Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05480

Experiment: KLEB17 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05470 and BWI76_RS05475 are separated by 238 nucleotidesBWI76_RS05475 and BWI76_RS05480 are separated by 43 nucleotidesBWI76_RS05480 and BWI76_RS05485 overlap by 30 nucleotides BWI76_RS05470: BWI76_RS05470 - acyl-CoA dehydrogenase, at 1,146,634 to 1,149,078 _RS05470 BWI76_RS05475: BWI76_RS05475 - phosphoheptose isomerase, at 1,149,317 to 1,149,898 _RS05475 BWI76_RS05480: BWI76_RS05480 - class II glutamine amidotransferase, at 1,149,942 to 1,150,709 _RS05480 BWI76_RS05485: BWI76_RS05485 - transpeptidase, at 1,150,680 to 1,151,420 _RS05485 Position (kb) 1149 1150 1151Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1149.270 kb on + strandat 1150.067 kb on + strand, within BWI76_RS05480at 1150.068 kb on + strand, within BWI76_RS05480at 1150.076 kb on - strand, within BWI76_RS05480at 1150.252 kb on - strand, within BWI76_RS05480at 1150.474 kb on - strand, within BWI76_RS05480at 1151.107 kb on + strand, within BWI76_RS05485at 1151.179 kb on - strand, within BWI76_RS05485at 1151.179 kb on - strand, within BWI76_RS05485at 1151.186 kb on + strand, within BWI76_RS05485at 1151.214 kb on + strand, within BWI76_RS05485at 1151.284 kb on + strand, within BWI76_RS05485at 1151.299 kb on + strand, within BWI76_RS05485at 1151.341 kb on - strand, within BWI76_RS05485at 1151.518 kb on - strandat 1151.699 kb on + strandat 1151.707 kb on - strandat 1151.707 kb on - strandat 1151.707 kb on - strandat 1151.707 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB17 MOI 1
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1,149,270 + -1.4
1,150,067 + BWI76_RS05480 0.16 -0.5
1,150,068 + BWI76_RS05480 0.16 -1.6
1,150,076 - BWI76_RS05480 0.17 -3.0
1,150,252 - BWI76_RS05480 0.40 -0.7
1,150,474 - BWI76_RS05480 0.69 -2.1
1,151,107 + BWI76_RS05485 0.58 +0.0
1,151,179 - BWI76_RS05485 0.67 -4.1
1,151,179 - BWI76_RS05485 0.67 -1.4
1,151,186 + BWI76_RS05485 0.68 -0.3
1,151,214 + BWI76_RS05485 0.72 -3.3
1,151,284 + BWI76_RS05485 0.82 -1.3
1,151,299 + BWI76_RS05485 0.84 -0.4
1,151,341 - BWI76_RS05485 0.89 -0.4
1,151,518 - -0.6
1,151,699 + -0.2
1,151,707 - -0.4
1,151,707 - +0.9
1,151,707 - -1.8
1,151,707 - +0.5

Or see this region's nucleotide sequence