Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04890 and BWI76_RS04895 are separated by 162 nucleotides BWI76_RS04895 and BWI76_RS04900 are separated by 50 nucleotides BWI76_RS04900 and BWI76_RS04905 are separated by 10 nucleotides
BWI76_RS04890: BWI76_RS04890 - dihydrolipoyl dehydrogenase, at 1,024,781 to 1,026,205
_RS04890
BWI76_RS04895: BWI76_RS04895 - hypothetical protein, at 1,026,368 to 1,026,652
_RS04895
BWI76_RS04900: BWI76_RS04900 - DUF2950 domain-containing protein, at 1,026,703 to 1,027,497
_RS04900
BWI76_RS04905: BWI76_RS04905 - DUF3300 domain-containing protein, at 1,027,508 to 1,029,094
_RS04905
Position (kb) 1026
1027 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1026.265 kb on + strand at 1026.273 kb on - strand at 1026.273 kb on - strand at 1026.384 kb on - strand at 1026.400 kb on - strand, within BWI76_RS04895 at 1026.416 kb on + strand, within BWI76_RS04895 at 1026.424 kb on - strand, within BWI76_RS04895 at 1026.944 kb on - strand, within BWI76_RS04900 at 1027.291 kb on - strand, within BWI76_RS04900 at 1027.562 kb on - strand at 1027.630 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 1,026,265 + -2.6 1,026,273 - +0.4 1,026,273 - +0.5 1,026,384 - +1.3 1,026,400 - BWI76_RS04895 0.11 +0.8 1,026,416 + BWI76_RS04895 0.17 +2.3 1,026,424 - BWI76_RS04895 0.20 +0.5 1,026,944 - BWI76_RS04900 0.30 +0.5 1,027,291 - BWI76_RS04900 0.74 -3.5 1,027,562 - +0.5 1,027,630 - -1.6
Or see this region's nucleotide sequence