 - +0.5 1,207,678 - -3.1 1,207,701 - BWI76_RS05740 0.16 -0.4 1,207,744 + BWI76_RS05740 0.35 -1.1 1,207,768 + BWI76_RS05740 0.45 -0.9 1,207,775 + BWI76_RS05740 0.48 -0.5 1,207,775 + BWI76_RS05740 0.48 -0.2 1,207,811 - BWI76_RS05740 0.63 -0.6 1,207,839 - BWI76_RS05740 0.75 -2.6 1,207,839 - BWI76_RS05740 0.75 -1.7 1,208,008 + -3.2 1,209,263 + -2.2 1,209,461 + BWI76_RS05750 0.16 -2.0 1,209,473 + BWI76_RS05750 0.17 -0.5 1,209,473 + BWI76_RS05750 0.17 -1.3 1,209,481 - BWI76_RS05750 0.18 -2.6 1,209,481 - BWI76_RS05750 0.18 +0.4 1,209,481 - BWI76_RS05750 0.18 -1.3 1,209,610 + BWI76_RS05750 0.37 +1.7 1,209,616 - BWI76_RS05750 0.38 -0.6 1,209,639 + BWI76_RS05750 0.41 -2.0 1,209,703 + BWI76_RS05750 0.50 +0.1 1,209,736 + BWI76_RS05750 0.55 +0.2 1,209,817 + BWI76_RS05750 0.67 -0.1 1,209,820 - BWI76_RS05750 0.67 +0.5 1,209,825 - BWI76_RS05750 0.68 -0.6 1,209,870 - BWI76_RS05750 0.74 +1.6 1,209,921 - BWI76_RS05750 0.82 -0.7 1,209,967 - BWI76_RS05750 0.88 -0.6 1,209,994 + +0.0 1,209,994 + -1.1 1,210,012 + +0.3 1,210,012 + +0.3 1,210,012 + -1.8 1,210,020 - -2.4 1,210,105 + +4.4 1,210,107 + -0.5 1,210,113 - -0.2 1,210,116 + +0.8 1,210,131 + -0.1 1,210,139 - +0.5 1,210,175 - -2.3 1,210,280 - -3.6
Or see this region's nucleotide sequence